Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
Search articles, authors, keywords, DOl and etc.
Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties. In this study, PC12, HepG2 cells, and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms. The results showed that naringin inhibited H2O2-induced decline in cell viability and decreased the content of reactive oxygen species in cells. Meanwhile, naringin prolonged the lifespan of f lies, enhanced the abilities of climbing and the resistance to stress, improved the activities of antioxidant enzymes, and decreased malondialdehyde content. Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells. Moreover, naringin down-regulated the mRNA expressions of inr, chico, pi3k, and akt-1, and up-regulated the mRNA expressions of dilp2, dilp3, dilp5, and foxo, thereby activating autophagy-related genes and increasing the number of lysosomes. Furthermore, the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling (IIS) pathway and activating the autophagy pathway, which was consistent with the result of network pharmacological predictions.
D. Saul, R.L. Kosinsky, Epigenetics of aging and aging-associated diseases, Int. J. Mol. Sci. 22 (2021) 1-25. https://doi.org/10.3390/ijms22010401.
N. Bakunina, C.M. Pariante, P.A. Zunszain, Immune mechanisms linked to depression via oxidative stress and neuroprogression, Immunology 144 (2015) 365-373. https://doi.org/10.1111/imm.12443.
O.M. Ighodaro, O.A. Akinloye, First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid, Alexandria J. Med. 54 (2018) 287-293. https://doi.org/10.1016/j.ajme.2017.09.001.
M.K. Chaudhary, S.I. Rizvi, Invertebrate and vertebrate models in aging research, Biomed. Pap. 163 (2019) 114-121. https://doi.org/10.5507/bp.2019.003.
R. Martins, G.J. Lithgow, W. Link, Long live FOXO: unraveling the role of FOXO proteins in aging and longevity, Aging Cell 15 (2016) 196-207. https://doi.org/10.1111/acel.12427.
S. Ahmed, H. Khan, M. Aschner, et al., Therapeutic potential of naringin in neurological disorders, Food Chem. Toxicol. 132 (2019) 110646. https://doi.org/10.1016/j.fct.2019.110646.
O.A. Adebiyi, O.O. Adebiyi, P.M.O. Owira, Naringin reduces hyperglycemia-induced cardiac fibrosis by relieving oxidative stress, PLoS One 11 (2016) e0149890. https://doi.org/10.1371/journal.pone.0149890.
H. Cheng, X. Jiang, Q. Zhang, et al., Naringin inhibits colorectal cancer cell growth by repressing the PI3K/AKT/mTOR signaling pathway, Exp. Ther. Med. 19 (2020) 3798-3804. https://doi.org/10.3892/etm.2020.8649.
Z. Zhang, C. Wang, J. Lin, et al., Therapeutic potential of naringin for intervertebral disc degeneration: involvement of autophagy against oxidative stress-induced apoptosis in nucleus pulposus cells, Am. J. Chinese Med. 46 (2018) 1561-1580. https://doi.org/10.1142/S0192415X18500805.
H. Mu, Q. Zhou, R. Yang, et al., Naringin attenuates high fat diet induced non-alcoholic fatty liver disease and gut bacterial dysbiosis in mice, Front. Microbiol. 11 (2020) 585066. https://doi.org/10.3389/fmicb.2020.585066.
Y. Liu, Y. Liu, Y. Guo, et al., Phlorizin exerts potent effects against aging induced by D-galactose in mice and PC12 cells, Food Funct. 12 (2021) 2148-2160. https://doi.org/10.1039/d0fo02707c.
H. Wang, J. Cheng, H. Wang, et al., Protective effect of apple phlorizin on hydrogen peroxide-induced cell damage in HepG2 cells, J. Food Biochem. 43 (2019) 13052. https://doi.org/10.1111/jfbc.13052.
X. Du, Y. Wang, J. Wang, et al., D-chiro-inositol extends the lifespan of male Drosophila melanogaster better than D-pinitol through insulin signaling and autophagy pathways, Exp. Gerontol. 165 (2022) 111856. https://doi.org/10.1016/j.exger.2022.111856.
X. Zhang, H. Wang, Y. Han, et al., Purple sweet potato extract maintains intestinal homeostasis and extend lifespan through increasing autophagy in female Drosophila melanogaster, J. Food Biochem. 45 (2021) 13861. https://doi.org/10.1111/jfbc.13861.
Y. Wang, H. Wang, T. Ma, et al., Hawthorn extract inhibited the PI3k/Akt pathway to prolong the lifespan of Drosophila melanogaster, J. Food Biochem. (2022) e14169. https://doi.org/10.1111/jfbc.14169.
