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Research Article | Open Access

Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells

Zhuqing Daia,1,, Meimei Niea,1, Ye Chena, Jiangfeng Songa, Yayuan Xua, Zhongyuan Zhanga,b( ), Guodong Zhangc, Shumo Yand, Xing Zhangc, Dajing Lia( )
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
Jiangsu Aland Nutrition Co., Ltd., Taizhou 214500, China
Aland Nutrition Taizhou Co., Ltd., Taizhou 225300, China

1 These authors contributed equally to this study.

Peer review under responsibility of Tsinghua University Press.

Show Author Information

Abstract

In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT) and (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.

Keywords

MechanismAntioxidantLuteinSteviosideHuman retinal pigment epithelial cell

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Food Science and Human Wellness
Volume 13 Issue 3,
May 2024
Pages 1628-1635
DOI: 10.26599/FSHW.2022.9250138
Cite this article:
Dai Z, Nie M, Chen Y, et al. Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells. Food Science and Human Wellness, 2024, 13(3): 1628-1635. https://doi.org/10.26599/FSHW.2022.9250138

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Received: 16 November 2022
Revised: 07 December 2022
Accepted: 19 December 2022
Published: 08 February 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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