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The prevalence of obesity has increased and is a health concern worldwide. Due to the concerns regarding synthetic anti-obesity treatments, nowadays natural products become a trend. Previous studies proved that there is a potential to use marine algae as anti-obesity agents. Therefore, in this study, the lipid inhibitory effect of crude polysaccharide of amyloglucosidase-assisted hydrolysate from Sargassum thunbergii (STAC) and its fucoidan fractions (STAFs) on 3T3-L1 cells and high-fat diet (HFD)-induced obese mice were investigated. According to the results, the STAF3, showed the highest xylose content and exhibited significant inhibitory effects on lipid accumulation by downregulating adipogenic and lipogenic proteins in 3T3-L1 cells. Furthermore, oral supplementation with STAC significantly declined gain in body weight and fat weight, and serum lipid contents in an HFD-induced obesity mouse model. Structural and chemical characterizations demonstrated that purified STAF3 has consistent surface morphology and small particle size, with similar structural characteristics as commercial fucoidan. Together, these results indicate that STAC and purified STAF3 from Sargassum thunbergia is a potent source to develop as ananti-obesity agents or functional food products to counter obesity.
H. Jebeile, A.S. Kelly, G.O. Malley, et al., Obesity in children and adolescents: epidemiology, causes, assessment, and management, Lancet Diabetes Endocrinol. 10 (2022) 351-365. https://doi.org/10.1016/S2213-8587(22)00047-X.
E.J. Rhee, The influence of obesity and metabolic health on vascular health, Endocrinol. Metab. 37 (2022) 1-8. https://doi.org/10.3803/EnM.2022.101.
B. Abraham, J.H. Sellin, Drug-induced diarrhea, Curr. Gastroenterol Rep. 9 (2007) 365-372. https://doi.org/10.1007/s11894-007-0044-x.
R.M. Cowherd, R.E. Lyle, R.E. McGehee Jr, Molecular regulation of adipocyte differentiation, Semin. Cell Dev. Biol. 10 (1999) 3-10. https://doi.org/10.1006/scdb.1998.0276.
J. Naowaboot, C.H. Chung, P. Pannangpetch, et al., Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes, Nutr. Res. 32 (2012) 39-44. https://doi.org/10.1016/j.nutres.2011.12.003.
V.A. Payne, W.S. Au, C.E. Lowe, et al., C/EBP transcription factors regulate SREBP1c gene expression during adipogenesis, Biochem. J. 425 (2010) 215-224. https://doi.org/10.1042/BJ20091112.
D. Moseti, A. Regassa, W.K. Kim. Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules, Int. J. Mol. Sci. 17 (2016) 124. https://doi.org/10.3390/ijms17010124.
J.S. Park, J.M. Han, D. Surendhiran, et al., Physicochemical and biofunctional properties of Sargassum thunbergii extracts obtained from subcritical water extraction and conventional solvent extraction, J. Supercrit. Fluids 182 (2022) 105535. https://doi.org/10.1016/j.supflu.2022.105535.
S. Murakami, C. Hirazawa, T. Ohya, et al., The edible brown seaweed Sargassum horneri (Turner) C. Agardh ameliorates high-fat diet-induced obesity, diabetes, and hepatic steatosis in mice, Nutrients 13 (2021) 551. https://doi.org/10.3390/nu13020551.
S. Zhou, Q. Zhang, Y. Gao, et al., Sargassum fusiforme together with turmeric extract and pomegranate peel extract alleviates obesity in high fat-fed C57BL/6J mice, Food Funct. 12 (2021) 4654-4669. https://doi.org/10.1039/D0FO03044A.
W. Wijesinghe., Enzyme-assistant extraction (EAE) of bioactive components: a useful approach for recovery of industrially important metabolites from seaweeds: a review. Fitoterapia 83 (2012) 6-12. https://doi.org/10.1016/j.fitote.2011.10.016.
M.C. Kang, H.G. Lee, H.D. Choi, et al., Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii, Int. J. Biol. Macromol. 132 (2019) 142-149. https://doi.org/10.1016/j.ijbiomac.2019.03.178.
