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Research Article | Open Access | Just Accepted

Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in LPS-induced RAW 264.7 cell model

Xiankang Fana,b,1Laidi Zhanga,b,1Yangying Suna,bChangyu Zhoua,bQiang Xiaa,bLihui Dua,bZhen Wua ( )Daodong Pana,b( )

a State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China

b Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China

1 Co-first author: Xiankang Fan, Laidi Zhang

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Abstract

In this study, 10 novel anti-inflammatory peptides were identified from duck liver, and their molecular mechanism was demonstrated based on machine learning and molecular docking. Using Sephadex G-15 gel chromatography separation, reversed-phase high-performance liquid chromatography purification, liquid chromatography-tandem mass spectrometry identification, and Biopep database comparison, 10 novel anti-inflammatory peptides were initially found. Their splendid ACE inhibition and anti-inflammatory properties were confirmed by machine learning. With binding energies less than -5.0 kcal/mol, molecular docking revealed that they could efficiently bind to the active pockets of TNF-, IL-6, COX-2, and NF-B proteins with efficiency, indicating that the compounds can spontaneously form complexes through hydrogen bonding and hydrophobic interactions with the protein binding pockets. In the LPS-induced RAW 264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (TNF-α, IL-6, COX-2, and NF-κB) were significantly inhibited by these peptides. We concluded it might be due to their anti-inflammatory effects by inhibiting the protein phosphorylation of IκBα in the cytoplasm and preventing the translocation of NF-κB p65 in the cytoplasm to the nucleus, thereby regulating the NF-κB signaling pathway. This study is essential for the screening of anti-inflammatory peptides and the investigation of the mechanism of action.

Food Science and Human Wellness
Cite this article:
Fan X, Zhang L, Sun Y, et al. Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in LPS-induced RAW 264.7 cell model. Food Science and Human Wellness, 2024, https://doi.org/10.26599/FSHW.2023.9250041

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Received: 21 February 2023
Revised: 30 March 2023
Accepted: 11 April 2023
Available online: 26 February 2024

© 2024 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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