Gastrodia elata Blume (G. elata) widely reputed as an important traditional herbal medicine and food in China and other Asian countries, can manage headache, epilepsy, pediatric acute, and chronic convulsions in modern clinical practice. It has been revealed that G. elata contains a variety of chemical substances, which have attracted more and more attention from the food science community. Hence, a comprehensive search of published literature from the beginning to 2024 in Web of Science and CNKI, and the keyword of G. elata was used. This review systematically summarizes analytical methods of bioactive phytochemicals in G. elata. We also depicted known biological harmful constituents in this review. In addition, abundant food products made from G. elata have been developed and sold on market, and different processing methods of G. elata containing foods have significant effects on the bioactive phytochemical composition of G. elata. Hence, we further summarized the impacts of food processing on the development of G. elata related functional food products. In a nutshell, this review summarizes the bioactive phytochemicals, analysis methods, and application of G. elata and its associated food products, and aims to provide a valuable reference for the full utilization of resources of G. elata.
Abdel-Salam, O.M.E., Youness, E.R., Mohammed, N.A., Morsy, S.M.Y., Omara, E.A., and Sleem, A.A. (2014). Citric Acid Effects on Brain and Liver Oxidative Stress in Lipopolysaccharide-Treated Mice. J. Med. Food 17: 588–598.
Abdel-Salam, O.M.E., Youness, E.R., Mohammed, N.A., Yassen, N.N., Khadrawy, Y.A., El-Toukhy, S.E., and Sleem, A.A. (2016). Novel neuroprotective and hepatoprotective effects of citric acid in acute malathion intoxication. Asian Pacific Journal of Tropical Medicine. 9: 1181–1194.
Baghaei, P.A.M., Mogaddam, M.R.A., Farajzadeh, M.A., Mohebbi, A., and Sorouraddin, S.M. (2023). Application of deep eutectic solvent functionalized cobalt ferrite nanoparticles in dispersive micro solid phase extraction of some heavy metals from aqueous samples prior to ICP-OES. J. Food Compos. Anal. 117: 1–11.
Bai, Y., Mo, K., Wang, G.R., Chen, W.L., Zhang, W., Guo, Y.B., and Sun, Z.R. (2021). Intervention of Gastrodin in Type 2 Diabetes Mellitus and Its Mechanism. Frontiers in Pharmacology. 12: 1–14.
Cai, X.Y., Kong, F.G., Wang, R.C., Zhai, S.Y., Guan, X., Zhang, G.R., and Wang, D. (2019). Candida albicans vaginitis in a murine model is reduced by polypeptide-enriched Gastrodia elata extracts. Future Microbiology 14: 839–846.
Chen, C., Li, X.X., Li, J., Xu, Y.M., Jing, X., Wu, S.Q., Liu, X., and Zhang, X.Y. (2018). Purification and characterization of an antimicrobial protein from Gastrodia elata Blume tubers. Trop. J. Pharm. Res. 17: 1717–1723.
Chen, L.H., Wang, Z.H., Mao, Y.Y., Chen, Y.H., and Li, J.Y. (2024). Effect of solid-state co-fermentation on the structural characteristics and bioactivities of polysaccharides in the medicinal residues of Gastrodia Ganoderma. Food Bioscience. 61: 1–11.
Chen, S., Liu, J.Q., Xiao, H., Zhang, J., and Liu, A. (2016). Simultaneous Qualitative Assessment and Quantitative Analysis of Metabolites (Phenolics, Nucleosides and Amino Acids) from the Roots of Fresh Gastrodia elata Using UPLC-ESI-Triple Quadrupole Ion MS and ESI- Linear Ion Trap High-Resolution MS. PLoS One 11: 1–16.
Chen, W.C., Lai, Y.S., Lu, K.H., Lin, S.H., Liao, L.Y., Ho, C.T., and Sheen, L.Y. (2015). Method development and validation for the high-performance liquid chromatography assay of gastrodin in water extracts from different sources of Gastrodia elata Blume. J. Food Drug Anal. 23: 803–810.
