<i>Dendrobium officinale</i> polysaccharide attenuates type 2 diabetes in mice model by modulating gut microbiota and alleviating intestinal mucosal barrier damage

Xiaoxia Chen; Chun Chen; Changyang Ma; Wenyi Kang; Junlin Wu; Xiong Fu

doi:10.26599/FSHW.2024.9250007

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Open Access

Dendrobium officinale polysaccharide attenuates type 2 diabetes in mice model by modulating gut microbiota and alleviating intestinal mucosal barrier damage

Xiaoxia Chen^{^a}, Chun Chen^{^a^,^b^,^c^,^d}(), Changyang Ma^{^e}, Wenyi Kang^{^e}, Junlin Wu^{^f}(), Xiong Fu^{^a^,^b^,^c^,^d}

Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China

SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China

Guangzhou Institute of Modern Industrial Technology, Guangzhou 511458, China

Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, China

National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China

Guangzhou Wondfo Health Science and Technology Co., Ltd., Guangzhou 510640, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Abstract

The purpose of this study was to investigate the hypoglycemic effect and mechanism of Dendrobium officinale polysaccharide (DOP) on type 2 diabetes mellitus (T2DM) mice established by high-fat diet and streptozotocin. The results showed that DOP improved glycolipid metabolism and serum inflammation levels, and inhibited intestinal-derived lipopolysaccharide (LPS) translocation, suggesting that inhibiting LPS-mediated intestinal barrier damage may be a key target for DOP to alleviate T2DM. Interestingly, the study found that DOP reduced intestinal inflammation and oxidative stress levels, significantly up-regulated the mRNA expression of tight junction proteins Claudin-1, Occludin and zonula occluden-1 (ZO-1), and ameliorated intestinal epithelial damage. In addition, DOP strongly inhibited the intestinal pathogenic bacteria and LPS-producing bacteria Helicobacter, Enterococcus and Desulfovibrio with a reduction rate of 95%, 73% and 9%, respectively, and promoted the proliferation of anti-inflammatory bacteria Bifidobacterium and Lactobacillus by 139% and 8%, respectively. Taken together, the hypoglycemic effect of DOP was related to the protection of intestinal mucosal barrier, and its underlying mechanism lied in its excellent anti-inflammatory and gut microbiota-modulatory effects, providing a theoretical basis for developing DOP as a novel prebiotic in functional food for diabetes.

Keywords

Dendrobium officinale polysaccharide Hypoglycemic Mechanism Lipopolysaccharide Gut microbiota

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References

[1]

L. Xu, Y. Li, Y. Dai, et al., Natural products for the treatment of type 2 diabetes mellitus: pharmacology and mechanisms, Pharmacol. Res. 130 (2018) 451-465. https://doi.org/10.1016/j.phrs.2018.01.015.

Variable 1	Variable 2	r	P
Note: , , ** indicate significant correlation (P < 0.05, P < 0.01, P < 0.001, respectively).
LPS	FBG	0.611	0.0155*
LPS	GSP	0.658	0.0077**
LPS	AUC_OGTT	0.707	0.0030**
LPS	FINS	0.699	0.0037**
LPS	HOMA-IR	0.789	0.0005***
LPS	TC	0.686	0.0048**
LPS	TG	0.650	0.0087**
LPS	LDL-C	0.686	0.0047**
LPS	HDL-C	−0.574	0.0253*
LPS	TNF-α	0.814	0.0002***
LPS	IL-6	0.572	0.0257*
LPS	IL-1β	0.803	0.0003***
LPS	IL-10	−0.277	0.3175