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Research Article | Open Access

Anti-obesity effects of fucoidan from Sargassum thunbergii in adipocytes and high fat diet induced obese mice through inhibiting adipogenic specific transcription factor

Hyo-Geun Leea,1,, H.H.A.C.K. Jayawardhanaa,1, Fengqi Yanga,b, D.P. Nagahawaththaa, N.M. Liyanagea, Kyung-Mo Songc, Yun-Sang Choic, Seung-Hong Leed, You-Jin Jeona( ), Min-Cheol Kangc( )
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea
Marine Science Institute, Jeju National University, Jeju 63333, Republic of Korea
Research Group of Food Processing, Korea Food Research Institute, Wanju 55365, Republic of Korea
Department of Pharmoceutical Engineering, Soonchunhyang University, Asan 31538, Republic of Korea

1 These authors contributed this work equally.

Peer review under responsibility of Tsinghua University Press.

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Abstract

The prevalence of obesity has increased and is a health concern worldwide. Due to the concerns regarding synthetic anti-obesity treatments, nowadays natural products become a trend. Previous studies proved that there is a potential to use marine algae as anti-obesity agents. Therefore, in this study, the lipid inhibitory effect of crude polysaccharide of amyloglucosidase-assisted hydrolysate from Sargassum thunbergii (STAC) and its fucoidan fractions (STAFs) on 3T3-L1 cells and high-fat diet (HFD)-induced obese mice were investigated. According to the results, the STAF3, showed the highest xylose content and exhibited significant inhibitory effects on lipid accumulation by downregulating adipogenic and lipogenic proteins in 3T3-L1 cells. Furthermore, oral supplementation with STAC significantly declined gain in body weight and fat weight, and serum lipid contents in an HFD-induced obesity mouse model. Structural and chemical characterizations demonstrated that purified STAF3 has consistent surface morphology and small particle size, with similar structural characteristics as commercial fucoidan. Together, these results indicate that STAC and purified STAF3 from Sargassum thunbergia is a potent source to develop as ananti-obesity agents or functional food products to counter obesity.

Keywords

Anti-obesityFucoidanAnti-steatosisSargassum thunbergii

References

[1]

H. Jebeile, A.S. Kelly, G.O. Malley, et al., Obesity in children and adolescents: epidemiology, causes, assessment, and management, Lancet Diabetes Endocrinol. 10 (2022) 351-365. https://doi.org/10.1016/S2213-8587(22)00047-X.
Crossref Google Scholar
[2]

E.J. Rhee, The influence of obesity and metabolic health on vascular health, Endocrinol. Metab. 37 (2022) 1-8. https://doi.org/10.3803/EnM.2022.101.
Crossref Google Scholar
[3]

B. Abraham, J.H. Sellin, Drug-induced diarrhea, Curr. Gastroenterol Rep. 9 (2007) 365-372. https://doi.org/10.1007/s11894-007-0044-x.
Crossref Google Scholar
[4]

R.M. Cowherd, R.E. Lyle, R.E. McGehee Jr, Molecular regulation of adipocyte differentiation, Semin. Cell Dev. Biol. 10 (1999) 3-10. https://doi.org/10.1006/scdb.1998.0276.
Crossref Google Scholar
[5]

J. Naowaboot, C.H. Chung, P. Pannangpetch, et al., Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes, Nutr. Res. 32 (2012) 39-44. https://doi.org/10.1016/j.nutres.2011.12.003.
Crossref Google Scholar
[6]

V.A. Payne, W.S. Au, C.E. Lowe, et al., C/EBP transcription factors regulate SREBP1c gene expression during adipogenesis, Biochem. J. 425 (2010) 215-224. https://doi.org/10.1042/BJ20091112.
Crossref Google Scholar
[7]

D. Moseti, A. Regassa, W.K. Kim. Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules, Int. J. Mol. Sci. 17 (2016) 124. https://doi.org/10.3390/ijms17010124.
Crossref Google Scholar
[8]

J.S. Park, J.M. Han, D. Surendhiran, et al., Physicochemical and biofunctional properties of Sargassum thunbergii extracts obtained from subcritical water extraction and conventional solvent extraction, J. Supercrit. Fluids 182 (2022) 105535. https://doi.org/10.1016/j.supflu.2022.105535.
Crossref Google Scholar
[9]

S. Murakami, C. Hirazawa, T. Ohya, et al., The edible brown seaweed Sargassum horneri (Turner) C. Agardh ameliorates high-fat diet-induced obesity, diabetes, and hepatic steatosis in mice, Nutrients 13 (2021) 551. https://doi.org/10.3390/nu13020551.
Crossref Google Scholar
[10]

S. Zhou, Q. Zhang, Y. Gao, et al., Sargassum fusiforme together with turmeric extract and pomegranate peel extract alleviates obesity in high fat-fed C57BL/6J mice, Food Funct. 12 (2021) 4654-4669. https://doi.org/10.1039/D0FO03044A.
Crossref Google Scholar
[11]

W. Wijesinghe., Enzyme-assistant extraction (EAE) of bioactive components: a useful approach for recovery of industrially important metabolites from seaweeds: a review. Fitoterapia 83 (2012) 6-12. https://doi.org/10.1016/j.fitote.2011.10.016.
Crossref Google Scholar
[12]