Chen, X.B., Li, H.W., Sha, O., Dai, X.C., Wu, Y.F., Xu, Z.X., and Wang, Z.W. (2024). Determination of gastrodin in urine by magnetic dispersion solid phase extraction-high performance liquid chromatography. Chemical Research and Application. 36: 1929–1934.
Cheng, L.J., and Deng, Y. (2021). Characterization by HPLC of hydroxybenzyl alcohol biotransformation to gastrodin in vivo. Nat. Prod. Commun. 16: 1–8.
Dong, J.J., Ji, D., Su, L.L., Zhang, F.Y., Tong, H.J., Mao, C.Q., and Lu, T.L. (2020). A simplified LC-MS/MS approach for simultaneous quantification and pharmacokinetics of five compounds in rats following oral administration of Gastrodia elata extract. J. Anal. Sci. Technol. 11: 1–9.
Du, J.Y., Mo, J.Y., Xie, X., Huang, X.L., Wu, X.L., and Wang, L.S. (2024). Comprehensive quality evaluation of Tianma jiannao granules. China Pharmacy. 35: 2482–2487.
Gan, Q.X., Peng, M.Y., Wei, H.B., Chen, L.L., Chen, X.Y., Li, Z.H., An, G.Q., and Ma, Y.T. (2024). Gastrodia elata polysaccharide alleviates Parkinson’s disease via inhibiting apoptotic and inflammatory signaling pathways and modulating the gut microbiota. Food & Function 15: 2920–2938.
Gong, M.Q., Lai, F.F., Chen, J.Z., Li, X.H., Chen, Y.J., and He, Y. (2024). Traditional uses, phytochemistry, pharmacology, applications, and quality control of Gastrodia elata Blume: A comprehensive review. J. Ethnopharmacol. 319: 1–22.
Guan, H., Ling, X., Xu, J., Zhu, Y.Q., Zhang, J.Y., and Liu, X.Y. (2022). Structural Characterization of Polysaccharide Derived from Gastrodia elata and Its Immunostimulatory Effect on RAW264.7 Cells. Molecules 27: 1–23.
Guan, J., Zhang, X.R., Feng, B., Zhao, D.H., Zhao, T., Chang, S., Wang, L.M., and Zhu, H.Y. (2017). Simultaneous determination of ferulic acid and gastrodin of Tianshu Capsule in rat plasma by ultra-fast liquid chromatography with tandem mass spectrometry and its application to a comparative pharmacokinetic study in normal and migraine rats. J. Sep. Sci. 40: 4120–4127.
Hu, M.H., Yan, H., Fu, Y.Y., Jiang, Y.L., Yao, W.F., Yu, S., Zhang, L., Wu, Q.A., Ding, A.W., and Shan, M.Q. (2019). Optimal Extraction Study of Gastrodin-Type Components from Gastrodia Elata Tubers by Response Surface Design with Integrated Phytochemical and Bioactivity Evaluation. Molecules 24: 1–14.
Huang, T.Y., Danaher, L.A., Brüschweiler, B.J., Kass, G.E.N., and Merten, C. (2019). Naturally occurring bisphenol F in plants used in traditional medicine. Archives of Toxicology. 93: 1485–1490.
Ji, N., Liu, P., Zhang, N., Yang, S.Y., and Zhang, M.S. (2022). Comparison on bioactivities and characteristics of polysaccharides from four varieties of Gastrodia elata Blume. Frontiers in Chemistry. 10: 1–18.
Ji, W.H., Zhang, M.M., Wang, D.J., Wang, X., Liu, J.H., and Huang, L.Q. (2015). Superhydrophilic molecularly imprinted polymers based on a water-soluble functional monomer for the recognition of gastrodin in water media. J. Chromatogr. A 1425: 88–96.
Jiang, N., Yao, C., Zhang, Y., Chen, Y., Chen, F., Luo, Y., Choudhary, M.I., Pan, R., and Liu, X. (2024). Antidepressant effects of Parishin C in chronic social defeat stress-induced depressive mice. J. Ethnopharmacol. 325: 1–12.
