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Lactobacillus acidophilus FCQHC4L1 strengthens the intestinal mucus barrier and inhibits endoplasmic reticulum stress

Yuli Qia,b, Leilei Yua,b, Fengwei Tiana,b, Wei Chena,b,c, Xiaoming Liua,b(), Qixiao Zhaia,b()
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Highlights

· Dead bacteria or CM of different LAB strains affected Muc2 gene expression differently.

· CM of L. acidophilus FCQHC4L1 significantly increased the transcription of MUC2.

· FCQHC4L1 strengthened the intestinal mucus barrier in DSS-induced IBD mice.

· FCQHC4L1 significantly inhibited DSS-induced colitis development.

· CM of FCQHC4L1 inhibited ER stress and ameliorated the abnormal expression of MUC2.

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Abstract

Mucin 2 (MUC2) is a critical component of the intestinal mucus barrier. Lactic acid bacteria (LAB) strains can improve mucosal homeostasis. In this study, we determined the expression of Muc2 induced by dead bacteria and cell-free conditioned medium (CM) of 50 LAB strains in the human goblet cell line, LS174T. Dead bacteria or CM of LAB affected the Muc2 expression in a species- and strain-specific manner under homeostasis. Next, LAB strains with different regulatory abilities were selected, gavaged into mice, and exposed to dextran sodium sulfate (DSS) after 1 week. Different LAB strains inhibited intestinal injury to different degrees, with Lactobacillus acidophilus FCQHC4L1 exerting the most potent effect. FCQHC4L1 significantly decreased the secretion of pro-inflammatory factors, promoted the expression and secretion of mucin, and inhibited colitis development. This strain also regulated the gut microbiota and increased the secretion of butyric acid. Moreover, CM of FCQHC4L1 inhibited endoplasmic reticulum (ER) stress and ameliorated the abnormal expression of MUC2 by suppressing the activation of the GRP78/ATF6 and GRP78/IRE1/XBP1 signaling pathways. Our results highlight the potential of FCQHC4L1 as a therapeutic agent for strengthening the mucus barrier and improving the gut health.

Keywords

MucinProbioticLactobacillus acidophilusGut microbiotaEndoplasmic reticulum (ER) stress

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Food Science and Human Wellness
Volume 14 Issue 1,
January 2025
Article number: 9250005
DOI: 10.26599/FSHW.2024.9250005
Cite this article:
Qi Y, Yu L, Tian F, et al. Lactobacillus acidophilus FCQHC4L1 strengthens the intestinal mucus barrier and inhibits endoplasmic reticulum stress. Food Science and Human Wellness, 2025, 14(1): 9250005. https://doi.org/10.26599/FSHW.2024.9250005
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