M.C. Kang, H.G. Lee, H.D. Choi, et al., Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii, Int. J. Biol. Macromol. 132 (2019) 142-149. https://doi.org/10.1016/j.ijbiomac.2019.03.178.
Crossref Google Scholar
[13]

P. Cunniff, Official methods of analysis of the Association of Official Analytical Chemists International. Arlingt. AOAC Int. 1995, 11, 6–7.
Google Scholar
[14]

S.S. Nielsen, Phenol-sulfuric acid method for total carbohydrates, Food Analysis Laboratory Manual (2010) 47-53. https://doi.org/10.1007/978-1-4419-1463-7_6.
Crossref Google Scholar
[15]

R. López-Froilán, B. Hernández-Ledesma, M. Cámara, et al., Evaluation of the antioxidant potential of mixed fruit-based beverages: a new insight on the folin-ciocalteu method, Food Anal. Methods. 11 (2018) 2897-2906. https://doi.org/10.1007/s12161-018-1259-1.
Crossref Google Scholar
[16]

K. Dodgson, R. Price. A note on the determination of the ester sulphate content of sulphated polysaccharides, Biochem. J. 84 (1962) 106-110. https://doi.org/10.1042/bj0840106.
Crossref Google Scholar
[17]

H.G. Lee, Y.A. Lu, X. Li, et al., Anti-obesity effects of Grateloupia elliptica, a red seaweed, in mice with high-fat diet-induced obesity via suppression of adipogenic factors in white adipose tissue and increased thermogenic factors in brown adipose tissue, Nutrients 12 (2020) 308. https://doi.org/10.3390/nu12020308.
Crossref Google Scholar
[18]

I.P.S. Fernando, K.K.A. Sanjeewa, H.G. Lee, et al., Fucoidan purified from Sargassum polycystum induces apoptosis through mitochondria-mediated pathway in HL-60 and MCF-7 cells, Mar. Drugs. 18 (2020) 196. https://doi.org/10.3390/md18040196.
Crossref Google Scholar
[19]

I.P.S. Fernando, K.K.A. Sanjeewa, K.W. Samarakoon, et al., A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPSinduced inflammatory responses, Int. J. Biol. Macromol. 104 (2017) 1185-1193. https://doi.org/10.1016/j.ijbiomac.2017.07.031.
Crossref Google Scholar
[20]

S. Palanisamy, M. Vinosha, T. Marudhupandi, et al., In vitro antioxidant and antibacterial activity of sulfated polysaccharides isolated from Spatoglossum asperum, Carbohydr. Polym. 170 (2017) 296-304. https://doi.org/10.1016/j.carbpol.2017.04.085.
Crossref Google Scholar
[21]

J. Anand, M. Sathuvan, G.V. Babu, et al., Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson, Int. J. Biol. Macromol. 111 (2018) 1238-1244. https://doi.org/10.1016/j.ijbiomac.2018.01.057.
Crossref Google Scholar
[22]

K.J. Kim, O.H. Lee, B.Y. Lee, Fucoidan, a sulfated polysaccharide, inhibits adipogenesis through the mitogen-activated protein kinase pathway in 3T3-L1 preadipocytes, Life Sci. 86 (2010) 791-797. https://doi.org/10.1016/j.lfs.2010.03.010.
Crossref Google Scholar
[23]

B. Klop, J.W.F. Elte, M.C. Cabezas, Dyslipidemia in obesity: mechanisms and potential targets, Nutrients 5 (2013) 1218-1240. https://doi.org/10.3390/nu5041218.
Crossref Google Scholar
[24]

I.P.S. Fernando, K.K.A. Sanjeewa, K.W. Samarakoon, et al., FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae, Algae. 32 (2017) 75-86. https://doi.org/10.4490/algae.2017.32.12.1.
Crossref Google Scholar
[25]

H.G. Lee, Y.A. Lu, J.G. Je, et al., Effects of ethanol extracts from Grateloupia elliptica, a red seaweed, and its chlorophyll derivative on 3T3-L1 adipocytes: suppression of lipid accumulation through downregulation of adipogenic protein expression, Mar. Drugs 19 (2021) 91. https://doi.org/10.3390/md19020091.
Crossref Google Scholar
[26]

E. Lim, J.Y. Lim, J.H. Shin, et al., D-xylose suppresses adipogenesis and regulates lipid metabolism genes in high-fat diet-induced obese mice, Nutr. Res. 35 (2015) 626-636. https://doi.org/10.1016/j.nutres.2015.05.012.
Crossref Google Scholar
[27]

S. Lin, T.C. Thomas, L.H. Storlien, et al., Development of high fat dietinduced obesity and leptin resistance in C57Bl/6J mice, Int. J. Obes. 24 (2000) 639-646. https://doi.org/10.1038/sj.ijo.0801209.
Crossref Google Scholar
Food Science and Human Wellness
Volume 13 Issue 3,
May 2024
Pages 1608-1616
DOI: 10.26599/FSHW.2022.9250136
Cite this article:
Lee H-G, Jayawardhana H, Yang F, et al. Anti-obesity effects of fucoidan from Sargassum thunbergii in adipocytes and high fat diet induced obese mice through inhibiting adipogenic specific transcription factor. Food Science and Human Wellness, 2024, 13(3): 1608-1616. https://doi.org/10.26599/FSHW.2022.9250136

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Received: 07 November 2022
Revised: 21 November 2022
Accepted: 29 November 2022
Published: 08 February 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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