Highlights
• KRQKYD can ameliorate alcohol-induced HHL-5 hepatocytes damage.
• KRQKYD has strong stability at temperature (20–80 ℃), pH (2–9) and NaCl concentrations (1%–7%).
• KRQKYD is unstable at high temperature and alkaline conditions because of self-assembly aggregation and diacylation.
• KRQKYD is highly resistant to pepsin but can be degraded by trypsin into smaller peptide fragments.
• The trans-epithelial transport mechanism of KRQKYD is paracellular pathways.