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Research Article | Open Access

Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites

Ziyan Wanga,b,c,1, Qiuyue Shia,b,c,1, Ying Fengd, Jiaojiao Hana,b,c( ), Chenyang Lua,b,c, Jun Zhoua,b,c, Zhonghua Wange, Xiurong Sua,b,c( )
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315000, China
School of Marine Science, Ningbo University, Ningbo 315000, China
Key Laboratory of Aquaculture Biotechnology (Ningbo University), Ministry of Education, Ningbo University, Ningbo 315000, China
College of Life Sciences, Tonghua Normal University, Tonghua 134000, China
Shandong Beiyou Biotechnology Co., Ltd., Weifang 261000, China

1 These authors contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

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Abstract

Ulcerative colitis (UC) is a recurrent inflammatory bowel disease that imposes a severe burden on families and society. In recent years, exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research interest. This study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV (Pro-Lys-Lys-Val-Val) of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum metabolome. Specif ically, the polypeptide composition of R. esculentum cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α (TNF-α) than the original ligand. Subsequent animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC; affect serum metabolites through the arachidonic acid, glycerophospholipid and linoleic acid metabolism pathways; and further alleviate UC symptoms. This study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.

Keywords

Ulcerative colitisRhopilema esculentum KishinouyeCnidoblastsMarine bioactive polypeptidesMetagenomeSerum metabolome

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Food Science and Human Wellness
Volume 13 Issue 3,
May 2024
Pages 1336-1347
DOI: 10.26599/FSHW.2022.9250112
Cite this article:
Wang Z, Shi Q, Feng Y, et al. Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites. Food Science and Human Wellness, 2024, 13(3): 1336-1347. https://doi.org/10.26599/FSHW.2022.9250112

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Received: 02 August 2022
Revised: 29 August 2022
Accepted: 26 September 2022
Published: 08 February 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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