AI Chat Paper
Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
• A ratiometric fluorescent probe was constructed for hypoxanthine detection.
• The method was based on the enzyme and fluorescence characteristics of Co-doped-g-C3N4.
• The ratiometric fluorescent probe was sensitive, accurate, and reliable.
A ratiometric fluorescent probe for hypoxanthine (Hx) detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride (Co doped g-C3N4). In addition to emitting strong fluorescence, the peroxidase activity of Co doped g-C3N4 can catalyze the reaction of O-phenylenediamine and H2O2 to produce diallyl phthalate which can emit yellow fluorescence at 570 nm. Through the decomposition of Hx by xanthine oxidase, Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I (F570/F370). The linear range was 1.7–272.2 mg/kg (R2 = 0.997), and the detection limit was 1.52 mg/kg (3σ/K, n = 9). The established method was applied to Hx detection in bass, grass carp, and shrimp, and the data were verifi ed by HPLC. The result shows that the established probe is sensitive, accurate, and reliable, and can be used for Hx detection in aquatic products.
S.P. Sun, S. Gu, J.H. Sun, et al., First principles investigation of the electronic properties of graphitic carbon nitride with different building block and sheet staggered arrangement, J. Alloys Compd. 735 (2018) 131-139. https://doi.org/10.1016/j.jallcom.2017.11.061.
M. Mousavi, A. Habibi-Yangjeh, S.R. Pouran, Review on magnetically separable graphitic carbon nitride-based nanocomposites as promising visible-light-driven photocatalysts, J. Mater. Sci. Mater Electron. 29(3) (2018) 1719-1747. https://doi.org/10.1007/s10854-017-8166-x.
Y.D. Li, Z.H. Ruan, Y.Z. He, et al., In situ fabrication of hierarchically porous g-C3N4 and understanding on its enhanced photocatalytic activity based on energy absorption, Appl. Catal. B 236 (2018) 64-75. https://doi.org/10.1016/j.apcatb.2018.04.082.
T. Jiang, G. Jiang, Q. Huang, et al., High-sensitive detection of dopamine using graphitic carbon nitride by electrochemical method, Mater. Res. Bull 74 (2016) 271-277. https://doi.org/10.1016/j.materresbull.2015.10.049.
S.R. Tang, M.L. Wang, G.W. Li, et al., Ultrasensitive colorimetric determination of silver (I) based on the peroxidase mimicking activity of a hybrid material composed of graphitic carbon nitride and platinum nanoparticles, Microchim. Acta 185(5) (2018) 1-7. https://doi.org/10.1007/s00604-018-2816-4.
H. Liu, H. Wang, L. Zhang, et al., Fe(Ⅲ)-oxidized graphitic carbon nitride nanosheets as a sensitive fluorescent sensor for detection and imaging of fluoride ions, Sens. Actuators B 321 (2020) 28630. https://doi.org/10.1016/j.snb.2020.128630.
S. Yu, C. Chen, H. Zhang, et al., Design of high sensitivity graphite carbon nitride/zinc oxide humidity sensor for breath detection, Sens. Actuators B 332 (2021) 129536. https://doi.org/10.1016/j.snb.2021.129536.
M.C. Rong, Z.X. Cai, L. Xie, et al., Study on the ultrahigh quantum yield of fluorescent P, O-g-C3N4 nanodots and its application in cell imaging, Chem. Eur. J. 22(27) (2016) 9387-9395. https://doi.org/10.1002/chem.201601065.
S.F. Kang, L. Zhang, M.F. He, et al., "Alternated cooling and heating” strategy enables rapid fabrication of highly-crystalline g-C3N4 nanosheets for efficient photocatalytic water purification under visible light irradiation, Carbon 137 (2018) 19-30. https://doi.org/10.1016/j.carbon.2018.05.010.
P. Praus, On electronegativity of graphitic carbon nitride, Carbon 172 (2021) 729-732. https://doi.org/10.1016/j.carbon.2020.10.074.
E.G. Gillan, Synthesis of nitrogen-rich carbon nitride networks from an energetic molecular azide precursor,Chem. Mater. 12(12) (2000) 3906-3912. https://doi.org/10.1021/cm000570y.
Z.X. Yang, D.L. Chu, G.R. Jia, et al., Significantly narrowed bandgap and enhanced charge separation in porous, nitrogen-vacancy red g-C3N4 for visible light photocatalytic H2 production, Appl. Surf. Sci. 504 (2020) 144407. https://doi.org/10.1016/j.apsusc.2019.144407.
L.L. Bi, D.D. Meng, Q.J. Bu, et al., Electron acceptor of Ni decorated porous carbon nitride applied in photocatalytic hydrogen production, Phys. Chem. Chem. Phys. 18(46) (2016) 31534-31541. https://doi.org/10.1039/C6CP05618K.
M. Fronczak, M. Krajewska, K. Dembyandand, et al., Extraordinary adsorption of methyl blue onto sodium-doped graphitic carbon nitride, J. Phys. Chem. C. 121(29) (2017) 15756-15766. https://doi.org/10.1021/acs.jpcc.7b03674.
X.H Dai, Z.W. Han, H. Fan, et al., Sulfur doped carbon nitride quantum dots with efficient fluorescent property and their application for bioimaging, J. Nanopart. Res. 20(12) (2018) 1-8. https://doi.org/10.10007/s11051-0184423-y.
