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Mitochondria-targeted nanoparticles for encapsulating astaxanthin: exploring the reason for alleviating oxidative damage

Yannan Chena,b,c, Siyuan Feia,b,c, Lijuan Zhanga,b,c, Mingqian Tana,b,c()
Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China
Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Highlights

• Mitochondria-targeted nanocarriers could specifically accumulate in cell mitochondria.

• Mitochondria-targeted astaxanthin nanocarriers could alleviate H2O2-induced cell damage.

• Mitochondria-targeted astaxanthin nanocarriers rescued the H2O2-induced metabolic disorder, and further alleviated oxidative damage.

Abstract

Oxidative stress is considered as a critical factor in the process of pathological diseases, and mitochondria are considered as vital target organelles for disease intervention. The purpose of this study was aimed to evaluate the antioxidant efficacy of mitochondria-targeted astaxanthin nanoparticle on hydrogen peroxide-induced oxidative damage. As expected, mitochondria-targeted nanoparticle showed excellent mitochondria co-localization ability with higher Pearson’s correlation coefficient (r = 0.88). In vitro experiments suggested that the mitochondria-targeted astaxanthin nanoparticle could promote cell viability and increase antioxidant-related enzyme activities. Simultaneously, metabolomics analysis indicated that mitochondria-targeted astaxanthin nanoparticle could alleviate oxidative stress by regulating amino acid metabolism and energy metabolism. Altogether, all these results strongly confirmed the mitochondria-targeted strategy for astaxanthin delivery could relieve oxidative stress and had great promise in the application of disease intervention.

Keywords

Oxidative stressMitochondria-targetedAstaxanthinAntioxidantMetabolism

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References

[1]

J. Qu, F. Ke, Z. Liu, et al., Uncovering the mechanisms of dandelion against triple-negative breast cancer using a combined network pharmacology, molecular pharmacology and metabolomics approach, Phytomedicine 99 (2022) 153986. 10.1016/j.phymed.2022.153986.

Crossref Google Scholar
[2]

Y. Sun, Y. Wu, Z. Wang, et al., Dandelion extract alleviated lipopolysaccharide-induced oxidative stress through the Nrf2 pathway in bovine mammary epithelial cells, Toxins 12(8) (2020) 496. https://doi.org/10.3390/toxins12080496.

Crossref Google Scholar
[3]

N. Kamimura, A.M. Wolf, T. Yokota, et al., Transgenic type 2 diabetes mouse models for in vivo redox measurement of hepatic mitochondrial oxidative stress, Biochim. Biophys. Acta Gen. Subj. 1867(3) (2023) 130302. https://doi.org/10.1016/j.bbagen.2022.130302.

Crossref Google Scholar
[4]

P. Ghosh, T. Dey, R. Majumder, et al., Insights into the antioxidative mechanisms of melatonin in ameliorating chromium-induced oxidative stress-mediated hepatic and renal tissue injuries in male Wistar rats, Food Chem. Toxicol. 173 (2023) 113630. https://doi.org/10.1016/j.fct.2023.113630.

Crossref Google Scholar
[5]

B. Lis, A. Rolnik, D. Jedrejek, et al., Dandelion (Taraxacum officinale L.) root components exhibit anti-oxidative and antiplatelet action in an in vitro study, J. Funct. Foods 59 (2019) 16-24. https://doi.org/10.1016/j.jff.2019.05.019.

Crossref Google Scholar
[6]

S. Oh, Y.J. Kim, E.K. Lee, et al., Antioxidative effects of ascorbic acid and astaxanthin on ARPE-19 cells in an oxidative stress model, Antioxidants 9(9) (2020) 833. https://doi.org/10.3390/antiox9090833.

Crossref Google Scholar
[7]

X. Zhang, X. Zhao, S. Tie, et al, A smart cauliflower-like carrier for astaxanthin delivery to relieve colon inflammation, J. Control. Release 342 (2022) 372-387. https://doi.org/10.1016/j.jconrel.2022.01.014.

Crossref Google Scholar
[8]

Y. Chen, W. Su, S. Tie, et al., Advances of astaxanthin-based delivery systems for precision nutrition, Trends Food Sci. Tech. 127 (2022) 63-73. https://doi.org/10.1016/j.tifs.2022.07.007.

