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Identification and analysis of immunological activity of two isoforms of tropomyosin in Alectryonella plicatula

Shiqiang Yanga, Yexin Chena, Fei Huana, Xinrong Hea, Xiao Yuna, Hong Liua, Guixia Chenb, Guangming Liua,c ()
College of Ocean Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen 361021, China
Women and Children’s Hospital Affi liated to Xiamen University, Xiamen 361003, China
College of Marine Biology, Xiamen Ocean Vocational College, Xiamen, Fujian 361100, China

Peer review under responsibility of Tsinghua University Press.

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Highlights

• Two tropomyosin isoforms were identified as allergen in Alectryonella plicatula.

• The immunological activity of TM-β were significantly higher than that of TM-α.

• High α-helix content is the key to high immunological activity.

• The amino acids of TM-β were more similar to the epitopes of shellfish TMs.

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Abstract

Oyster, as a common aquatic food, play an important role in shellfish allergy. In this study, 2 tropomyosin (TM) isoforms TM-α and TM-β (TM-α/-β) in Alectryonella plicatula were identified. The sequences of 852 bp encoding 284 amino acids of TM-α/-β and 2 recombinant proteins were obtained, respectively. There were 12 amino acid differences between TM-α/-β. The results of immunological experiments indicated that TM-β had stronger immunobinding activity and immunoreactivity than those of TM-α. Structural analysis showed that TM-β had more α-helix and higher surface hydrophobicity than TM-α. Sequences and epitopes alignment with shellfish TMs revealed that amino acids of TM-β were more frequently recognized as IgE epitopes in other shellfish TMs than TM-α. Differences in structure and sequence account for the higher immunological activity of TM-β compared to TM-α. These findings provide a theoretical basis for enriching the understanding of shellfish TM and accurate diagnosis of allergic components.

Keywords

Alectryonella plicatulaAllergenImmunological activityIsoformsTropomyosin

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Food Science and Human Wellness
Volume 13 Issue 6,
November 2024
Pages 3697-3707
DOI: 10.26599/FSHW.2023.9250050
Cite this article:
Yang S, Chen Y, Huan F, et al. Identification and analysis of immunological activity of two isoforms of tropomyosin in Alectryonella plicatula. Food Science and Human Wellness, 2024, 13(6): 3697-3707. https://doi.org/10.26599/FSHW.2023.9250050
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