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Research Article | Open Access

Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway

Young-Jin Choia,b,c,, Meiqi Fand, Nishala Erandi Wedamullaa,b,c,e, Yujiao Tangf, Eun-Kyung Kimg,h( )
Department of Food Science and Nutrition, Dong-A University, Busan 49315, Republic of Korea
Center for Silver-targeted Biomaterials, Brain Busan 21 Plus program, Dong-A University, Busan 49315, Republic of Korea
Department of Health Sciences, the Graduate School of Dong-A University, Busan 49315, Republic of Korea
Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Republic of Korea
Department of Export Agriculture, Faculty of Animal Science and Export Agriculture, Uva Wellassa University, Badulla 90000, Sri Lanka
School of Bio-Science and Food Engineering, Changchun University of Science and Technology, Changchun 130600, China
Nutritional Education Major, Graduate School of Education, Dong-A University, Busan 49315, Republic of Korea
Nutrinomics Lab. Co., Ltd., Busan 49315, Republic of Korea

Peer review under responsibility of Tsinghua University Press.

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Abstract

We evaluated the effect of isoquercetin (quercetin-O-3-glucoside-quercetin, IQ) as a functional component of Abeliophyllum disistichum Nakai ethanol extract (ADLE) on prostate cell proliferation and apoptosis and its effects on the IGF-1/PI3K/Akt/mTOR pathway in benign prostatic hyperplasia (BPH). Metabolites in ADLE were analyzed using UHPLC-qTOF-MS and HPLC. IQ was orally administered (1 or 10 mg/kg) to a testosterone propionate-induced BPH rat model, and its effects on the prostate weight were evaluated. The effect of IQ on androgen receptor (AR) signaling was analyzed in LNCaP cells. Whether IGF-1 and IQ affect the IGF-1/PI3K/Akt/mTOR pathway in BPH-1 cells was also examined. The metabolites in ADLE were identified and quantified, which confirmed that ADLE contained abundant IQ (20.88 mg/g). IQ significantly reduced the prostate size in a concentration-dependent manner in a BPH rat model, and significantly decreased the expression of AR signaling factors in the rat prostate tissue and LNCaP cells in a concentration-dependent manner. IQ also inhibited the PI3K/AKT/mTOR pathway activated by IGF-1 treatment in BPH-1 cells. In BPH-1 cells, IQ led to G0/G1 arrest and suppressed the expression of proliferation factors while inducing apoptosis. Thus, IQ shows potential for use as a pharmaceutical and nutraceutical for BPH.

Keywords

PI3K/Akt/mtor pathwayIsoquercetinBenign prostatic hyperplasiaAndrogen receptor signaling

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Food Science and Human Wellness
Volume 13 Issue 3,
May 2024
Pages 1698-1710
DOI: 10.26599/FSHW.2022.9250216
Cite this article:
Choi Y-J, Fan M, Wedamulla NE, et al. Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway. Food Science and Human Wellness, 2024, 13(3): 1698-1710. https://doi.org/10.26599/FSHW.2022.9250216

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Received: 04 August 2022
Revised: 04 November 2022
Accepted: 06 December 2022
Published: 08 February 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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