Crossref Google Scholar
[9]

X. Li, T. Matsumoto, M. Takuwa, et al., Protective effects of astaxanthin supplementation against ultraviolet-induced photoaging in hairless mice. Biomedicines 8(2) (2020) 18. https://doi.org/10.3390/biomedicines8020018.

Crossref Google Scholar
[10]

T. Zhao, X. Yan, L. Sun, et al., Research progress on extraction, biological activities and delivery systems of natural astaxanthin, Trends Food Sci. Tech. 91 (2019) 354-361. https://doi.org/10.1016/j.tifs.2019.07.014.

Crossref Google Scholar
[11]

Y. Tian, H. Che, J. Yang, et al., Astaxanthin alleviates aflatoxin B1-induced oxidative stress and apoptosis in IPEC-J2 cells via the Nrf2 signaling pathway, Toxins 15(3) (2023) 232. https://doi.org/10.3390/toxins15030232.

Crossref Google Scholar
[12]

X. Lin, H. Bo, J. Gu, et al., Astaxanthin, a carotenoid antioxidant, pretreatment alleviates cognitive deficits in aircraft noised mice by attenuating inflammatory and oxidative damage to the gut, heart and hippocampus, Biomed. Pharmacother. 148 (2022) 112777.

Crossref Google Scholar
[13]

D. Özbeyli, E.B. Gurler, H. Buzcu, et al., Astaxanthin alleviates oxidative damage in acute pancreatitis via direct antioxidant mechanisms, Turk. J. Gastroenterol. 31(10) (2020) 706-712. https://doi.org/10.5152/tjg.2020.19520.

Crossref Google Scholar
[14]

S. Fakhri, F. Abbaszadeh, L. Dargahi, et al., Astaxanthin: a mechanistic review on its biological activities and health benefits, Pharmacol. Res. 136 (2018) 1-20. https://doi.org/10.1016/j.phrs.2018.08.012.

Crossref Google Scholar
[15]

C. Liu, S. Zhang, D.J. McClements, et al., Design of astaxanthin-loaded core-shell nanoparticles consisting of chitosan oligosaccharides and poly(lactic-co-glycolic acid): enhancement of water solubility, stability, and bioavailability, J. Agric. Food Chem. 67(18) (2019) 5113-5121. https://doi.org/10.1021/acs.jafc.8b06963.

Crossref Google Scholar
[16]

Q. Zhang, Y. Zhou, W. Yue, et al, Nanostructures of protein-polysaccharide complexes or conjugates for encapsulation of bioactive compounds, Trends Food Sci. Tech. 109 (2021) 169-196. https://doi.org/10.1016/j.tifs.2021.01.026.

Crossref Google Scholar
[17]

T. Zhang, J. Xu, J. Chen, et al., Protein nanoparticles for Pickering emulsions: a comprehensive review on their shapes, preparation methods, and modification methods, Trends Food Sci. Tech. 113 (2021) 26-41. https://doi.org/10.1016/j.tifs.2021.04.054.

Crossref Google Scholar
[18]

D.J. McClements, B. Ozturk, Utilization of nanotechnology to improve the application and bioavailability of phytochemicals derived from waste streams, J. Agric. Food Chem. 70(23) (2021) 6884-6900. https://doi.org/10.1021/acs.jafc.1c03020.

Crossref Google Scholar
[19]

L. Zhang, C. Zhou, X. Na, et al., High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery, Food Funct. 12(23) (2021) 11872-11882. https://doi.org/10.1039/d1fo02117f.

Crossref Google Scholar
[20]

D. Li, M. Yang, H. Xu, et al., Nanoparticles for oral delivery: targeted therapy for inflammatory bowel disease, J. Mater. Chem. B 10 (2022) 5853-5872. https://doi.org/10.1039/d2tb01190e.

Crossref Google Scholar
[21]

C. Fang, G. Xiao, T. Wang, et al., Emerging nano-/biotechnology drives oncolytic virus-activated and combined cancer immunotherapy, Research 6 (2023) 0108. https://doi.org/10.34133/research.0108.

