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Research Article | Open Access

Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats

Meng-Bei Zhang1, Chao-Chao Song2, Guang-Zu Li1, Lan-Fen Chen1, Rui Ma1, Xiao-He Yu3, Ping Gong1, Xiao-Li Wang1( )
Department of Medical Imaging, Weifang Medical University, Weifang 261053, Shandong, China
Department of Digestive System, People’s Hospital of Fangzi District, Weifang 261200, Shandong, China
Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
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Abstract

Objective:

This study aims to observe the effects of transplantation of umbilical cord blood mononuclear cells (UCBMCs) on the expression of interleukin (IL)-1β and explore the mechanism via the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway in hypoxic-ischemic neonatal rats.

Methods:

Seven-day-old Sprague-Dawley neonatal rats were randomly divided into Sham, hypoxic-ischemic brain damage (HIBD), and UCBMC groups. The HIBD model was prepared by Rice-Vannucci method, and UCBMC were transplanted 24 h after HIBD in the UCBMC group. At 7 days after transplantation, changes in neurons and the TLR4 protein were examined by neuronal nuclei (NeuN)/TLR4 immunofluorescence staining. The expression of pNF-κB and IL-1β proteins was detected by immunohistochemical staining and enzyme linked immunosorbent assay (ELISA).

Results:

The percentage of NeuN+DAPI+ cells in the injured cortex in the UCBMC group was significantly higher than that in the HIBD group and lower than that in the Sham group (P < 0.05). The number of NeuN+TLR4+DAPI+cells in the UCBMC group was significantly lower than that in the HIBD group (P < 0.05) but higher than that in the Sham group (P < 0.05). More pNF-κB+ cells were observed in the HIBD group than in Sham and UCBMC groups (P < 0.05), and more pNF-κB+ cells were observed in the UCBMC group than in the Sham group (P < 0.05). ELISA results showed that the IL-1β expression in the injured cerebral cortex in the UCMBC group was significantly lower than that in the HIBD group but remained higher than that in the Sham group (P < 0.05).

Conclusions:

UCBMC transplantation could inhibit the IL-1β protein expression in the injured cortex, thereby alleviating HIBD in neonatal rats. The underlying mechanism might be associated with the down- regulation of TLR4 and pNF-κB proteins.

Keywords

umbilical cord blood mononuclear cell (UCBMC)hypoxic ischemic brain damage (HIBD)toll-like receptor 4 (TLR4)nuclear factor-kappa B (NF-κB)TLR4/NF-κB pathwayneonatal rats

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Journal of Neurorestoratology
Volume 8 Issue 2,
June 2020
Pages 122-130
DOI: 10.26599/JNR.2020.9040015
Cite this article:
Zhang M-B, Song C-C, Li G-Z, et al. Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats. Journal of Neurorestoratology, 2020, 8(2): 122-130. https://doi.org/10.26599/JNR.2020.9040015

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Received: 21 May 2020
Revised: 11 June 2020
Accepted: 15 June 2020
Published: 04 August 2020
© The authors 2020

This article is published with open access at http://jnr.tsinghuajournals.com
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