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• A non-digestible oligosaccharide (MLO 2-1) was obtained.
• The proposed sugar chain structure of MLO 2-1 was determined.
• MLO 2-1 could tolerate simulated saliva, gastric and intestinal digestion.
• MLO 2-1 displayed the regulatory activity on the gut microbiota, especially Lactobacillus murinus.
• MLO 2-1 showed hypoglycemic activity by selectively accelerating the proliferation of L. murinus.
Two oligosaccharide fractions (MLO 2-1 and 2-2) were purified from enzymatic hydrolysate of mulberry leaf polysaccharide. The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide, which could be degraded by human digestive juice; while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota. Hence, MLO 2-1 was selected for the further analysis. The structure of MLO 2-1 was elucidated as follow: α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1. The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota. Meanwhile, MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids, especially acetate and butyrate. The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated. Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus, a commensal bacterium presented a reduced level in T2DM mice. Animal experiments indicated that MLO 2-1 and L. murinus exhibited hypoglycemic activities. These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L. murinus.
Ö. Aydin, M. Nieuwdorp, V. Gerdes, The gut microbiome as a target for the treatment of type 2 diabetes, Curr. Diab. Rep. 18 (2018) 55. https://doi.org/10.1007/s11892-018-1020-6.
L.P. Zhao, F. Zhang, X.Y. Ding, et al. Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes, Science 359 (2018) 1151-1156. https://doi.org/10.1126/science.aao5774.
J. Aron-Wisnewsky, C. Vigliotti, J. Witjes, et al., Gut microbiota and human NAFLD: disentangling microbial signatures from metabolic disorders, Nat. Rev. Gastro. Hepat. 17 (2020) 279-297. https://doi.org/10.1038/s41575-020-0269-9.
B.L. Zhu, X. Wang, L.J. Li, Human gut microbiome: the second genome of human body, Protein Cell. 1 (2010) 718-725. https://doi.org/10.1007/s13238-010-0093-z.
P. J. Turnbaugh, M. Hamady, T. Yatsunenko, et al., A core gut microbiome in obese and lean twins, Nature 457 (2009) 480-484. https://doi.org/10.1038/nature07540.
C. Manichanh, L. Rigottier-Gois, E. Bonnaud, et al., Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach, Gut 55 (2006) 205. https://doi.org/10.1136/gut.2005.073817.
E. Le Chatelier, T. Nielsen, J. Qin, et al. Richness of human gut microbiome correlates with metabolic markers, Nature 500 (2013) 541-546. https://doi.org/10.1038/nature12506.
A. Cotillard, S. P. Kennedy, L. C. Kong, et al., Dietary intervention impact on gut microbial gene richness, Nature 500 (2013) 585-588. https://doi.org/10.1038/nature12480.
J. Qin, Y. Li, Z. Cai, et al., A metagenome-wide association study of gut microbiota in type 2 diabetes, Nature 490 (2012) 55-60. https://doi.org/10.1038/nature11450.
X.Y. Zhang, D.Q. Shen, Z.W. Fang, et al., Human gut microbiota changes reveal the progression of glucose intolerance, PLoS ONE 8 (2013) 71108. https://doi.org/10.1371/journal.pone.0071108.
H. Plovier, A. Everard, C. Druart, et al., A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice, Nat. Med. 23 (2017) 107-113. https://doi.org/10.1038/nm.4236.
H.F. Liu, X.Y. Zeng, J.Y. Huang, et al., Dietary fiber extracted from pomelo fruitlets promotes intestinal functions, both in vitro and in vivo, Carbohydr. Polym. 252 (2021) 117186. https://doi.org/10.1016/j.carbpol.2020.117186.
T.M. Yuan, Z.J. Yin, Z.X. Yan, et al., Tetrahydrocurcumin ameliorates diabetes profiles of db/db mice by altering the composition of gut microbiota and up-regulating the expression of GLP-1 in the pancreas, Fitoterapia 146 (2020) 104665. https://doi.org/10.1016/j.fitote.2020.104665.
M. Conlon, A. Bird, The impact of diet and lifestyle on gut microbiota and human health, Nutrients 7 (2014) 17-44. https://doi.org/10.3390/nu7010017.
Q.X. Nie, H.H. Chen, J.L. Hu, et al., Effects of nondigestible oligosaccharides on obesity, Annu. Rev. Food Sci. T. 11 (2020) 205-233. https://doi.org/10.1146/annurev-food-032519-051743.
P. Wen, T.G. Hu, R. J. Linhardt, et al., Mulberry: a review of bioactive compounds and advanced processing technology, Trends Food Sci. Tech. 83 (2019) 138-158. https://doi.org/10.1016/j.tifs.2018.11.017.
M.J. Yebra, V. Monedero, M. Zuniga, et al., Molecular analysis of the glucose-specific phosphoenolpyruvate: sugar phosphotransferase system from Lactobacillus casei and its links with the control of sugar metabolism, Microbiology 152 (2006) 95-104. https://doi.org/10.1099/mic.0.28293-0.
