AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Food Science and Human Wellness Article
PDF (2.8 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Open Access

Aptasensing biosynthesized phosphatidylserine with a AuNPs nanozyme-based colorimetric aptasensor

Sai Wanga,b,1, Rui Maa,b,1, Chengqiang Lia,b, Ling Zhanga,b, Haiyang Zhanga,b, Xuehan Lia,b, Xiangzhao Maoa,b,c( )
Qingdao Key Laboratory of Food Biotechnology, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China
Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, China
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

1 These authors contributed equally.

Peer review under responsibility of Tsinghua University Press.

Show Author Information

Abstract

Sensitive monitoring of the target products during the biosynthesis process is crucial, and facile analytical approaches are urgently needed. Herein, phosphatidylserine (PS) was chosen as the model target, a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples. A chimeric aptamer was constructed with two homogeneous original PS aptamers. Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme, and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations. The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples. The aptasensor offers good sensitivity and selectivity, under optimal conditions, achieving monitoring and quantitation of PS in the range of 2.5-80.0 μmol/L, with a limit of detection at 536.2 nmol/L. Moreover, the aptasensor provides good accuracy, with comparison rates of 98.17%-106.40%, when compared with the HPLC-ELSD. This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications.

Keywords

PhosphatidylserineChimeric aptamerAuNPs nanozymeColorimetric aptasensor

References

[1]

X. Li, H. Zhao, X. Chen, Screening of marine bioactive antimicrobial compounds for plant pathogens, Mar. Drugs 19(2) (2021) 69. https://doi.org/10.3390/md19020069.
Crossref Google Scholar
[2]

B.G. Andryukov, N.N. Besednova, T.A. Kuznetsova, et al., Sulfated polysaccharides from marine algae as a basis of modern biotechnologies for creating wound dressings: current achievements and future prospects, Biomedicines 8(9) (2020). https://doi.org/10.3390/biomedicines8090301.
Crossref Google Scholar
[3]

X. Shao, X. Wang, K. Zhu, et al., Synthesis of sea cucumber saponins with antitumor activities, J. Org. Chem. 85(19) (2020) 12080-12096. https://doi.org/10.1021/acs.joc.0c01191.
Crossref Google Scholar
[4]

J.W. Zhou, Y. Zhang, J.J. Wu, et al., Proteomic and lipidomic analyses reveal saturated fatty acids, phosphatidylinositol, phosphatidylserine, and associated proteins contributing to intramuscular fat deposition, J. Proteomics. 241 (2021) 104235. https://doi.org/10.1016/j.jprot.2021.104235.
Crossref Google Scholar
[5]

J.E. Vance, G. Tasseva, Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cells, Biochim. Biophys. Acta 1831(3) (2013) 543-554. https://doi.org/10.1016/j.bbalip.2012.08.016.
Crossref Google Scholar
[6]

A. Bruton, J. Nauman, D. Hanes, et al., Phosphatidylserine for the treatment of pediatric attention-deficit/hyperactivity disorder: a systematic review and meta-analysis, J. Altern. Complement. Med. 27(4) (2021) 312-322. https://doi.org/10.1089/acm.2020.0432.
Crossref Google Scholar
[7]

R. Vijay, J.J. Guthmiller, A.J. Sturtz, et al., Hemolysis-associated phosphatidylserine exposure promotes polyclonal plasmablast differentiation, J. Exp. Med. 218(6) (2021). https://doi.org/10.1084/jem.20202359.
Crossref Google Scholar
[8]

Y. Yang, K. Sun, W. Liu, et al., The phosphatidylserine flippase beta-subunit Tmem30a is essential for normal insulin maturation and secretion, Mol. Ther. 29(9) (2021) 2854-2872. https://doi.org/10.1016/j.ymthe.2021.04.026.
Crossref Google Scholar
[9]

M.P. Platre, V. Bayle, L. Armengot, et al., Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine, Science 364(6435) (2019) 57-62. https://doi.org/10.1126/science.aav9959.
Crossref Google Scholar
[10]

A.H. Ali, X. Zou, S.M. Abed, et al., Natural phospholipids: occurrence, biosynthesis, separation, identification, and beneficial health aspects, Crit. Rev. Food Sci. Nutr. 59(2) (2019) 253-275. https://doi.org/10.1080/10408398.2017.1363714.
Crossref Google Scholar
[11]

X.M. Huang, J.G. Zheng, F. Zhang, et al., Optimization of determination of phosphatidylserine in health foods by high performance liquid chromatography-evaporative light scattering detection, J. Food Saf. Food Qual. 10(12) (2019) 3806-3810.
Google Scholar
[12]

C. Tuerk, L. Gold, Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage-T4 DNA-polymerase, Science 249 (1990) 505-510. https://doi.org/10.1126/science.2200121.
Crossref Google Scholar
[13]

A.D. Ellington, J.W. Szostak, In vitro selection of RNA molecules that bind specific ligands, Nature 346 (1990) 818-822. https://doi.org/10.1016/j.gene.2018.03.094.
Crossref Google Scholar
[14]