Kan, W.L., Ma, B., and Lin, G. (2011). Sulfur Fumigation Processing of Traditional Chinese Medicinal Herbs: Beneficial or Detrimental? Frontiers in Pharmacology. 2: 1–7.
Khan, W.A., Arain, M.B., and Soylak, M. (2020). Nanomaterials-based solid phase extraction and solid phase microextraction for heavy metals food toxicity. Food and Chemical Toxicology. 145: 1–13.
Kong, F., Cai, X.Y., Zhai, S.Y., Wang, R.C., Zheng, X.Y., Ma, Y., Bi, H., and Wang, D. (2019). Possible mechanisms of the antimicrobial effects of polypeptide-enriched Gastrodia elata Blume extracts. Mol. Med. Rep. 20: 4723–4730.
Lee, J.G., Moon, S.O., Kim, S.Y., Yang, E.J., Min, J.S., An, J.H., Choi, E.A., Liu, K.H., Park, E.J., Lee, H.D., and Song, K.S. (2015). Rapid HPLC determination of gastrodin in Gastrodiae Rhizoma. J. Korean Soc. Appl. Biol. Chem. 58: 409–413.
Li, H.X., Ding, M.Y., Lv, K., Wei, Y., and Yu, J.Y. (2001). Identification and determination of the active compounds in Gastrodia elata Blume by HPLC. J. Liq. Chromatogr. Relat. Technol. 24: 579–588.
Li, S.S., Zhang, F.J., Li, L.X., Zhang, H., Duan, X.W., Shi, L., Cui, X.M., and Li, X.Q. (2024). Rapid determination of active ingredient contents in Rhizoma Gastrodiae using near-infrared spectroscopy combined with artificial rabbits optimization-least square support vector regression. Food Science. 45: 207–213.
Li, X.F., Xiang, B., Shen, T., Xiao, C., Dai, R., He, F.Y., and Lin, Q. (2020). Anti-neuroinflammatory effect of 3,4-dihydroxybenzaldehyde in ischemic stroke. International Immunopharmacology. 82: 1–11.
Li, Y.H., Zhang, Y.M., Zhang, Z.J., Hu, Y.P., Cui, X.M., and Xiong, Y. (2019). Quality evaluation of gastrodia elata tubers based on HPLC fingerprint analyses and quantitative analysis of multi-components by single marker. Molecules 24: 1–16.
Li, Z.B., Huang, J., Wang, L., Li, D., Chen, Y.P., Xu, Y.Q., Li, L., Xiao, H., and Luo, Z.S. (2024). Novel insight into the role of sulfur dioxide in fruits and vegetables: Chemical interactions, biological activity, metabolism, applications, and safety. Critical Reviews in Food Science and Nutrition. 64: 8741–8765.
Liu, C.L., Liu, M.C., and Zhu, P.L. (2002). Determination of gastrodin, p-hydroxybenzyl alcohol, vanillyl alcohol, p-hydroxylbenzaldehyde and vanillin in tall Gastrodia tuber by high-performance liquid chromatography. Chromatographia. 55: 317–320.
Liu, J.Q., Chen, S., Cheng, J.T., Zhang, J., Wang, Y.S., and Liu, A. (2017). An optimized and sensitive pharmacokinetic quantitative method of investigating gastrodin, parishin, and parishin B, C and E in beagle dog plasma using LC-MS/MS after intragastric administration of tall Gastrodia capsules. Molecules 22: 1–11.
Liu, Y., Gao, J.L., Peng, M., Meng, H.Y., Ma, H.B., Cai, P.P., Xu, Y., Zhao, Q., and Si, G.M. (2018). A review on central nervous system effects of gastrodin. Frontiers in Pharmacology. 9: 1–18.
