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Open Access

Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway

Xi Lianga,b, Zhe Zhangb, Xiaoying Tianb, Qingyu Cuib, Haiyan Lub, Maozhen Zhaob, Tongjie Liub, Huaxi Yib, Pimin Gongb( ), Lanwei Zhangb( )
Department of Nutrition and Food Hygiene, School of public health, Qingdao University, Qingdao 266071, China
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

Peer review under responsibility of Tsinghua University Press.

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highlights

• Bif. Animalis F1-7 reduced the plaque areas in aorta.

• Bif. Animalis F1-7 down regulated TLR4/NFκB pathway.

• Metabolite ALC is closely related to atherosclerotic inflammatory response.

• Probiotic intervention increases ALC and reduces atherosclerotic inflammation.

Graphical Abstract

Abstract

This study aimed to explore the effect of Bif idobacterium animalis F1-7 on the improvement of atherosclerotic inflammation. Arteriosclerosis model ApoE-/- mice were orally administered with B. animalis F1-7 for 12 weeks. The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages, and downregulated the expression of toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway to reduce the levels of inf lammatory factors. The widely-targeted metabolomics analysis showed that acetyl-L-carnitine (ALC) in the intestine of atherosclerotic mice was signif icantly increased after B. animalis F1-7 intervention. Correlation analysis proved that ALC was associated with atherosclerotic inf lammatory response. By using oxidized low density lipoprotein induced macrophage foam cells, we further verif ied that ALC could reduce lipid accumulation and inf lammatory response in foam cells by downregulating the TLR4/NF-κB pathway. Finally, our results revealed that B. animalis F1-7 upregulated the metabolite ALC to downregulate the inf lammatory responses, leading to the reduction of plaque accumulation of atherosclerosis.

Keywords

Bifidobacterium animalis F1-7AtherosclerosisInflammationMetabonomicsAcetyl-L-carnitine

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Food Science and Human Wellness
Volume 13 Issue 2,
March 2024
Pages 813-822
DOI: 10.26599/FSHW.2022.9250069
Cite this article:
Liang X, Zhang Z, Tian X, et al. Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway. Food Science and Human Wellness, 2024, 13(2): 813-822. https://doi.org/10.26599/FSHW.2022.9250069

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Received: 21 June 2022
Revised: 25 July 2022
Accepted: 16 August 2022
Published: 25 September 2023
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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