Crossref Google Scholar
[22]

M. Sztretye, B. Dienes, M. Gonczi, et al., Astaxanthin: a potential mitochondrial-targeted antioxidant treatment in diseases and with aging, Oxid. Med. Cell Longev. 2019 (2019) 3849692. https://doi.org/10.1155/2019/3849692.

Crossref Google Scholar
[23]

I. Hori, H. Harashima, Y. Yamada, Development of a mitochondrial targeting lipid nanoparticle encapsulating berberine, Int. J. Mol. Sci. 24(2) (2023) 903. https://doi.org/10.3390/ijms24020903.

Crossref Google Scholar
[24]

Z. Zeng, C. Fang, Y. Zhang, et al., Mitochondria-targeted nanocarriers promote highly efficient cancer therapy: a review, Front. Bioeng. Biotechnol. 9 (2021) 784602. https://doi.org/10.3389/fbioe.2021.784602.

Crossref Google Scholar
[25]

Y. Chen, S. Tie, X. Zhang, et al., Preparation and characterization of glycosylated protein nanoparticles for astaxanthin mitochondria targeting delivery, Food Funct. 12(17) (2021) 7718-7727. https://doi.org/10.1039/d1fo01751a.

Crossref Google Scholar
[26]

Z. Hua, X. Zhang, X. Zhao, et al., Hepatic-targeted delivery of astaxanthin for enhanced scavenging free radical scavenge and preventing mitochondrial depolarization, Food Chem. 406 (2023) 135036. https://doi.org/10.1016/j.foodchem.2022.135036.

Crossref Google Scholar
[27]

Y. Chen, W. Su, S. Tie, et al., Orally deliverable sequence-targeted astaxanthin nanoparticles for colitis alleviation, Biomaterials 293 (2022) 121976. https://doi.org/10.1016/j.biomaterials.2022.121976.

Crossref Google Scholar
[28]

Z. Qi, Q. Wang, S. Song, et al., Enhanced cytotoxicity of cadmium by a sulfated polysaccharide from abalone, J. Agric. Food Chem. 68(50) (2020) 14996-15004. https://doi.org/10.1021/acs.jafc.0c06399.

Crossref Google Scholar
[29]

S. Tie, W. Su, Y. Chen, et al., Dual targeting procyanidin nanoparticles with glutathione response for colitis treatment, Chem. Eng. J. 441 (2022) 136095. https://doi.org/10.1016/j.cej.2022.136095.

Crossref Google Scholar
[30]

X. Zhang, X. Zhao, S. Tie, et al., Ultrasonic self-emulsification nanocarriers for cellular enhanced astaxanthin delivery, J. Agric. Food Chem. 69(9) (2021) 2719-2728. https://doi.org/10.1021/acs.jafc.0c05983.

Crossref Google Scholar
[31]

M. Li, L. Dong, H. Du, et al., Potential mechanisms underlying the protective effects of Tricholoma matsutake singer peptides against LPS-induced inflammation in RAW264.7 macrophages, Food Chem. 353 (2021) 129452. https://doi.org/10.1016/j.foodchem.2021.129452.

Crossref Google Scholar
[32]

X. Zhao, A. Abulikemu, S. Lv, et al., Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective, Chemosphere 275 (2021) 129969. https://doi.org/10.1016/j.chemosphere.2021.129969.

Crossref Google Scholar
[33]

D. Li, X. Na, H. Wang, et al., The effects of carbon dots produced by the Maillard reaction on the HepG2 cell substance and energy metabolism, Food Funct. 11(7) (2020) 6487-6495. https://doi.org/10.1039/d0fo01350a.

Crossref Google Scholar
[34]

X.B. Li, J.D. Gu, Q.H. Zhou, Review of aerobic glycolysis and its key enzymes-new targets for lung cancer therapy, Thorac. Cancer 6(1) (2015) 17-24. https://doi.org/10.1111/1759-7714.12148.

Crossref Google Scholar
[35]

Z. Qi, Q. Wang, H. Wang, et al., Metallothionein attenuated arsenic-induced cytotoxicity: the underlying mechanism reflected by metabolomics and lipidomics, J. Agric. Food Chem. 69(18) (2021) 5372-5380. https://doi.org/10.1021/acs.jafc.1c00724.