Luo, K.X., Wang, Y.H., Chen, W.S., Feng, X.J., Liao, Y.H., Chen, S.C., Liu, Y., Liao, C.D., Chen, M.X., and Ao, L.J. (2022). Treatment combining focused ultrasound with gastrodin alleviates memory deficit and neuropathology in an Alzheimer’s disease-like experimental mouse model. Neural Plast. 2022: 1–13.
Lv, P., Fu, Y.L., Tao, B., Mao, M.D., Zhao, J.Y., and He, Z.Y. (2022). Determination of gastrodin in tianma medicinal liquor by high-performance liquid chromatography. China Port Science and Technology. 4: 83–87.
Pasquini, C. (2018). Near infrared spectroscopy: A mature analytical technique with new perspectives – A review. Analytica Chimica Acta. 1026: 8–36.
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K.M., and Yoga Latha, L. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr. J. Tradit. Complement. Altern. Med. 8: 1–10.
Shan, T.Y., Yin, M.Z., Wu, J.X., Yu, H.W., Liu, M.L., Xu, R., Wang, J.T., Peng, H.S., Zha, L.P., and Gui, S.Y. (2021). Comparative transcriptome analysis of tubers, stems, and flowers of Gastrodia elata Blume reveals potential genes involved in the biosynthesis of phenolics. Fitoterapia. 153: 1–11.
Shao, Q.H., Zhang, X.L., Chen, Y., Zhu, C.G., Shi, J.G., Yuan, Y.H., and Chen, N.H. (2018). Anti-neuroinflammatory effects of 20C from Gastrodia elata via regulating autophagy in LPS-activated BV-2 cells through MAPKs and TLR4/Akt/mTOR signaling pathways. Mol. Immunol. 101: 640–640.
Sharifi, V., Abbasi, A., and Nosrati, A. (2016). Application of hollow fiber liquid phase microextraction and dispersive liquid-liquid microextraction techniques in analytical toxicology. J. Food Drug Anal. 24: 264–276.
Shin, E.-J., Whang, W.K., Kim, S., Bach, J.-H., Kim, J.-M., Nguyen, X.-K.T., Nguyen, T.-T.L., Jung, B.D., Yamada, K., Nabeshima, T., and Kim, H.-C. (2010). Parishin C attenuates phencyclidine-induced schizophrenia-like psychosis in mice: Involvements of 5-HT1A Receptor. J. Pharmacol. Sci. 113: 404–408.
Si, K.Y., Yan, C.R., Huang, Y.P., Kong, D.T., Yang, J.J., Wang, L.H., Liu, X.Y., and Hou, Y. (2023). Study by the rapid determination of the content of four components in gastrodia based on near-infrared spectroscopy. Yunnan Chem. Technol. 50: 65–68.
Song, Y.L., An, X.L., and Kong, P. (2024). HPLC determination of gastrodin and gastrodigenin in Tianma Xifeng capsules. China Journal of Pharmaceutical Economics. 19: 108–111.
Su, Z.H., Yang, Y.G., Chen, S.Z., Tang, Z.S., and Xu, H.B. (2023). The processing methods, phytochemistry and pharmacology of Gastrodia elata Bl.: A comprehensive review. J. Ethnopharmacol. 314: 1–32.
Tan, A., Hussain, S., Musuku, A., and Massé, R. (2009). Internal standard response variations during incurred sample analysis by LC–MS/MS: Case by case trouble-shooting. J. Chromatogr. B 877: 3201–3209.
Tan, Y.L., Fu, Y.C., Huang, R., Liu, L.Y., Li, X., Luo, Y.D., and Gao, M.X. (2024). Analytical study on quality of Wufeng Gastrodia elata from Hubei province and changes of characteristic flavor substances during fermentation. Food and Fermentation Industries 1–10.
Tang, C.L., Wu, B.C., Wu, J.Y., Zhang, Z., and Yu, B.C. (2018). Novel Strategies Using total gastrodin and gastrodigenin, or total gastrodigenin for quality control of Gastrodia elata. Molecules 23: 1–11.