D. Masih, Y. Ma, S. Rohani, Ag decorated G-C3N4/black titanium oxides composite for the destruction of environmental pollutant under solar irradiation, Can. J. Chem. Eng. 97(10) (2019) 2632-2641. https://doi.org/10.1002/cjce.23560.
S. Zhao, J.S. Fang, Y.Y. Wang, et al., Construction of three-dimensiona mesoporous carbon nitride with high surface area for efficient visible-lightdriven hydrogen evolution, J. Colloid. Interface Sci. 561 (2020) 601-608. https://doi.org/10.1016/j.jcis.2019.11.035.
X.D. Kang, Y.Y. Kang, X.X. Hong, et al., Improving the photocatalytic activity of graphitic carbon nitride by thermal treatment in a high-pressure hydrogen atmosphere, Prog. Nat. Sci: Mater Int. 28(2) (2018) 183-188. https://doi.org/10.1016/j.pnsc.2018.02.006.
Z.P. Song, Z.H. Li, L.H. Lin, et al., Phenyl-doped graphitic carbon nitride: photoluminescence mechanism and latent fingerprint imaging, Nanoscale 9(45) (2017) 17737-17742. https://doi.org/10.1039/c7nr04845a.
X. Wang, Z.Z. Lin, C.Y. Hong, et al., Colorimetric detection of hypoxanthine in aquatic products based on the enzyme mimic of cobalt-doped carbon nitride, New J. Chem. 45(39) (2021) 18307-18314. https://doi.org/10.1039/D1NJ03467G.
R.C. Nagarajarao, Recent advances in processing and packaging of fishery products: a review, Aquatic. Procedia. 7 (2016) 201-213. https://doi.org/10.1016/j.aqpro.2016.07.028.
P.K. Prabhakar, S. Vatsa, P.P. Srivastav, et al., A comprehensive review on freshness of fish and assessment: analytical methods and recent innovations, Food Res. Int. 133 (2020) 109157. https://doi.org/10.1016/j.foodres.2020.109157.
L. Zhu, S. He, Y. Lu, et al., Metabolomics mechanism of traditional soy sauce associated with fermentation time, Food Sci. Hum. Wellness 11(2) (2022) 297-304. https://doi.org/10.1016/j.fshw.2021.11.023.
H. Hong, X. Yang, Z. You, et al., Visual quality detection of aquatic products using machine vision, Aquacult. Eng. 63 (2014) 62-71. https://doi.org/10.1016/j.aquaeng.2014.10.003
Z.P. Wang, B.K. Ma, C. Shen, et al., Electrochemical biosensing of chilled seafood freshness by xanthine oxidase immobilized on copper-based metalorganic framework nanofiber film, Food Anal. Method 12(8) (2019) 17151724. https://doi.org/10.1007/s12161-019-01513-8.
Z.T. Chen, Y. Lin, X.M. Ma, et al., Multicolor biosensor for fish freshness assessment with the naked eye, Sens. Actuators B 252 (2017) 201-208. https://doi.org/10.1016/j.snb.2017.06.007.
N. Cooper, R. Khosravan, C. Erdmann, et al., Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies, J. Chromatogr. Biomed. Appl. 837(1/2) (2006) 1-10. https://doi.org/10.1016/j.jchromb.2006.02.060.
J.H.T. Luong, K.B. Male, C. Masson, et al., Hypoxanthine ratio determination in fish extract using capillary electrophoresis and immobilized enzymes, J. Food Sci. 57(1) (2010) 77-81. https://doi.org/10.1111/j.13652621.1992.tb05429.x.
Y.N. Zhang, D.J. Hou, Z.L. Wang, et al., Nanomaterial-based dual-emission ratiometric fluorescent sensors for biosensing and cell imaging, Polymers 13(15) (2021) 2540. https://doi.org/10.3390/polym13152540.
G. Sagratini, M. Fernández-Franzón, F.D. Berardinis, et al., Simultaneous determination of eight underivatised biogenic amines in fish by solid phase extraction and liquid chromatography-tandem mass spectrometry, Food Chem. 132(1) (2012) 537-543. https://doi.org/10.1016/j.foodchem.2011.10.054.
L. Zhou, X.F. Xue, J.H. Zhou, et al., Fast determination of adenosine 5’-triphosphate (ATP) and its catabolites in royal jelly using ultraperformance liquid chromatography, J. Agric. Food Chem. 60(36) (2012) 8994-8999. https://doi.org/10.1021/jf3022805.
A.P. Dementjev, A.D. Graaf, M.C.M. de Sanden, et al., X-Ray photoelectron spectroscopy reference data for identification of the C3N4 phase in carbonnitrogen films, Diamond. Relat. Mater. 9(11) (2000) 1904-1907. https://doi.org/10.1016/S0925-9635(00)00345-9.
C.F. Lu, Y. Liu, Q. Wen, et al., Ratiometric fluorescence assay for L-cysteine based on Fe-doped carbon dot nanozymes, Nanotechnology 31(44) (2020) 445703. https://doi.org/10.1088/1361-6528/aba578.
H. Wang, J. Rui, W. Xiao, et al., Enzyme-free ratiometric fluorescence and colorimetric dual read-out assay for glyphosate with ultrathin g-C3N4 nanosheets, Microchem. J. 180 (2022) 107587. https://doi.org/10.1016/j.microc.2022.107587.
974
Views
156
Downloads
2
Crossref
0
Web of Science
0
Scopus
0
CSCD
Altmetrics
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
