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Research Article | Open Access | Just Accepted

Modulation of oxidative damage and genotoxicity of biosynthesized zinc oxide nanoparticles via supplementation with modified cinnamaldehyde-loaded chitosan nanoparticles in mice

Mosaad A. Abdel Wahhab1( )Aziza A. El Nekeety1Asmaa S. Salman2Zeinab K. Hamza1Engy M. Akl3Soher E. Aly1Wenyi Kang4,5,6

1 Department of Food Toxicology & Contaminants, National Research Centre, Dokki, Cairo, Egypt

2 Genetic and Cytology Department, National Research Centre, Dokki, Cairo, Egypt

3 Department of Fats and Oils, National Research Centre, Dokki, Cairo, Egypt

4 National R & D Center for Edible Fungus Processing Technology, Henan University

5 Joint International Research Laboratory of Food & Medicine Resource Function, Henan, Kaifeng 475004, China

6 Functional Food Engineering Technology Research Center, Henan, Kaifeng 475004, China

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Abstract

Synthesis of zinc oxide nanoparticles (Zn-NPs) via green method is an outstanding alternative to conventional/regular methods; however, the safety or toxicity of the biosynthesized ZnO-NPs in vivo is not fully explored. This study was conducted to evaluate the protective efficiency of cinnamaldehyde-loaded chitosan nanoparticles (Cin@CSNPs) against oxidative damage and genotoxicity of zinc oxide nanoparticles (ZnO-NPs) in mice. ZnO-NPs were biosynthesized using the extract of fresh leaves of Mentha pulegium L.  Cin was extracted from cinnamon essential oil, and was loaded into chitosan nanoparticle (Cin@CSNPs). Both ZnO-NPs, Cin@CSNPs and CSNPs were characterized. The in vitro release of Cin@CSNPs was determined. In the biological study, 6 groups of male Balb/c mice were treated by gavage for 3 weeks as follows, control group, the group received ZnO-NPs (25 mg/kg b.w), the groups received Cin@CSNPs at low dose  (50 mg/kg b.w) or high dose (100 mg/kg b.w), and the groups received ZnO-NPs plus Cin@CSNPs at the two tested doses. Blood and tissue samples were collected for different biochemical, genetical and histological studies. The particle size of ZnO-NPs, CSNPs, and Cin@CSNPs were (20.78 ± 2.6), (170.0 ± 3.7), and (218.23 ± 2.9) nm, and ξ-potential were (32.7 ± 4.6), (8.32 ± 0.27) and (4.8 ± 0.21), respectively. ZnO-NPs disturbed the biochemical and oxidative stress indices, AFP, CEA, TNF-α, chromosomal aberrations in somatic and germ cells, and sperm abnormality along with severe pathological changes in the hepatic, renal, and testicular tissues. Cin@CSNPs improved significantly all the parameters tested and the histological picture in a dose-dependent. Therefore, the biosynthesized ZnO-NPs exhibit oxidative damage and genotoxicity, and Cin@CSNPs have potential protective effects against the risks of ZnO-NPs and may be a promising tool to overcome the challenges of using Cin in food and pharmaceuticals applications.

Food Science and Human Wellness
Cite this article:
Wahhab MAA, Nekeety AAE, Salman AS, et al. Modulation of oxidative damage and genotoxicity of biosynthesized zinc oxide nanoparticles via supplementation with modified cinnamaldehyde-loaded chitosan nanoparticles in mice. Food Science and Human Wellness, 2024, https://doi.org/10.26599/FSHW.2024.9250374

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Received: 17 July 2024
Revised: 20 September 2024
Accepted: 30 September 2024
Available online: 10 October 2024

© Tsinghua University Press 2024

Reprints and Permission requests may be sought directly from editorial office.
Email: nanores@tup.tsinghua.edu.cn

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