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Research Article | Open Access

Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway

Xiaomei Dua,, Kexin Wanga, Xiaoyan Sanga, Xiangxing Menga, Jiao Xieb, Tianxin Wanga, Xiaozhi Liuc,d( ), Qun Huangb( ), Nan Zhanga( ), Hao Wanga( )
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China
The key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guizhou 550025, China
Department of neurosurgery, the Fifth Central Hospital of Tianjin, Tianjin 300450, China
Tianjin Key Laboratory of Epigenetics for Organ Department in Preterm Infants, the Fifth Central Hospital of Tianjin, Tianjin 300450, China

Peer review under responsibility of Tsinghua University Press.

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Abstract

Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties. In this study, PC12, HepG2 cells, and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms. The results showed that naringin inhibited H2O2-induced decline in cell viability and decreased the content of reactive oxygen species in cells. Meanwhile, naringin prolonged the lifespan of f lies, enhanced the abilities of climbing and the resistance to stress, improved the activities of antioxidant enzymes, and decreased malondialdehyde content. Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells. Moreover, naringin down-regulated the mRNA expressions of inr, chico, pi3k, and akt-1, and up-regulated the mRNA expressions of dilp2, dilp3, dilp5, and foxo, thereby activating autophagy-related genes and increasing the number of lysosomes. Furthermore, the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling (IIS) pathway and activating the autophagy pathway, which was consistent with the result of network pharmacological predictions.

Keywords

Drosophila melanogasterHepG2 cellNaringinPC12 cellInsulin signaling (IIS) pathway

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Food Science and Human Wellness
Volume 13 Issue 3,
May 2024
Pages 1231-1245
DOI: 10.26599/FSHW.2022.9250103
Cite this article:
Du X, Wang K, Sang X, et al. Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway. Food Science and Human Wellness, 2024, 13(3): 1231-1245. https://doi.org/10.26599/FSHW.2022.9250103

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Received: 27 July 2022
Revised: 16 October 2022
Accepted: 11 November 2022
Published: 08 February 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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