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2’-Fucosyllactose modulates the function of intestinal microbiota to reduce intestinal permeability in mice colonized by feces from healthy infants

Qingxue Chena,b, Liu Yanga,b, Fangqin Xianga,b, Ignatius Man-Yau Szetoc,d, Yalu Yand, Biao Liud, Jinju Chenga, Lu Liua,b, Bailiang Lia,b (), Sufang Duanc,d()
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China
College of Food Science, Northeast Agricultural University, Harbin 150030, China
National Center of Technology Innovation for Dairy, Hohhot 010110, China
Inner Mongolia Yili Industrial Group, Co., Ltd., Yili Maternal and Infant Nutrition Institute (YMINI), Beijing 100070, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Highlights

• 2′-Fucosyllactose (2′-FL) is a naturally occurring prebiotic in human milk.

• The human microbiota-associated (HMA) mouse model is a cutting-edge and effective means of studying the composition of infant foods.

• 2′-FL modulates the intestinal microbial composition and metabolic function.

• The improvement of intestinal microenvironment promotes intestinal permeability.

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Abstract

2’-Fucosyllactose (2’-FL) shows the potential to support intestinal health as a natural prebiotic that bridges the gap between infant formula feeding and breastfeeding. However, the effect and mechanism of 2’-FL in improving intestinal permeability are not clear. In this study, we constructed human microbiota-associated (HMA) mouse models by colonizing healthy infant feces in mice with antibiotic-depleted intestinal microbiota. The protective effect of 2’-FL on the intestinal permeability was explored using the HMA mouse models, and the combination of metagenomics was used to analyze the possible mechanisms by which the microorganisms reduced the intestinal permeability. The results showed that 2’-FL decreased the concentration of markers of intestinal permeability (enterotoxin and diamine oxidase (DAO)) and increased the expression levels of tight junctions (occludin and claudin). Metagenomics revealed the enrichment of Bifidobacterium and increased the expression of glycoside hydrolases (GHs), including GH31, GH28, and GH5. In conclusion, 2’-FL strengthened intestinal permeability function by improving microbiota composition to control the translocation of harmful substance.

Keywords

2’-FucosyllactoseIntestinal permeabilityIntestinal microbiotaBifidobacterium

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Food Science and Human Wellness
Volume 14 Issue 1,
January 2025
Article number: 9250021
DOI: 10.26599/FSHW.2024.9250021
Cite this article:
Chen Q, Yang L, Xiang F, et al. 2’-Fucosyllactose modulates the function of intestinal microbiota to reduce intestinal permeability in mice colonized by feces from healthy infants. Food Science and Human Wellness, 2025, 14(1): 9250021. https://doi.org/10.26599/FSHW.2024.9250021
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