F. Yang, M. Xiu, S. Yang, et al., Extension of Drosophila lifespan by astragalus polysaccharide through a mechanism dependent on antioxidant and insulin/IGF-1 signaling, Evid. Based Complementary Altern. Med. 2021 (2021) 1-12. https://doi.org/10.1155/2021/6686748.
D. Chattopadhyay, A. Chitnis, A. Talekar, et al., Hormetic efficacy of rutin to promote longevity in Drosophila melanogaster, Biogerontology 18 (2017) 397-411. https://doi.org/10.1007/s10522-017-9700-1.
Y. Han, Y. Guo, S.W. Cui, et al., Purple sweet potato extract extends lifespan by activating autophagy pathway in male Drosophila melanogaster, Exp. Gerontol. 144 (2021) 111190. https://doi.org/10.1016/j.exger.2020.111190.
X. Fan, Y. Zeng, Z. Fan, et al., Dihydromyricetin promotes longevity and activates the transcription factors FOXO and AOP in Drosophila, Aging 13 (2021) 460-476. https://doi.org/10.18632/aging.202156.
X. Feng, L. Chen, W. Guo, et al., Graphene oxide induces p62/SQSTMdependent apoptosis through the impairment of autophagic flux and lysosomal dysfunction in PC12 cells, Acta Biomater. 81 (2018) 278-292. https://doi.org/10.1016/j.actbio.2018.09.057.
X. Song, M. Ni, Y. Zhang, et al., Comparing the inhibitory abilities of epigallocatechin-3-gallate and gallocatechin gallate against tyrosinase and their combined effects with kojic acid, Food Chem. 349 (2021) 129172. https://doi.org/10.1016/j.foodchem.2021.129172.
Z. Yu, S. Wu, W. Zhao, et al., Biological evaluation and interaction mechanism of beta-site APP cleaving enzyme 1 inhibitory pentapeptide from egg albumin, Food Sci. Human Wellness 9 (2020) 162-167. https://doi.org/10.1016/j.fshw.2020.01.004.
Z.Y. Yu, K. Xu, X. Wang, et al., Punicalagin as a novel tyrosinase and melanin inhibitor: inhibitory activity and mechanism, LWT-Food Sci. Technol. 161 (2022) 113318. https://doi.org/10.1016/j.lwt.2022.113318.
A. Nazir, I. Mukhopadhyay, D.K. Saxena, et al., Evaluation of the no observed adverse effect level of solvent dimethyl sulfoxide in Drosophila melanogaster, Toxicol. Mech. Methods. 13 (2003) 147-152. https://doi.org/10.1080/15376510390196527.
D.D. Zhou, M. Luo, S.Y. Huang, et al., Effects and mechanisms of resveratrol on aging and age-related diseases, Oxid. Med. Cell Longev. 2021 (2021) 1-15. https://doi.org/10.1155/2021/9932218.
S. Ahlawat, Asha, K.K. Sharma, Gut-organ axis: a microbial outreach and networking, Lett. Appl. Microbiol. 72 (2021) 636-668. https://doi.org/10.1111/lam.13333.
X. Fan, Q. Liang, T. Lian, et al., Rapamycin preserves gut homeostasis during Drosophila aging, Oncotarget 6(34) (2015) 35274. https://doi.org/10.18632/oncotarget.5895.
D.C. Rubinsztein, G. Mariño, G. Kroemer, Autophagy and aging, Cell 146 (2011) 682-695. https://doi.org/10.1016/j.cell.2011.07.030.
D. González-Ruiz, H. Gohlke, Targeting protein-protein interactions with small molecules: challenges and perspectives for computational binding epitope detection and ligand finding, Curr. Med. Chem. 13(22) (2006) 2607-2625. https://doi.org/10.2174/092986706778201530.
Z. Wu, A. Wu, J. Dong, et al., Skin extract improves muscle function and extends lifespan of a Drosophila model of Parkinson’s disease through activation of mitophagy, Exp. Gerontol. 113 (2018) 10-17. https://doi.org/10.1016/j.exger.2018.09.014.
T. Jagla, M. Dubińska-Magiera, P. Poovathumkadavil, et al., Developmental expression and functions of the small heat shock proteins in Drosophila, Int. J. Mol. Sci. 19 (2018) 3441. https://doi.org/10.3390/ijms19113441.
E. Lashmanova, N. Zemskaya, E. Proshkina, et al., The evaluation of geroprotective effects of selected flavonoids in Drosophila melanogaster and Caenorhabditis elegans, Front. Pharmacol. 8 (2017) 884. https://doi.org/10.3389/fphar.2017.00884.
R. Campion, L. Bloxam, K. Burrow, et al., Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension, Biochem. J. 478 (2021) 4153-4167. https://doi.org/10.1042/BCJ20210432.