Teo, C.C., Tan, S.N., Yong, J.W.H., Hew, C.S., and Ong, E.S. (2008). Evaluation of the extraction efficiency of thermally labile bioactive compounds in Gastrodia elata Blume by pressurized hot water extraction and microwave-assisted extraction. J. Chromatogr. A 1182: 34–40.
Tu, Y.J., Ju, S.Q., and Wang, P.Y. (2016). Flame atomic absorption spectrometric determination of copper, lead, and cadmium in Gastrodiae rhizoma samples after preconcentration using magnetic solid-phase extraction. Spectrosc. Lett. 49: 249–256.
Wang, C.H., Zeng, Y.J., Hou, Y.B., Zeng, C.H., Wang, M., and Wang, Y.H. (2023). Effects of Armillaria species on growth and quantity of active medicinal components of G. elata BL.f. elata tubers along an altitude gradient: Evidence from empirical experiments. Phytochem. Lett. 54: 101–106.
Wang, J.-W., Liu, Y.-M., Zhao, X.-F., and Zhang, H. (2017). Gastrodin protects retinal ganglion cells through inhibiting microglial-mediated neuroinflammation in an acute ocular hypertension model. Int J Ophthalmol. 10: 1483–1489.
Wang, Q., Li, Z., Wang, D., Yang, S., and Feng, Y. (2020). Myocardial protection properties of parishins from the roots of Gastrodia elata Bl. Biomedicine & Pharmacotherapy. 121: 1–9.
Wang, T., Chen, H., Xia, S., Chen, X., Sun, H., and Xu, Z. (2021). Ameliorative effect of parishin C against cerebral ischemia-induced brain tissue injury by reducing oxidative stress and inflammatory responses in rat model. Neuropsychiatr. Dis. Treat. 17: 1811–1823.
Wang, X., Li, B., and Tang, K. (2019). Determination of gastrodin in Tianlitong tablets by HPLC. Food and Drug. 21: 199–201.
Wang, Y., Zhao, L., and Li, A.Y. (2021). Gastrodin - A potential drug used for the treatment of Tourette Syndrome. J. Pharmacol. Sci. 145: 289–295.
Wang, Z.J., Zuo, C.Z., Chen, M., Song, J., Tu, K., Lan, W.J., Li, C.Y., and Pan, L.Q. (2023). A novel variable selection method based on ordered predictors selection and successive projections algorithm for predicting gastrodin content in fresh Gastrodia elata using fourier transform near-infrared spectroscopy and chemometrics. Foods. 12: 1–14.
Wu, Y.N., Zhang, H.W., Zhu, J.G., Zhang, Z.L., Ma, S.B., Zhao, Y.Q., Wang, Y.M., Yuan, J., Guo, X., Li, Y.J., and Zhang, S. (2024). The effect of fermentation on the chemical constituents of gastrodia tuber hallimasch powder (GTHP) estimated by UHPLC-Q-Orbitrap HRMS and HPLC. Molecules 29: 1–24.
Wu, Z., Gao, R.P., Li, H., Liao, X., Tang, X., Wang, X.G., and Su, Z.M. (2022). How steaming and drying processes affect the active compounds and antioxidant types of Gastrodia elata Bl. f. glauca S. chow. Food Research International. 157: 1–16.
Xie, Y.K., Li, X.Y., Chen, C., Zhang, W.P., Yu, X.L., Xiao, H.W., and Lu, F.Y. (2023). Effects of steam and water blanching on drying characteristics, water distribution, microstructure, and bioactive components of Gastrodia Elata. Plants 12: 1–17.
Xie, Y.K., Li, X.Y., Zhang, Y., Zheng, Z.A., Huang, L.Q., Liu, D.H., Xiao, H.W., and Liu, Y.H. (2021). Effects of high-humidity hot air impingement steaming on Gastrodia elata: steaming degree, weight loss, texture, drying kinetics, microstructure and active components. Food and Bioproducts Processing. 127: 255–265.
