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Open Access

Bifidobacterium breve CCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption

Bowen Lia,b, Mengfan Dinga,b, Xiaoming Liua,b, Haiqin Chena,b, Jianxin Zhaoa,b,c, Ross R. Paulc,d, Catherine Stantonc,d,e, Shilong Jiangf,g, Wei Chena,b,h, Bo Yanga,b,c( )
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, China
APC Microbiome Ireland, University College Cork, Cork T12 K8AF, Ireland
Teagasc Food Research Centre, Cork P61 C996, Ireland
Nutrition and Metabolism Research Division, Innovation Center, Heilongjiang Feihe Dairy Co., Ltd., Beijing 100015, China
PKUHSC-China Feihe Joint Research Institute of Nutrition and Healthy Lifespan Development, Beijing 100083, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Abstract

This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice. Newborn BALB/c mice were assigned to the control group (administration saline) and the CCFM1078 group (administration B. breve CCFM1078, 3 × 109 CFU/day) in 3-, 4-, and 5-week tests. All the groups have male and female distinctions. Our findings demonstrate that B. breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites (hexadecanamide, linoleoyl ethanolamide, and palmitoyl ethanolamide), the genes and proteins expression related to the growth hormone (GH)/insulin-like growth factors-1 (IGF-1) axis and Gs/PKA/CREB signaling pathways, as well as downstream osteogenic and osteoclastic differentiation factors. The effects of B. breve CCFM1078 were different with age and gender dependent. This finding suggests B. breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.

Keywords

Bifidobacterium breveBone developmentGut microbiotaGH/IGF-1 axisGs/PKA/CREB signaling pathway

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Food Science and Human Wellness
Volume 14 Issue 1,
January 2025
Article number: 9250023
DOI: 10.26599/FSHW.2024.9250023
Cite this article:
Li B, Ding M, Liu X, et al. Bifidobacterium breve CCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption. Food Science and Human Wellness, 2025, 14(1): 9250023. https://doi.org/10.26599/FSHW.2024.9250023

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Received: 05 May 2023
Revised: 09 May 2023
Accepted: 17 May 2023
Published: 14 February 2025
© 2025 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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