P. Cunniff, Official methods of analysis of the Association of Official Analytical Chemists International. Arlingt. AOAC Int. 1995, 11, 6–7.
S.S. Nielsen, Phenol-sulfuric acid method for total carbohydrates, Food Analysis Laboratory Manual (2010) 47-53. https://doi.org/10.1007/978-1-4419-1463-7_6.
R. López-Froilán, B. Hernández-Ledesma, M. Cámara, et al., Evaluation of the antioxidant potential of mixed fruit-based beverages: a new insight on the folin-ciocalteu method, Food Anal. Methods. 11 (2018) 2897-2906. https://doi.org/10.1007/s12161-018-1259-1.
K. Dodgson, R. Price. A note on the determination of the ester sulphate content of sulphated polysaccharides, Biochem. J. 84 (1962) 106-110. https://doi.org/10.1042/bj0840106.
H.G. Lee, Y.A. Lu, X. Li, et al., Anti-obesity effects of Grateloupia elliptica, a red seaweed, in mice with high-fat diet-induced obesity via suppression of adipogenic factors in white adipose tissue and increased thermogenic factors in brown adipose tissue, Nutrients 12 (2020) 308. https://doi.org/10.3390/nu12020308.
I.P.S. Fernando, K.K.A. Sanjeewa, H.G. Lee, et al., Fucoidan purified from Sargassum polycystum induces apoptosis through mitochondria-mediated pathway in HL-60 and MCF-7 cells, Mar. Drugs. 18 (2020) 196. https://doi.org/10.3390/md18040196.
I.P.S. Fernando, K.K.A. Sanjeewa, K.W. Samarakoon, et al., A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPSinduced inflammatory responses, Int. J. Biol. Macromol. 104 (2017) 1185-1193. https://doi.org/10.1016/j.ijbiomac.2017.07.031.
S. Palanisamy, M. Vinosha, T. Marudhupandi, et al., In vitro antioxidant and antibacterial activity of sulfated polysaccharides isolated from Spatoglossum asperum, Carbohydr. Polym. 170 (2017) 296-304. https://doi.org/10.1016/j.carbpol.2017.04.085.
J. Anand, M. Sathuvan, G.V. Babu, et al., Bioactive potential and composition analysis of sulfated polysaccharide from Acanthophora spicifera (Vahl) Borgeson, Int. J. Biol. Macromol. 111 (2018) 1238-1244. https://doi.org/10.1016/j.ijbiomac.2018.01.057.
K.J. Kim, O.H. Lee, B.Y. Lee, Fucoidan, a sulfated polysaccharide, inhibits adipogenesis through the mitogen-activated protein kinase pathway in 3T3-L1 preadipocytes, Life Sci. 86 (2010) 791-797. https://doi.org/10.1016/j.lfs.2010.03.010.
B. Klop, J.W.F. Elte, M.C. Cabezas, Dyslipidemia in obesity: mechanisms and potential targets, Nutrients 5 (2013) 1218-1240. https://doi.org/10.3390/nu5041218.
I.P.S. Fernando, K.K.A. Sanjeewa, K.W. Samarakoon, et al., FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae, Algae. 32 (2017) 75-86. https://doi.org/10.4490/algae.2017.32.12.1.
H.G. Lee, Y.A. Lu, J.G. Je, et al., Effects of ethanol extracts from Grateloupia elliptica, a red seaweed, and its chlorophyll derivative on 3T3-L1 adipocytes: suppression of lipid accumulation through downregulation of adipogenic protein expression, Mar. Drugs 19 (2021) 91. https://doi.org/10.3390/md19020091.
E. Lim, J.Y. Lim, J.H. Shin, et al., D-xylose suppresses adipogenesis and regulates lipid metabolism genes in high-fat diet-induced obese mice, Nutr. Res. 35 (2015) 626-636. https://doi.org/10.1016/j.nutres.2015.05.012.
S. Lin, T.C. Thomas, L.H. Storlien, et al., Development of high fat dietinduced obesity and leptin resistance in C57Bl/6J mice, Int. J. Obes. 24 (2000) 639-646. https://doi.org/10.1038/sj.ijo.0801209.
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