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Open Access

Structural characterization and prebiotic potential of polysaccharides from Polygonatum sibiricum

Zihan Qia,b,#, Tiexiang Gaob,#, Jingjing Lia, Shuhan Zhoua, Zhigang Zhanga, Mingzhu Yina, Haiming Hua( ), Hongtao Liua( )
College of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China

# These authors contributed equally to this work and should be considered co-fi rst authors.

Peer review under responsibility of Tsinghua University Press.

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Highlights

• A low-molecular-weight polysaccharide was isolated from Polygonatum sibiricum.

• Polygonatum sibiricum polysaccharide (PSP) was a glucofructan-type polysaccharide.

• PSP was indigestible in saliva-gastrointestinal digestion but could be degraded and utilized by intestinal bacteria.

• The diversity and abundance of gut microbiota were significantly affected by PSP.

• The PSP fermentation broth displayed an excellent scavenging effect on free radicals, including DPPH, superoxide, and hydroxyl radicals

Graphical Abstract

Abstract

Polygonatum sibiricum has been widely used due to its excellent biological activities. We prepared a novel polysaccharide from P. sibiricum (PSP) in this study. According a monosaccharide composition analysis, PSP was mainly composed of fructose and glucose with a molar percentage of 93.81:5.12. The main linkage types were identified as α-D-Glcp-1→ and →2-β-D-Fruf-1→. The molecular weight of PSP showed no significant change after simulated salivary and gastrointestinal digestion. However, PSP could be broken down by intestinal bacteria. Our findings revealed that PSP administration increased the abundance of probiotics such as Bifi dobacterium. Furthermore, the results showed that gut microbes could utilize PSP to produce short-chain fatty acids including acetic acid, propionic acid, and butyric acid. Also, the PSP fermentation broth displayed an excellent scavenging effect on free radicals, including 2, 2-diphenyl-1-picrylhydrazyl radical, superoxide radical, and hydroxyl radical. In summary, this study will help to promote the application of PSP as prebiotics in functional food and the medical industry.

Keywords

Dietary polysaccharidesPrebioticGut microbiotaShort-chain fatty acids

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Food Science and Human Wellness
Volume 13 Issue 4,
July 2024
Pages 2208-2220
DOI: 10.26599/FSHW.2022.9250184
Cite this article:
Qi Z, Gao T, Li J, et al. Structural characterization and prebiotic potential of polysaccharides from Polygonatum sibiricum. Food Science and Human Wellness, 2024, 13(4): 2208-2220. https://doi.org/10.26599/FSHW.2022.9250184

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Received: 08 December 2022
Revised: 02 January 2023
Accepted: 26 January 2023
Published: 20 May 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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