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Open Access

Cyanidin-3-glucoside protects the photooxidative damage of retinal pigment epithelium cells by regulating sphingolipid signaling and inhibiting MAPK pathway

Tingting Liua,b, Wentao Qib, Wenting Pengb, Jianan Zhangc, Yong Wangb,( )
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Academy of National Food and Strategic Reserves Administration, Beijing 100037, China
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

Peer review under responsibility of Tsinghua University Press.

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Abstract

Cyanidin-3-glucoside (C3G) is the most common anthocyanin in dark grains and berries and is a food functional factor to improve visual health. However, the mechanisms of C3G on blue light-induced retinal pigment epithelial (RPE) cell photooxidative damage needs further exploration. We investigated the effects of C3G on blue light-irradiated A2E-containing RPE cells and explored whether sphingolipid, mitogen-activated protein kinase (MAPK), and mitochondria-mediated pathways are involved in this mechanism. Blue light irradiation led to mitochondria and lysosome damage in RPE cells, whereas C3G preserved mitochondrial morphology and function and maintained the lysosomal integrity. C3G suppressed the phosphorylation of JNK and p38 MAPK and mitochondria-mediated pathways to inhibit RPE cell apoptosis. Lipidomics data showed that C3G protected RPE cells against blue light-induced lipid peroxidation and apoptosis by maintaining sphingolipids balance. C3G significantly inhibited ceramide (Cer d18:0/15:0, Cer d18:0/16:0 and Cer d18:0/18:0) accumulation and elevated galactosylceramide (GalCer d18:1/15:0 and GalCer d18:1/16:0) levels in the irradiated A2E-containing RPE cells. Furthermore, C3G attenuated cell membrane damage by increasing phosphatidylcholine and phosphatidylserine levels. C3G inhibited apoptosis and preserved the structure of mitochondria and lysosome by regulating sphingolipid signaling and suppression of MAPK activation in RPE cells. Thus, dietary supplementation of C3G prevents retinal photooxidative damage.

Keywords

Cyanidin-3-glucosideCeramideMAPK pathwayMitochondria-dependent apoptosisLipidomics analysis

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Food Science and Human Wellness
Volume 13 Issue 2,
March 2024
Pages 621-632
DOI: 10.26599/FSHW.2022.9250053
Cite this article:
Liu T, Qi W, Peng W, et al. Cyanidin-3-glucoside protects the photooxidative damage of retinal pigment epithelium cells by regulating sphingolipid signaling and inhibiting MAPK pathway. Food Science and Human Wellness, 2024, 13(2): 621-632. https://doi.org/10.26599/FSHW.2022.9250053

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Received: 23 October 2022
Revised: 06 November 2022
Accepted: 23 November 2022
Published: 25 September 2023
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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