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Open Access

Carbonic anhydrase 2 mediates anti-obesity effects of black tea as thermogenic activator

Peng Maa, Jie Xiaob, Biyu Houa, Ping Hec, Xinyu Yangd, Yisa Wanga,e, Zijing Wanga, Tianshu Xua, Xiuying Yanga, Xuan Zhuf, Shasha Xiangf, Song Lig, Guanhua Dua( ), Jian Yingb,h( ), Guifen Qianga( )
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College and Beijing Key Laboratory of Drug Target and Screening Research, Beijing 100050, China
COFCO Nutrition and Health Research Institute, Beijing 102209, China
Department of Pharmacy, The Eighth Affi liated Hospital, Sun Yat-sen University, Shenzhen 518000, China
Faculty of Biology, Medicine and Health Sciences, The University of Manchester, Manchester M13 9PL, United Kingdom
Changchun University of Chinese Medicine, Changchun 130117, China
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
China Tea Technology (Beijing) Co., Ltd., Beijing 102209, China
Beijing Key Laboratory of Nutrition, Health and Food Safety, Beijing 102209, China

Peer review under responsibility of Tsinghua University Press.

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Abstract

Obesity is a metabolic disorder due to over-accumulation of adipose tissue and ultimately becomes a “disease”. Brown adipose tissue (BAT) thermogenesis and white adipose tissue (WAT) browning emerge as a potential strategy of anti-obesity by dissipating energy as heat. However, drugs based on adipose tissue thermogenesis have not been successfully approved yet. In current study, we found that black tea extract (BTE) obtained by patent-authorized manufacturing process prevented body weight gain as novel thermogenic activator with reduction of adiposity, improvement of adipose distribution, and glucose metabolism improvement in diet-induced obesity mice. Mechanismly, anti-obesity effect of BTE depends on promoting BAT thermogenesis and WAT browning with upregulation of uncoupling protein 1 (UCP1), especially visceral adipose tissue (VAT) with browning resistance. Specifically, utilizing in silico approach of network pharmacology and molecular docking, we identified carbonic anhydrase 2 (CA2) in nitrogen metabolism as anti-obesity target of BTE and further elucidated that protein kinase B (AKT) signaling pathway linked CA2 and UCP1. Meanwhile gut microbiota regulation may prompt the CA2-dependent thermogenesis activation. Our findings demonstrated anti-obesity effect of BTE as thermogenic activator through CA2-mediated BAT thermogenesis and WAT browning via CA2-AKT-UCP1 signaling pathway, which could be developed as promising anti-obesity agent with good safety and efficacy.

Keywords

ObesityBlack teaThermogenesisBrowningAdipose tissue

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Food Science and Human Wellness
Volume 13 Issue 5,
September 2024
Pages 2917-2936
DOI: 10.26599/FSHW.2022.9250236
Cite this article:
Ma P, Xiao J, Hou B, et al. Carbonic anhydrase 2 mediates anti-obesity effects of black tea as thermogenic activator. Food Science and Human Wellness, 2024, 13(5): 2917-2936. https://doi.org/10.26599/FSHW.2022.9250236

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Received: 29 January 2023
Revised: 09 February 2023
Accepted: 27 February 2023
Published: 10 October 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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