T.G. Hu, H. Wu, Y.S. Yu, et al. Preparation, structural characterization and prebiotic potential of mulberry leaf oligosaccharides, Food Funct. 13 (2022) 5287-5298. https://doi.org/10.1039/d1fo04048k.
Q.X. Yuan, Y.F. Xie, W. Wang, et al., Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L. ) leaves, Carbohydr. Polym. 128 (2015) 52-62. https://doi.org/10.1016/j.carbpol.2015.04.028.
M. Sun, C. Sun, H.G. Xie, et al., A simple method to calculate the degree of polymerization of alginate oligosaccharides and low molecular weight alginates, Carbohydr. Res. 486 (2019) 107856. https://doi.org/10.1016/j.carres.2019.107856.
J.L. Hu, S.P. Nie, F.F. Min, et al., Artificial simulated saliva, gastric and intestinal digestion of polysaccharide from the seeds of Plantago asiatica L, Carbohydr. Polym. 92 (2013) 1143-1450. https://doi.org/10.1016/j.carbpol.2012.10.072.
C. Tedeschi, V. Clement, M. Rouvet, et al., Dissolution tests as a tool for predicting bioaccessibility of nutrients during digestion, Food Hydrocoll. 23 (2009) 1228-1235. https://doi.org/10.1016/j.foodhyd.2008.09.012.
T.G. Hu, P. Wen, W.Z. Shen, et al., Effect of 1-deoxynojirimycin isolated from mulberry leaves on glucose metabolism and gut microbiota in a streptozotocin-induced diabetic mouse model, J. Nat. Prod. 82 (2019) 2189-2200. https://doi.org/10.1021/acs.jnatprod.9b00205.
T.G. Hu, P. Wen, H.Z. Fu, et al., Protective effect of mulberry (Morus atropurpurea) fruit against diphenoxylate-induced constipation in mice through the modulation of gut microbiota, Food Funct. 10 (2019) 1513-1528. https://doi.org/10.1039/c9fo00132h.
Y.J. Bai, F. Huang, R.F. Zhang, et al. Longan pulp polysaccharides relieve intestinal injury in vivo and in vitro by promoting tight junction expression, Carbohydr. Polym. 229 (2020) 115475. https://doi.org/10.1016/j.carbpol.2019.115475.
J.W. Li, L.Z. Ai, Q. Yang, et al., Isolation and structural characterization of a polysaccharide from fruits of Zizyphus jujuba cv. Junzao, Int. J. Biol. Macromol. 55 (2013) 83-87. https://doi.org/10.1016/j.ijbiomac.2012.12.017.
Y.L. Fan, W.H. Wang, W. Song, et al., Partial characterization and anti-tumor activity of an acidic polysaccharide from Gracilaria lemaneiformis, Carbohydr. Polym. 88 (2012) 1313-1318. https://doi.org/10.1016/j.carbpol.2012.02.014.
L.L. Cai, S.S. Zou, D.P. Liang, et al., Structural characterization, antioxidant and hepatoprotective activities of polysaccharides from Sophorae tonkinensis Radix, Carbohydr. Polym. 184 (2018) 354-365. https://doi.org/10.1016/j.carbpol.2017.12.083.
M.K. Patel, B. Tanna, A. Mishra, et al., Physicochemical characterization, antioxidant and anti-proliferative activities of a polysaccharide extracted from psyllium (P. ovata) leaves, Int. J. Biol. Macromol. 118 (2018) 976-987. https://doi.org/10.1016/j.ijbiomac.2018.06.139.
N. Yang, N.N. Zhang, Y.M. Jin, et al., Development of a fluidic system for efficient extraction of mulberry leaves polysaccharide using induced electric fields, Sep. Purif. Technol. 172 (2017) 318-325. https://doi.org/10.1016/j.seppur.2016.08.025.
A. Klaus, M. Kozarski, J. Vunduk, et al., Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa, Food Res. Int. 67 (2015) 272-283. https://doi.org/10.1016/j.foodres.2014.11.035.
B. Gullon, G. Eibes, I. Davila, et al., Hydrothermal treatment of chestnut shells (Castanea sativa) to produce oligosaccharides and antioxidant compounds, Carbohydr. Polym. 192 (2018) 75-83. https://doi.org/10.1016/j.carbpol.2018.03.051.
S.M. Van Ruth, J. P Roozen, Influence of mastication and saliva on aroma release in a model mouth system, Food Chem. 71 (2000) 339-345. https://doi.org/10.1016/S0308-8146(00)00186-2.
L.G. Chen, W. Xu, D. Chen, et al. Digestibility of sulfated polysaccharide from the brown seaweed Ascophyllum nodosum and its effect on the human gut microbiota in vitro, Int. J. Biol. Macromol. 112 (2018) 1055-1061. https://doi.org/10.1016/j.ijbiomac.2018.01.183.
C. Chen, B. Zhang, X. Fu, et al., The digestibility of mulberry fruit polysaccharides and its impact on lipolysis under simulated saliva, gastric and intestinal conditions, Food Hydrocoll. 58 (2016) 171-178. https://doi.org/10.1016/j.foodhyd.2016.02.033.
