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Research Article | Open Access | Just Accepted

Tri-signal colorimetric/electrochemical detection of egg allergen based on boric acid affinity reagent modified CuO nanoparticles

Chunlan ChenaFengqi ZhouaHongmin ZhuaDing MaaYufan HuangbJing LibLinjiang GuoaLin XiaoaGuobao NingaXiaokang LuaBilian LiaHui Zhaob()Can-Peng Lia()

a School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China

b Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, PR China

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Abstract

A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid (OM) was developed. Herein, copper oxide nanoparticles (CuO NPs) were prepared, which exhibit excellent enzyme-like activity (peroxidase-like and laccase-like) and electrochemical activity. CuO@3-APBA nanoparticles (CuO@3-APBA NPs) were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid (3-APBA) in CuO NPs. 3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions. Then, a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme. For the first signal, CuO@3-APBA can catalyze oxidation of TMB to turn the solution from colorless to blue in the presence of H2O2 (absorbance at 652 nm). For the second signal, CuO@3-APBA can catalyze the oxidation of substrates (2, 4-dichlorophenol and 4-aminoantipyrine) and turn  the solution from colorless to  pink (absorbance at 510 nm). For the third signal, electrochemical oxidation peak of copper ion from Cu+ to Cu2+ of CuO@3-APBA was recorded by differential pulse voltammetry, which was used to determine the OM. The sensing platform exhibited a wide linear range (0.0000316-100 ng/mL) with a low detection limit (0.0105 pg/mL), as well as showed advantages, such as satisfactory reproducibility, good stability, and excellent selectivity. The assay has the potential applications for ultrasensitive detection of allergen in foods.

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Food Science and Human Wellness
Cite this article:
Chen C, Zhou F, Zhu H, et al. Tri-signal colorimetric/electrochemical detection of egg allergen based on boric acid affinity reagent modified CuO nanoparticles. Food Science and Human Wellness, 2024, https://doi.org/10.26599/FSHW.2024.9250330
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