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Open Access

Laccase/caffeic acid-catalyzed crosslinking coupled with galactomannan alters the conformational structure of ovalbumin and alleviates Th2-mediated allergic asthma

Ishfaq Ahmeda,b, Suidong Ouyanga, Shengquan Wua,c, Haochang Songa,c, Miaoyuan Zhanga,c, Renxing Luoa,c, Peishan Lua,c, Jiaqi Denga,c, Tingting Zhenga, Yanyan Wanga, Xinguang Liua, Gonghua Huanga( )
Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, China
Haide College, Ocean University of China, Laoshan Campus, Qingdao 266100, China
School of Medical Technology, Guangdong Medical University, Dongguan 523808, China

Peer review under responsibility of Tsinghua University Press.

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Highlights

• Lac/CA crosslinking plus Man conjugation altered the structure of OVA.

• Lac-Man/OVA revealed enhanced the gastrointestinal digestibility.

• Lac-Man/OVA modulated BMDCs maturation and suppressed proinflammatory cytokines.

• Lac/CA crosslinking plus Man conjugation effectively ameliorated allergic asthma.

Graphical Abstract

Abstract

Ovalbumin (OVA) is the major allergenic protein that can induce T helper 2 (Th2)-allergic reactions, for which current treatment options are inadequate. In this study, we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid (Lac/CA)-catalyzed crosslinking in conjunction with galactomannan (Man). The formation of high molecular weight crosslinked polymers and the IgG-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface hydrophobicity. Gastrointestinal digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fl uid (SGF) and simulated intestinal fluid (SIF). Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines (interleukin (IL)-4, IL-5, and IL-13), and upregulated IFN-γ and IL-10 expression. Stimulation of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers (CD80 and CD86) and MHC class Ⅱ molecules, and suppressed the expression levels of proinfl ammatory cytokines. The knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.

Keywords

OvalbuminLaccaseGalactomannanConformational structureAsthma

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Food Science and Human Wellness
Volume 13 Issue 4,
July 2024
Pages 1962-1973
DOI: 10.26599/FSHW.2022.9250163
Cite this article:
Ahmed I, Ouyang S, Wu S, et al. Laccase/caffeic acid-catalyzed crosslinking coupled with galactomannan alters the conformational structure of ovalbumin and alleviates Th2-mediated allergic asthma. Food Science and Human Wellness, 2024, 13(4): 1962-1973. https://doi.org/10.26599/FSHW.2022.9250163

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Received: 09 September 2022
Revised: 21 November 2022
Accepted: 29 January 2023
Published: 20 May 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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