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Article | Open Access

Antioxidant peptides from Lateolabrax japonicus to protect against oxidative stress injury in Drosophila melanogaster via biochemical and gut microbiota interaction assays

Chen Lia, Lichan Lia, Jing Chenga, Xu Chena, Mohamed A. Faragb, Xixi Caia(), Shaoyun Wanga()
College of Chemical Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China
Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt

Peer review under responsibility of Beijing Academy of Food Sciences.

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Highlights

• LPH exhibited excellent antioxidant stability in vitro and alleviated physiological indicators related to oxidative stress in vivo.

• LPH mitigated cell death and intestinal barrier dysfunction induced by oxidative stress.

• LPH improved gut microbiota structure in Drosophila induced by H2O2.

• The antioxidant mechanism of LPH is mediated via Nrf2 and mTOR pathways.

• The LPH showed potential as a functional food in antioxidant supplements.

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Abstract

Identification of natural substances with antioxidant properties is ongoing research for addressing issues related to oxidative stress especially attributed to environmental effects. Our previous study demonstrated that Lateolabrax japonicus peptides (LPH), rich in Glu, Gly, and hydrophobic amino acids, exhibited remarkable antioxidant activity in vitro, with though its action mechanism yet to be revealed. Therefore, to assess the in vivo antioxidative properties of LPH, we employed H2O2 to generate oxidative stress in Drosophila melanogaster model. Results indicated that LPH significantly prolonged the lifespan of Drosophila subjected to oxidative stress mostly mediated via LPH’s enhancement of the antioxidant defense system and intestinal functions. Antioxidant effects were manifested by a decrease in malondialdehyde (MDA) levels, elevated superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, decreased levels of reactive oxygen species (ROS) in intestinal epithelial cells, and the preservation of intestinal length. LPH effectively controlled the excessive proliferation and differentiation of oxidative stress-induced Drosophila intestinal stem cells. At the gene level, LPH upregulated the expression of antioxidant-related Nrf2 genes while concurrently downregulated mTOR expression level. Furthermore, high-throughput 16S rDNA sequencing revealed that the addition of LPH significantly influenced the diversity and abundance of the intestinal microbiota in H2O2-induced Drosophila. These findings provide a deeper understanding of the antioxidative mechanism of LPH, suggesting its potential applications in food industry and to be assessed using other in vivo oxidative stress models.

Keywords

Lateolabrax japonicusPeptidesOxidative stressIntestinal microbiotaDrosophilaAntioxidant properties

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Food Science and Human Wellness
Volume 14 Issue 7,
July 2025
Article number: 9250154
DOI: 10.26599/FSHW.2024.9250154
Cite this article:
Li C, Li L, Cheng J, et al. Antioxidant peptides from Lateolabrax japonicus to protect against oxidative stress injury in Drosophila melanogaster via biochemical and gut microbiota interaction assays. Food Science and Human Wellness, 2025, 14(7): 9250154. https://doi.org/10.26599/FSHW.2024.9250154
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