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Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice

Mengfan Dinga,b, Bo Yanga,b,c(), Bowen Lia,b, Haiqin Chena,b, Renqiang Yud(), Ross R. Paulc,e, Catherine Stantonc,e,f, Shilong Jiangg,h, Jianxin Zhaoa,b,c, Wei Chena,b,i
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, China
Department of Neonatology, Wuxi Maternity and Child Health Care Hospital, Affiliated Women’s Hospital of Jiangnan University, Wuxi 214122, China
APC Microbiome Ireland, University College Cork, Cork T12 R229, Ireland
Teagasc Food Research Centre, Cork P61 C996, Ireland
Nutrition and Metabolism Research Division, Innovation Center, Heilongjiang Feihe Dairy Co., Ltd., Beijing 100015, China
PKUHSC-China Feihe Joint Research Institute of Nutrition and Healthy Lifespan Development, Beijing 100083, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Abstract

Bifidobacterium longum subsp. infantis is a commensal bacterium that predominates in the infant gut, playing a critical role in both preventing foreign infections and facilitating immune development. This study aimed to explore the effects of B. longum subsp. infantis supplementation on interferon-beta (IFN-β) secretion and intestinal barrier improvement in growing mice. Female and male mice were orally administered either saline or B. longum subsp. infantis CCFM1269 or I5TI (1 × 109 CFU/mice per day, n = 8) from 1-week-age until 3-, 4-, and 5-week-age. RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2’-5’ oligoadenylate synthetase (OAS). Additionally, CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway. However, this effect was not observed in 4- and 5-week-old mice. Furthermore, both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-, 4-, and 5-week-old mice. In summary, the results of this study suggested that B. longum subsp. infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.

Keywords

Bifidobacterium longum subsp. infantisIntestinal barrierinterferon-beta (IFN-β) secretionAge dependence

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Food Science and Human Wellness
Volume 14 Issue 1,
January 2025
Article number: 9250025
DOI: 10.26599/FSHW.2024.9250025
Cite this article:
Ding M, Yang B, Li B, et al. Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice. Food Science and Human Wellness, 2025, 14(1): 9250025. https://doi.org/10.26599/FSHW.2024.9250025
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