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Open Access

Effects of low molecular weight polysaccharide from Sargassum thunbergii against palmitic acid-induced intracellular lipid accumulation in 3T3-L1 adipocyte and HepG2 cells

Hyo-Geun Leea,, D.P. Nagahawattaa, M.J.M.S. Kureraa,b, Kyung-Mo Songc, Yun-Sang Choic, You-Jin Jeona( ), Min-Cheol Kangc( )
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea
Department of Biotechnology, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP) 60170, Sri Lanka
Research Group of Food Processing, Korea Food Research Institute, 245, Wanju 55365, Republic of Korea

Peer review under responsibility of Tsinghua University Press.

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Abstract

Low molecular weight polysaccharides can be isolated from Sargassum thunbergii (LMPST) and in vitro experiments were conducted to evaluate the inhibitory effects on lipids. Two natures of LMPST were attained from S. thunbergii and appraised their LMPST on palmitic acid (PA) induced lipid accretion in HepG2, and 3T3-L1 cells. LMPST treatment lessened lipid deposition and intracellular free fatty acid and triglyceride intensities in PA-treated above mentioned cells. The mechanistic study publicized that LMPST2 significantly suppressed adipogenesis and stimulated the PA-treated 3T3-L1 cells occupied in the lipolysis pathway. Furthermore, in PA-treated HepG2 cells, the free fatty acid oxidation was significantly increased by LMPST2. Given these constructive properties of LMPST2 from S. thunbergii, is a potential candidate for diminishing the intracellular lipids, and for a therapeutic agent in those conditions.

Keywords

ObesityLipid accumulationLow molecular weight polysaccharideLiver steatosisSargassum thunbergii

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Food Science and Human Wellness
Volume 13 Issue 4,
July 2024
Pages 2244-2252
DOI: 10.26599/FSHW.2022.9250187
Cite this article:
Lee H-G, Nagahawatta D, Kurera M, et al. Effects of low molecular weight polysaccharide from Sargassum thunbergii against palmitic acid-induced intracellular lipid accumulation in 3T3-L1 adipocyte and HepG2 cells. Food Science and Human Wellness, 2024, 13(4): 2244-2252. https://doi.org/10.26599/FSHW.2022.9250187

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Received: 19 December 2022
Revised: 10 January 2023
Accepted: 27 January 2023
Published: 20 May 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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