W.K. Kang, Y.H. Kim, H.A. Kang, et al., Sir2 phosphorylation through cAMP-PKA and CK2 signaling inhibits the lifespan extension activity of Sir2 in yeast, Elife 4 (2015) e09709. https://doi.org/10.7554/eLife.09709.001.
Y. Ge, H. Chen, J. Wang, et al., Naringenin prolongs lifespan and delays aging mediated by IIS and MAPK in Caenorhabditis elegans, Food Funct. 12 (2021) 12127-12141. https://doi.org/10.1039/d1fo02472h.
E. Untersmayr, A. Brandt, L. Koidl, et al., The intestinal barrier dysfunction as driving factor of inflammaging, Nutrients 14 (2022) 949. https://doi.org/10.3390/nu14050949.
T.S. Agidigbi, C. Kim, Reactive oxygen species in osteoclast differentiation and possible pharmaceutical targets of ros-mediated osteoclast diseases, Int. J. Mol. Sci. 20 (2019) 3576. https://doi.org/10.3390/ijms20143576.
J.H. Park, H.J. Ku, J.K. Kim, et al., Amelioration of high fructose-induced cardiac hypertrophy by naringin, Sci Rep. 8 (2018) 1-11. https://doi.org/10.1038/s41598-018-27788-1.
L. He, T. He, S. Farrar, et al., Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species, Cell Physiol. Biochem. 44 (2017) 532-553. https://doi.org/10.1159/000485089.
Z. Zhang, S. Han, H. Wang, et al., Lutein extends the lifespan of Drosophila melanogaster, Arch. Gerontol. Geriatr. 58 (2014) 153-159. https://doi.org/10.1016/j.archger.2013.07.007.
C. Peng, H.Y.E. Chan, Y.M. Li, et al., Black tea theaflavins extend the lifespan of fruit flies, Exp. Gerontol. 44 (2009) 773-783. https://doi.org/10.1016/j.exger.2009.09.004.
A. Kumar, A. Prakash, S. Dogra, Naringin alleviates cognitive impairment, mitochondrial dysfunction and oxidative stress induced by D-galactose in mice, Food Chem. Toxicol. 48 (2010) 626-632. https://doi.org/10.1016/j.fct.2009.11.043.
J. Xu, Z. Guo, S. Yuan, et al., BCL2L1 is identified as a target of naringenin in regulating ovarian cancer progression, Mol. Cell Biochem. 477 (2022) 1541-1553. https://doi.org/10.1007/s11010-022-04389-1.
X. Lin, G. Smagghe, Roles of the insulin signaling pathway in insect development and organ growth, Peptides 122 (2019) 169923. https://doi.org/10.1016/j.peptides.2018.02.001.
Q. Zhu, Y. Qu, X.G. Zhou, et al., A dihydroflavonoid naringin extends the lifespan of C. elegans and delays the progression of aging-related diseases in PD/AD models via DAF-16, Oxid. Med. Cell Longev. 2020 (2020) 6069354. https://doi.org/10.1155/2020/6069354.
H. Nakatogawa, S. Ohbayashi, M. Sakoh-Nakatogawa, et al., The autophagyrelated protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation, J. Biol. Chem. 287 (2012) 28503-28507. https://doi.org/10.1074/jbc.C112.387514.
P. Codogno, A.J. Meijer, Atg5: more than an autophagy factor, Nat. Cell Biol. 8 (2006) 1045-1047. https://doi.org/10.1038/ncb1006-1045.
S. Meßling, J. Matthias, Q. Xiong, et al., The two Dictyostelium discoideum autophagy 8 proteins have distinct autophagic functions, Eur. J. Cell Biol. 96 (2017) 312-324. https://doi.org/10.1016/j.ejcb.2017.03.014.
D. Chattopadhyay, K. Thirumurugan, Longevity-promoting efficacies of rutin in high fat diet fed Drosophila melanogaster, Biogerontology 21 (2020) 653-668. https://doi.org/10.1007/s10522-020-09882-y.
P. Kharat, P. Sarkar, S. Mouliganesh, et al., Ellagic acid prolongs the lifespan of Drosophila melanogaster, Geroscience 42 (2020) 271-285. https://doi.org/10.1007/s11357-019-00135-6.
S.A. Hollingsworth, R.O. Dror, Molecular dynamics simulation for all, Neuron. 99 (2018) 1129-1143. https://doi.org/10.1016/j.neuron.2018.08.011.
1207
Views
368
Downloads
1
Crossref
1
Web of Science
1
Scopus
0
CSCD
Altmetrics
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).