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Article | Open Access

Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota

Ruiyun Wua,b, Zixin Hanb, Zhenyu Wanga, Pinglan Lic, Nan Shangb()
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Integrated Laboratory of Processing Technology for Chinese Meat and Dish Products, Ministry of Agriculture and Rural Affairs, Beijing 100193, China
Collage of Engineering, China Agricultural University, Beijing 100083, China
Collage of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Peer review under responsibility of Beijing Academy of Food Sciences.

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Highlights

• Administration of sturgeon-derived chondroitin sulfate (SCS) alleviates DSS-induced colitis via inflammatory response.

• Administration of SCS can rebuild the gut microenvironment in DSS-induced mice.

• SCS can stimulate the synthesis of bile acids, inhibit the NF-κB signaling pathway, and alleviate DSS-induced colitis.

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Abstract

Chondroitin sulfate (CS) is one of the main bioactive compounds in animal cartilage. In this study, the anti-inflammatory activity of sturgeon-derived chondroitin sulfate (SCS) was evaluated in the dextran sulfate sodium (DSS)-induced BALB/c mice model. Orally administration of SCS significantly alleviated the DSS-induced colitis symptoms, including the reduction of crypt depth, inhibition of the abnormal crypt foci formation, down-regulation of the proinflammatory biomarkers (NO, interleukin (IL)-6, IL-1β and tumor necrosis factor-α) and up-regulation of the anti-inflammatory biomarkers (IL-10 and IL-4). The gut microbiota analysis revealed that SCS alters the intestinal microbiota composition in colitis mice, especially the increase of the relative abundance of Ruminococcaceae and Lachnospiraceae. This alternation further induced primary bile acids convert into secondary bile acids. With SCS administration, the levels of deoxycholic acid (DCA) and litho cholic acid (LCA) were increased by 1.5- and 2.5-fold, respectively. The stimulated secretion of DCA and LCA showed further activation of the NF-κB signaling pathway, thereby suppressing the inflammatory response and attenuating inflammatory bowel disease (IBD) in mice. This study provided a valuable strategy for colitis prevention and treatment with sturgeon cartilage by-products.

Keywords

Sturgeon cartilageChondroitin sulfateAnti-inflammatory activityDextran sulfate sodium-induced colitisGut microbiota

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Food Science and Human Wellness
Volume 14 Issue 4,
April 2025
Article number: 9250227
DOI: 10.26599/FSHW.2024.9250227
Cite this article:
Wu R, Han Z, Wang Z, et al. Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota. Food Science and Human Wellness, 2025, 14(4): 9250227. https://doi.org/10.26599/FSHW.2024.9250227
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