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Open Access

Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite

Huan Lianga,1, Hongcheng Liub,1, Haojian Linc,1, Guobao Ninga, Xiaokang Lua, Siying Mad, Fei Liuc( ), Hui Zhaod( ), Canpeng Lia( )
School of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunming 650091, China
Institute of Quality Standard and Testing Technology, Yunnan Academy of Agricultural Science, Supervision and Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming 650223, China
State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510275, China
Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, China

1 These authors contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

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Highlights

• An electrochemical and colorimetric dual-signal biosensor for Staphylococcus aureus enterotoxin B (SEB) was designed.

• The decreased electrochemical signal caused by methylene blue degradation was proportionate to increased concentration of SEB.

• The colorimetric signal was based on catalytic activity of AuPt@Fe-N-C by oxidation of 3,3′,5,5′-tetramethyl biphenyl.

• The proposed biosensor showed ultrasensitivity, ideal repeatability, and desirable stability.

Graphical Abstract

Abstract

Sensitive detection of Staphylococcus aureus enterotoxin B (SEB) is of importance for preventing food poisoning from threatening human health. In this work, an electrochemical and colorimetric dual-signal detection assay of SEB was developed. The probe (Ab2/AuPt@Fe-N-C) was bound to SEB captured by Ab1, where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal. Furthermore, the probe catalyzed the oxidation of 3, 3’, 5, 5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm. Once the target was captured and formed a sandwich-like complex, the color changed from colorless to blue. SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002–10.0000 and 0.0005–10.0000 ng/mL, with limits of detection of 0.0667 and 0.1670 pg/mL, respectively. The dual-signal biosensor was successfully used to detect SEB in milk and water samples, which has great potential in toxin detection in food and the environment.

Keywords

enterotoxinElectrochemical immunosensorColorimetric assayMOF@borophene compositeDual-functional Fe-N-C signal atom catalyst

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Food Science and Human Wellness
Volume 13 Issue 4,
July 2024
Pages 2025-2035
DOI: 10.26599/FSHW.2022.9250168
Cite this article:
Liang H, Liu H, Lin H, et al. Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite. Food Science and Human Wellness, 2024, 13(4): 2025-2035. https://doi.org/10.26599/FSHW.2022.9250168

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Received: 21 October 2022
Revised: 13 December 2022
Accepted: 30 January 2023
Published: 20 May 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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