R. Liu, F. Zhang, Y. Sang, et al., Screening, identification, and application of nucleic acid aptamers applied in food safety biosensing, Trends Food Sci. Tech. 123 (2022) 355-375. https://doi.org/10.1016/j.tifs.2022.03.025.
Crossref Google Scholar
[15]

L. Reynaud, B. Aurélie, J.M. Janot, et al., Discrimination of α-thrombin and γ-thrombin using aptamer-functionalized nanopore sensing, Anal. Chem. 93(22) (2021) 7889-7897. https://doi.org/10.1021/acs.analchem.1c00461.
Crossref Google Scholar
[16]

X. Qi, X. Yan, Y. Zhao, et al., Highly sensitive and specific detection of small molecules using advanced aptasensors based on split aptamers: a review, Trends Analyt. Chem. 133 (2020). https://doi.org/10.1016/j.trac.2020.116069.
Crossref Google Scholar
[17]

F. Yue, H. Li, Q. Kong, et al., Selection of broad-spectrum aptamer and its application in fabrication of aptasensor for detection of aminoglycoside antibiotics residues in milk, Sens. Actuators. B Chem. 351 (2022) 130959. https://doi.org/10.1016/j.snb.2021.130959.
Crossref Google Scholar
[18]

V.S. Raducanu, F. Rashid, M.S. Zaher, et al., A direct fluorescent signal transducer embedded in a DNA aptamer paves the way for versatile metalion detection, Sens. Actuators. B Chem. 304 (2020) 127376. https://doi.org/10.1016/j.snb.2019.127376.
Crossref Google Scholar
[19]

N. Lin, L. Wu, X. Xu, et al., Aptamer generated by cell-SELEX for specific targeting of human glioma cells, ACS Appl. Mater. Interfaces 13(8) (2021) 9306-9315. https://doi.org/10.1021/acsami.0c11878.
Crossref Google Scholar
[20]

P. Mallikaratchy, R.V. Stahelin, Z. Cao, et al., Selection of DNA ligands for protein kinase C-delta, Chem. Commun. 30 (2006) 3229-3231. https://doi.org/10.1039/b604778e.
Crossref Google Scholar
[21]

D. Shangguan, Li Y., Z. Tang, et al., Aptamers evolved from live cells as effective molecular probes for cancer study, Proc. Natl. Acad. Sci. 103(32) (2006) 11838-11843. https://doi.org/10.1073/pnas.0602615103.
Crossref Google Scholar
[22]

D. Pmab, Y.F. Hua, D. Hgab, et al., Aptamer truncation strategy assisted by molecular docking and sensitive detection of T-2 toxin using SYBR Green I as a signal amplifier, Food Chem. 381 (2022) 132171. https://doi.org/10.1016/j.foodchem.2022.132171.
Crossref Google Scholar
[23]

E.S. Báscones, F. Parra, M.J. Lobo-Castaón. Aptamers against viruses: selection strategies and bioanalytical applications, Trends Analyt. Chem. 143 (2021) 116349. https://doi.org/10.1016/j.trac.2021.116349.
Crossref Google Scholar
[24]

J. Zhou, J. Rossi, Aptamers as targeted therapeutics: current potential and challenges, Nat. Rev. Drug Discov. 16(3) (2017) 181-202. https://doi.org/10.1038/nrd.2016.199.
Crossref Google Scholar
[25]

K. Setlem, B. Mondal, R. Shylaja, et al., Dual aptamer-DNAzyme based colorimetric assay for the detection of AFB1 from food and environmental samples, Anal. Biochem. 608(1) (2020) 113874. https://doi.org/10.1016/j.ab.2020.113874.
Crossref Google Scholar
[26]

J. Gao, Z. Chang, R. Tian, et al., Reversible and site-specific immobilization of β2-adrenergic receptor by aptamer-directed method for receptor-drug interaction analysis, J. Chromatogr. A 1622 (2020) 461091. https://doi.org/10.1016/j.chroma.2020.461091.
Crossref Google Scholar
[27]

K. Yu, X. Hai, S. Yue, et al., Glutathione-activated DNA-Au nanomachine as targeted drug delivery platform for imaging-guided combinational cancer therapy, Chem. Eng. J. 419 (2021) 129535. https://doi.org/10.1016/j.cej.2021.129535.
Crossref Google Scholar
[28]

M. Javidi, M.R. Housaindokht, A. Verdian, et al., Detection of chloramphenicol using a novel apta-sensing platform based on aptamer terminal-lock in milk samples, Anal. Chim. Acta 1039 (2018) 116-123. https://doi.org/10.1016/j.aca.2018.07.041.
Crossref Google Scholar
[29]

G.N. Abdelrasoul, A. Anwar, S. Mackay, et al., DNA aptamer-based nonfaradaic impedance biosensor for detecting E. coli, Anal. Chim. Acta 1107(2020) 135-144. https://doi.org/10.1016/j.aca.2020.02.004.
Crossref Google Scholar
[30]