I.F. Olawuyi, W.Y. Lee, Structural characterization, functional properties and antioxidant activities of polysaccharide extract obtained from okra leaves (Abelmoschus esculentus), Food Chem. 354 (2021) 129437. https://doi.org/10.1016/j.foodchem.2021.129437.
P.E. Jansson, L. Kenne, G. Widmalm, Computer-assisted structural analysis of polysaccharides with an extended version of CASPER using 1H- and 13C-NMR data, Carbohydr. Res. 188 (1989) 169-191. https://doi.org/10.1016/0008-6215(89)84069-8.
H. Kono, S. Kawano, T. Erata, et al., Regioselective syntheses of new tri-and tetrasaccharides by transglycosylation of trichoderma viride β-glucosidase, J. Carbohydr. Chem. 19 (2000) 127-140. https://doi.org/10.1080/07328300008544070.
X.J. Li, W.R. Bao, C.H. Leung, et al., Chemical structure and immunomodulating activities of an α-glucan purified from Lobelia chinensis Lour, Molecules 21 (2016) 779. https://doi.org/10.3390/molecules21060779.
A. Percy, H. Ono, D. Watt, et al., Synthesis of β-D-glucopyranosyl-(1→4)-D-arabinose, β-D-glucopyranosyl-(1→4)-l-fucose and β-D-glucopyranosyl-(1→4)-D-altrose catalysed by cellobiose phosphorylase from Cellvibrio gilvus, Carbohydr. Res. 305 (1997) 543-548. https://doi.org/10.1016/S0008-6215(97)00247-4.
C. Chen, L.L. Wang, Y.Q. Lu, et al., Comparative transcriptional analysis of Lactobacillus plantarum and its ccpA-knockout mutant under galactooligosaccharides and glucose conditions, Front. Microbiol. 10 (2019) 1584. https://doi.org/10.3389/fmicb.2019.01584.
K. Okano, S. Yoshida, T. Tanaka, et al., Homo-D-lactic acid fermentation from arabinose by redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-lactate dehydrogenase gene-deficient, Appl. Environ. Microbiol. 75 (2009) 5175-5178. https://doi.org/10.1128/AEM.00573-09.
A. Koh, F. De Vadder, P. Kovatcheva-Datchary, et al., From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites, Cell 165 (2016) 1332-1345. https://doi.org/10.1016/j.cell.2016.05.041.
H.S. Sung, Y.L. Jo, Purification and characterization of an antibacterial substance from Aerococcus urinaeequi strain HS36, J. Microbiol. Biotechnol. 30 (2020) 93-100. https://doi.org/10.4014/jmb.1910.10015.
S.J. Yue, D. Zhao, C.X. Peng, et al., Effects of theabrownin on serum metabolites and gut microbiome in rats with a high-sugar diet, Food Funct. 10 (2019) 7063-7080. https://doi.org/10.1039/c9fo01334b.
F.W. Pan, L.Y. Zhang, M. Li, et al., Predominant gut Lactobacillus murinus strain mediates anti-inflammaging effects in calorie-restricted mice, Microbiome 6 (2018). https://doi.org/10.1186/s40168-018-0440-5.
L. Delucchi, M. Fraga, K. Perelmuter, et al., Effect of native Lactobacillus murinus LbP2 administration on total fecal IgA in healthy dogs, Can. J. Vet. Res. 78 (2014) 153-155.
H.F. Ge, Z.Z. Cai, J.L. Chai, et al., Egg white peptides ameliorate dextran sulfate sodium-induced acute colitis symptoms by inhibiting the production of pro-inflammatory cytokines and modulation of gut microbiota composition, Food Chem. 360 (2021) 129981. https://doi.org/10.1016/j.foodchem.2021.129981.
C. De Luca, J.M. Olefsky, Inflammation and insulin resistance, FEBS Lett. 582 (2008) 97-105. https://doi.org/10.1016/j.febslet.2007.11.057.
N. Rodríguez-Medina, H. Barrios-Camacho, J. Duran-Bedolla, et al., Klebsiella variicola: an emerging pathogen in humans, Emerg. Microbe. Infec. 8 (2019) 973-988. https://doi.org/10.1080/22221751.2019.1634981.
A. Ponte, C. Costa. Abcessos hepáticos múltiplos devido a Morganella morganii, Acta Med. Port. 28 (2015) 539. https://doi.org/10.20344/amp.5584.
A. Gomez-Nguyen, A.R. Basson, L. Dark-Fleury, et al., Parabacteroides distasonis induces depressive-like behavior in a mouse model of Crohn's disease, Brain Behav. Immun. 98 (2021) 245-250. https://doi.org/10.1016/j.bbi.2021.08.218.
M.E. Perez-Muñoz, K. Bergstrom, V. Peng, et al., Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis, Gut Microbes. 5 (2014) 286-485. https://doi.org/10.4161/gmic.28622.
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