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• The three purified honeysuckle polysaccharides differed in structural features.
• They showed excellent anti-inflammatory activities via NF-κB/MAPK signaling pathways.
• The molecular characteristics and chemical compositions greatly affected bioactivity.
• LJP-2 mainly consisted of →5)-α-L-Araf (1→, →4)-α-L-GalpA (1→ and →2)-α-L-Rhap (1→.
This study demonstrates the feasibility of producing three polysaccharides (neutral LJP -1, acidic LJP-2 and acidic LJP-3) with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera japonica. The three polysaccharides differed in chemical composition, molecular weight (Mw) distribution, glycosidic linkage pattern, functional groups and morphology. They exhibited excellent protective effects (in a dose-dependent manner) in lipopolysaccharide-injured RAW264.7 macrophages and CuSO4-damaged zebrafish via reducing NO production and inhibiting the overexpressions of inflammation-related transcription factors, inflammatory proteins and cytokines in the NF-κB/MAPK signaling pathways. Their anti-inflammatory effects varied owing to their different molecular characteristics and chemical compositions. Overall, LJP-2 at 400 μg/mL was the most effective. LJP-2 consisted mainly of →5)-α-L-Araf (1→, →4)-α-L-GalpA (1→ and →2)-α-L-Rhap (1→ residues with terminal T-β-D-Glcp. Thus, honeysuckle flowers are good sources of anti-inflammatory polysaccharides, and precise fractionation enables the production of potent anti-inflammatory agents for the development of functional foods and healthcare products.
X.F. Shang, H. Pan, M.X. Li, et al., Lonicera japonica Thunb.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine, J. Ethnopharmacol. 138(1) (2011) 1-21. https://doi.org/10.1016/j.jep.2011.08.016.
D.Y. Su, S. Li, W. Zhang, et al., Structural elucidation of a polysaccharide from Lonicera japonica flowers, and its neuroprotective effect on cerebral ischemia-reperfusion injury in rat, Int. J. Biol. Macromol. 99 (2017) 350-357. https://doi.org/10.1016/j.ijbiomac.2017.02.096.
J. Lee, Y. Li, H. Chen, et al., Protective effects of luteolin against lipopolysaccharide-induced acute lung injury involves inhibition of MEK/ ERK and PI3K/Akt pathways in neutrophils, Acta Pharmacol. Sin. 31(7) (2010) 831-838. https://doi.org/10.1038/aps.2010.62.
L.Y. Lin, P.P. Wang, Z.Y. Du, et al., Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity, Int. J. Biol. Macromol. 88 (2016) 130-137. https://doi.org/10.1016/j.ijbiomac.2016.03.025.
X.Y. Bai, Y. Chai, W.L Shi, et al., Lonicera japonica polysaccharides attenuate ovalbumin-induced allergic rhinitis by regulation of Th17 cells in BALB/c mice, J. Funct. Foods 65 (2020) 103758. https://doi.org/10.1016/j.jff.2019.103758.
P.P. Wang, W.F. Liao, J.P Fang, et al., A glucan isolated from flowers of Lonicera japonica Thunb. inhibits aggregation and neurotoxicity of Aβ42, Carbohyd. Polym. 110 (2014) 142-147. https://doi.org/10.1016/j.carbpol.2014.03.060.
Q. Liu, J.P Fang, P.P Wang, et al., Characterization of a pectin from Lonicera japonica Thunb. and its inhibition effect on Aβ42 aggregation and promotion of neuritogenesis, Int. J. Biol. Macromol. 107 (2018) 112-120. https://doi.org/10.1016/j.ijbiomac.2017.08.154.
T. Zhang, H.P. Liu, X.Y. Bai, et al., Fractionation and antioxidant activities of the water-soluble polysaccharides from Lonicera japonica Thunb, Int. J. Biol. Macromol. 151 (2020) 1058-1066. https://doi.org/10.1016/j.ijbiomac.2019.10.147.
J. Lee, W. Ko, Y. Kim, et al., Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-κB activation through reducing I-κBα degradation in rat liver, Int. J. Mol. Med. 7(1) (2001) 79-162. https://doi.org/10.3892/ijmm.7.1.79.
J. Tae, S. Han, J. Yoo, et al., Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema, Clin. Chim. Acta 330(1/2) (2003) 165-171. https://doi.org/10.1016/S0009-8981(03)00017-2.
L.Y. Li, Z.C. Qiu, H.J. Dong, et al., Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: a comparison, Int. J. Biol. Macromol. 182 (2021) 187-196. https://doi.org/10.1016/j.ijbiomac.2021.03.177.
C. Liu, Z.C. Qiu, D.Y. Gu, et al., A novel anti-inflammatory polysaccharide from blackened jujube: structural features and protective effect on dextran sulfate sodium-induced colitic mice, Food Chem. 405 (2022) 134869. https://doi.org/10.1016/j.foodchem.2022.134869.
S.S. Zhu, Z.C. Qiu, X.G. Qiao, et al., Creating burdock polysaccharide-oleanolic acid-ursolic acid nanoparticles to deliver enhanced anti-inflammatory effects: fabrication, structural characterization and property evaluation, Food Sci. Hum. wellness. 12(2) (2022) 454-466. https://doi.org/10.1016/j.fshw.2022.07.047.
L.L. Yuan, Z.C. Qiu, Y.M. Yang, et al., Preparation, structural characterization and antioxidant activity of water-soluble polysaccharides and purified fractions from blackened jujube by an activity-oriented approach, Food Chem. 385 (2022) 132637. https://doi.org/10.1016/j.foodchem.2022.132637.
Y.M Zhang, X.L. Pan, S.Q. Ran, et al., Purification, structural elucidation and anti-inflammatory activity in vitro of polysaccharides from Smilax china L., Int. J. Biol. Macromol. 139 (2019) 233-243. https://doi.org/10.1016/j.ijbiomac.2019.07.209.
G. Waterhouse, W. Wang, D. Sun-Waterhouse, Stability of canola oil encapsulated by co-extrusion technology: effect of quercetin addition to alginate shell or oil core, Food Chem. 142 (2014) 27-38. https://doi.org/10.1016/j.foodchem.2013.07.035.
Y.M. Yang, Z.C. Qiu, L.Y. Li, et al., Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from Ziziphus jujuba cv. Hamidazao: a comparison, Carbohyd. Polym. 261 (2021) 117879. https://doi.org/10.1016/j.carbpol.2021.117879.
Y.Q. Sun, J.X. Huo, S. Zhong, et al., Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii, Carbohyd. Polym. 268 (2021) 118214. https://doi.org/10.1016/j.carbpol.2021.118214.
X.X. Liu, H.M. Liu, Y.Y Yan, et al., Structural characterization and antioxidant activity of polysaccharides extracted from jujube using subcritical water, LWT 117 (2020) 108645. https://doi.org/10.1016/j.lwt.2019.108645.
Z.C. Qiu, Y.T. Qiao, B. Zhang, et al., Bioactive polysaccharides and oligosaccharides from garlic (Allium sativum L.): production, physicochemical and biological properties, and structure-function relationships, Compr. Rev. Food Sci. F. 21(4) (2022) 3033-3095. https://doi.org/10.1111/1541-4337.12972.
X.Q. Xu, Y.G. Chang, C.H. Xue, et al., Gastric protective activities of sea cucumber fucoidans with different molecular weight and chain conformations: a structure-activity relationship investigation, J. Agr. Food Chem. 66(32) (2018) 8615-8622. https://doi.org/10.1021/acs.jafc.8b01497.
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