N. Dong, Y. Hu, K. Yang, et al., Development of aptamer-modified SERS nano sensor and oligonucleotide chip to quantitatively detect melamine in milk with high sensitivity, Sens. Actuators. B Chem. 228 (2016) 85-93. https://doi.org/10.1016/j.snb.2015.12.089.
Crossref Google Scholar
[31]

H. Xiong, J. Yan, S. Cai, et al., Cancer protein biomarker discovery based on nucleic acid aptamers, Int. J. Biol. Macromol. 132 (2019) 190-202. https://doi.org/10.1016/j.ijbiomac.2019.03.165.
Crossref Google Scholar
[32]

S. Wang, X. Yan, Y. Yang, et al., Advances and perspectives of aptasensors for the detection of tetracyclines: a class of model compounds of food analysis, Food Chem. 364 (2021) 130361. https://doi.org/10.1016/j.foodchem.2021.130361.
Crossref Google Scholar
[33]

L. Li, Y. Zhao, X. Yan, et al., Development of a terminal-fixed aptamer and a label-free colorimetric aptasensor for highly sensitive detection of saxitoxin, Sens. Actuators. B Chem. 344 (2021) 130320. https://doi.org/10.1016/j.snb.2021.130320.
Crossref Google Scholar
[34]

J. Xie, M.Q. Tang, J. Chen, et al., A sandwich ELISA like detection of C-reactive protein in blood by citicoline-bovine serum albumin conjugate and aptamer-functionalized gold nanoparticles nanozyme, Talanta 217 (2020) 121070. https://doi.org/10.1016/j.talanta.2020.121070.
Crossref Google Scholar
[35]

M. Ashrafuzzaman, C.Y. Tseng, J. Kapty, et al., A computationally designed dna aptamer template with specific binding to phosphatidylserine, Nucleic Acid Ther. 23(6) (2013) 418-426. https://doi.org/10.1089/nat.2013.0415.
Crossref Google Scholar
[36]

X. Yan, X. Qi, Y. Zhao, et al., Development of a label-free colorimetric aptasensor with rationally utilized aptamer for rapid detection of okadaic acid, J. Ocean Univ. China 21(2) (2022) 400-408. https://doi.org/10.1007/s11802-022-4849-x.
Crossref Google Scholar
[37]

H. Zhang, X. Li, Q. Liu, et al., Construction of a super-folder fluorescent protein-guided secretory expression system for the production of phospholipase D in Bacillus subtilis, J. Agric. Food Chem. 69 (2021) 6842-6849. https://doi.org/10.1021/acs.jafc.1c02089.
Crossref Google Scholar
[38]

S. Wang, R. Ma, H. Zhang, et al., Construction of a high affinity aptamer and an aptasensor with chitosan oligosaccharide-AuNPs@Fe2+ nanozyme for highly sensitive detection of phosphatidylserine, Sens. Actuators. B Chem. 362 (2022) 131800. https://doi.org/10.1016/j.snb.2022.131800.
Crossref Google Scholar
[39]

G.L. Ciesielski, V.P. Hytonen, L.S. Kaguni, Biolayer interferometry: a novel method to elucidate protein-protein and protein-DNA interactions in the mitochondrial DNA replisome, Methods Mol. Biol. 1351 (2016) 223-231. https://doi.org/10.1007/978-1-4939-3040-1_17.
Crossref Google Scholar
[40]

X. Feng, Y.H. Zhu, Q. Chen, et al., Determination of phosphatidylserine in softgel capsules of dietary supplements by HPLC-ELSD, China Food Additives 32 (2021) 149-154.
Google Scholar
[41]

F. Zhang, D.C. Zhang, Q. Fu, et al., HPLC-ELSD determination of phosphatidylserine in glutin gelatinized candy with response surface methodology, Chinese Journal of Analysis Laboratory. 38 (2019) 1309-1314.
Google Scholar
[42]

R. Marta, M. Debora, C. Mary, et al., Development and validation of a new high-performance liquid chromatography method for the simultaneous quantification of coenzyme Q10, phosphatidylserine, and vitamin C from a cutting-edge liposomal vehiculization, ACS Omega 22 (2019) 19710-19715. https://doi.org/10.1021/acsomega.9b02456.
Crossref Google Scholar
Food Science and Human Wellness
Volume 13 Issue 2,
March 2024
Pages 823-829
DOI: 10.26599/FSHW.2022.9250070
Cite this article:
Wang S, Ma R, Li C, et al. Aptasensing biosynthesized phosphatidylserine with a AuNPs nanozyme-based colorimetric aptasensor. Food Science and Human Wellness, 2024, 13(2): 823-829. https://doi.org/10.26599/FSHW.2022.9250070

1334

Views

212

Downloads

1

Crossref

1

Web of Science

1

Scopus

0

CSCD

Altmetrics
Received: 21 June 2022
Revised: 29 August 2022
Accepted: 04 October 2022
Published: 25 September 2023
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Return