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• We compared the effects of TB and TBS on the physiological indices of mice.
• TBS contain more bioactive ingredients, less anti-nutritional factors than TB.
• Long-term consumption of TB tends to induce side effects, related to ANFs.
• TB and TBS have different effects on the distribution of gut microbiota.
• Long-term consumption of TBS is safer and effective than TB.
Tartary buckwheat (Fagopyrum tataricum) is a well-known pseudocereal for its health and economic value.However, abundant antinutritional factors (ANFs) reduces its health benefits.As reported, germination can improve the nutritional prof ile of grains.In this study, we systematically evaluate the safety of Tartary buckwheat seeds (TB) and Tartary buckwheat sprouts (TBS) used as high active ingredients.After evaluating nutrition levels, bioactive compounds and ANFs in TBS during germinating, 5th-day TBS were selected as the raw material.C57BL/6J mice were gavaged daily with distilled water, TB, or TBS for 6 weeks.The physiological indices related to ANFs were determined.Results showed that the TB intake tends to generate negative effects on the gut microbiota, and organs.Additionally, upon TB intake, the Fe3+ content in serum, trypsin activity in pancreas and jejunum decreased, while the cytokine, IgE, and histamine levels in serum, water content in faeces, cytokine levels in liver and jejunum increased.Conversely, TBS did not induce any obvious negative effects on the above relevant indices and showed better lipid-lowering effect.Altogether, TBS are safer and more effective as a raw material to produce the functional food for long-term consumption with the intention of preventing and treating hyperlipidaemia.
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