Crossref Google Scholar
[36]

B. Yue, X. Zhang, W. Li, et al., Fluoride exposure altered metabolomic profile in rat serum, Chemosphere 258 (2020) 127387. https://doi.org/10.1016/j.chemosphere.2020.127387.

Crossref Google Scholar
[37]

B. Yu, A.H. Li, D. Muzny, et al., Association of rare loss-of-function alleles in HAL, serum histidine: levels and incident coronary heart disease, Circ. Cardiovasc. Genet. 8(2) (2015) 351-355. https://doi.org/10.1161/circgenetics.114.000697.

Crossref Google Scholar
[38]

S. Mei, X. Song, Y. Wang, et al., Studies on protection of astaxanthin from oxidative damage induced by H2O2 in RAW 264.7 cells based on 1H NMR metabolomics, J. Agric. Food Chem. 67(49) (2019) 13568-13576. https://doi.org/10.1021/acs.jafc.9b04587.

Crossref Google Scholar
[39]

W.N. Zhang, A.P. Li, Y.S. Qi, et al., Metabolomics coupled with system pharmacology reveal the protective effect of total flavonoids of Astragali Radix against adriamycin-induced rat nephropathy model, J. Pharm. Biomed. Anal. 158 (2018) 128-136. https://doi.org/10.1016/j.jpba.2018.05.045.

Crossref Google Scholar
[40]

B. Kalyanaraman, Teaching the basics of cancer metabolism: developing antitumor strategies by exploiting the differences between normal and cancer cell metabolism, Redox Biol. 12 (2017) 833-842. https://doi.org/10.1016/j.redox.2017.04.018.

Crossref Google Scholar
[41]

X. Hu, J.D. Chandler, S. Park, et al., Low-dose cadmium disrupts mitochondrial citric acid cycle and lipid metabolism in mouse lung, Free Radical Bio. Med. 131 (2019) 209-217. https://doi.org/10.1016/j.freeradbiomed.2018.12.005.

Crossref Google Scholar
[42]

X. Qu, H. Gao, J. Sun, et al., Identification of key metabolites during cisplatin-induced acute kidney injury using an HPLC-TOF/MS-based non-targeted urine and kidney metabolomics approach in rats, Toxicology 431 (2020) 152366. https://doi.org/10.1016/j.tox.2020.152366.

Crossref Google Scholar
[43]

L.C. Shum, N.S. White, B.N. Mills, et al., Energy metabolism in mesenchymal stem cells during osteogenic differentiation, Stem Cells Dev. 25(2) (2016) 114-122. https://doi.org/10.1089/scd.2015.0193.

Crossref Google Scholar
[44]

J. Gogola-Mruk, W. Marynowicz, K. Krawczyk, et al., Visfatin increases the invasive potential of ovarian granulosa tumor spheroids by reprogramming glucose metabolism, Reproduction 165(5) (2023) 521-531. https://doi.org/10.1530/rep-22-0443.

Crossref Google Scholar
[45]

L. Zhang, X. Na, B. Lai, et al., Effects of fluorescent carbon dots from the baked lamb on energy and lipid metabolism, Food Chem. 338 (2021) 127832. https://doi.org/10.1016/j.foodchem.2020.127832.

Crossref Google Scholar
[46]

T. Che, Y. Song, W. Su, et al., Hepatic parenchymal cell and mitochondrial-targeted astaxanthin nanocarriers for relief of high fat diet-induced nonalcoholic fatty liver disease, Food Funct. 14(6) (2023) 2908-2920. https://doi.org/10.1039/d2fo04036k.

Crossref Google Scholar
Food Science and Human Wellness
Volume 14 Issue 1,
January 2025
Article number: 9250080
DOI: 10.26599/FSHW.2024.9250080
Cite this article:
Chen Y, Fei S, Zhang L, et al. Mitochondria-targeted nanoparticles for encapsulating astaxanthin: exploring the reason for alleviating oxidative damage. Food Science and Human Wellness, 2025, 14(1): 9250080. https://doi.org/10.26599/FSHW.2024.9250080
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