Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in lipopolysaccharide-induced RAW264.7 cell model

Xiankang Fan; Laidi Zhang; Yangying Sun; Changyu Zhou; Qiang Xia; Lihui Du; Zhen Wu; Daodong Pan

doi:10.26599/FSHW.2023.9250041

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Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in lipopolysaccharide-induced RAW264.7 cell model

Xiankang Fan^{^a^,^b^,¹}, Laidi Zhang^{^a^,^b^,¹}, Yangying Sun^{^a^,^b}, Changyu Zhou^{^a^,^b}, Qiang Xia^{^a^,^b}, Lihui Du^{^a^,^b}, Zhen Wu^{^a}

(), Daodong Pan^{^a^,^b}()

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China

Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China

¹ Co-first author.

Peer review under responsibility of Tsinghua University Press.

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Abstract

In this study, 10 novel anti-inflammatory peptides were identified from duck liver, and their molecular mechanism was demonstrated based on machine learning and molecular docking. Using Sephadex G-15 gel chromatography separation, reversed-phase high-performance liquid chromatography purification, liquid chromatography-tandem mass spectrometry identification, and BIOPEP database comparison, 10 novel anti-inflammatory peptides were initially found. Their splendid angiotensin-converting enzyme (ACE) inhibition and anti-inflammatory properties were confirmed by machine learning. With binding energies less than –20.93 kJ/mol, molecular docking revealed that they could efficiently bind to the active pockets of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), cyclooxygenase 2 (COX-2), and nuclear factor κB (NF-κB) proteins with efficiency, indicating that the compounds can spontaneously form complexes through hydrogen bonding and hydrophobic interactions with the protein binding pockets. In the lipopolysaccharide-induced RAW264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (TNF-α, IL-6, COX-2, and NF-κB) were significantly inhibited by these peptides. We concluded it might be due to their anti-inflammatory effects by inhibiting the protein phosphorylation of inhibitor of NF-κB (IκBα) in the cytoplasm and preventing the translocation of NF-κB p65 in the cytoplasm to the nucleus, thereby regulating the NF-κB signaling pathway. This study is essential for the screening of anti-inflammatory peptides and the investigation of the mechanism of action.

Keywords

Peptides Machine learning Molecular docking Inflammation Liquid chromatography-tandem mass spectrometry

References

[1]

L. Xu, Q. Xia, J.X. Cao, et al., Ultrasonic effects on the headspace volatilome and protein isolate microstructure of duck liver, as well as their potential correlation mechanism, Ultrason. Sonochem. 71 (2020) 105358. https://doi.org/10.1016/j.ultsonch.2020.105358.