Xu, Q., Liu, Y., Wang, X.C., Gu, H.Y., and Chen, Z.L. (1998). Purification and characterization of a novel anti-fungal protein from Gastrodia elata. Plant Physiol. Biochem. 36: 899–905.
Yan, J.Y., Yang, Z.S., Zhao, N.H., Li, Z.W., and Cao, X. (2019). Gastrodin protects dopaminergic neurons via insulin-like pathway in a Parkinson’s disease model. BMC Neuroscience. 20: 1–11.
Yang, F., Long, Z.X., Lu, W.Y., Liu, Y., and Zhu, J.J. (2023). Determination of gastrodin and ginsenosides in Gastrodia and Ginseng Buccal tablets. Xiandaishipin 29: 218–222.
Yang, L., Qin, S.H., and Zi, C.T. (2024). Research progress of Gastrodia elata Blume polysaccharides: a review of chemical structures and biological activities. Frontiers in Chemistry. 12: 1–9.
Yang, L., Wang, X., Xin, X., and Wang, M.Z. (2011). The determination method research of gastrodin in liver and kidney of rats. Lishizhen Med. Mater. Med. Res. 22: 295–298.
Yu, B.C., Li, Z., Wu, J.Y., Ying, J.M., Tang, Y.Q., Wu, B.C., Tang, C.L., and Xu, J.Y. (2020). Quality control of Gastrodia elata by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and principal component analysis (PCA) and hierarchical cluster analysis (HCA). Analytical Letters. 53: 746–759.
Zeng, X., Li, Y.Y., Ling, H., Chen, J., and Guo, S.X. (2018). Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis. Botanical Studies. 59: 1–8.
Zhang, M.L., Zhang, Y.W., Peng, J.Y., Huang, Y.Y., Gong, Z.P., Lu, H.X., Han, L., and Wang, D.D. (2024). Gastrodin against oxidative stress-inflammation crosstalk via inhibiting mtDNA/TLR9 and JAK2/STAT3 signaling to ameliorate ischemic stroke injury. International Immunopharmacology. 141: 1–14.
Zhang, W., Sheng, Y.X., and Zhang, J.L. (2008). Determination and pharmacokinetics of gastrodin and p-hydroxybenzylalcohol after oral administration of Gastrodia elata Bl. extract in rats by high-performance liquid chromatography-electrospray ionization mass spectrometric method. Phytomedicine 15: 844–850.
Zhao, C.C., Hu, Q., Zhang, X., Yan, H., Li, J., Chen, F., and Zuo, Z.Y. (2023). Molecularly imprinted polymers with ionic liquid as the functional monomer for selective solid-phase extraction of gastrodin. J. Dispersion Sci. Technol.
Zhao, M., Wang, Y., Yang, J., Xie, C.Z., Li, L.L., Zhang, C.J., and Yang, X.S. (2022). Optimization of fermentation process and quality evaluation of Gastrodia elata Jiaosu. China Brewing. 41: 177–182.
Zhao, M.H., Zhou, Y.T., Sheng, R.L., Zhang, H.J., Xiang, J.B., Wang, J., Li, P., Ma, T.Y., Liu, P.W., Chen, Q., Wen, W., and Xu, S.J. (2024). Gastrodin relieves Parkinson’s disease-related motor deficits by facilitating the MEK-dependent VMAT2 to maintain dopamine homeostasis. Phytomedicine 132: 1–11.
Zheng, D., Zhang, T., Huang, J., Wang, M., Cao, Z., Huang, Y., Yang, Z., Deng, Y., and Fang, Y. (2022). Indole-incorporated-benzoeindolium as a novel mitochondrial and ratiometric fluorescent probe for real-time tracking of SO2 derivatives in vivo and herb samples. Dyes and Pigments. 198: 1–10.
Zhu, H.D., Liu, C., Hou, J.J., Long, H.L., Wang, B., Guo, D.A., Lei, M., and Wu, W.Y. (2019). Gastrodia elata Blume Polysaccharides: A Review of Their Acquisition, Analysis, Modification, and Pharmacological Activities. Molecules 24: 1–18.