Graphical Abstract
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Kidney disease, as a global health problem, can progress to kidney failure. Kidney transplantation is a treatment option for end-stage disease and an alternative to dialysis. Complement-dependent cytotoxicity tests and flow cytometry are methods used to test for compatibility between autoantibodies and donor recipient. Antibodies against donor human leukocyte antigens (HLAs), linked to hyperacute or severely acute rejection, frequently result in graft loss. Currently, fluorescent bead assay (Luminex®) technology is used in Thailand for HLA–antibody typing tests. However, each test requires specific location and time, expensive equipment, and high costs. The goal of such research is that, HLA–antibody typing tests can be performed wherever it is the most convenient for recipients and hospitals. A further advantage is that the surgeon can perform a final test for confirmation before the surgery. Numerous technologies are currently available for this purpose. The focus of this study was on the Fe3O4 magnetic nanoparticles (MNPs) immobilized with HLAs frequently found in the Thai population. These were examined using nuclear magnetic resonance (NMR) to measure HLA antibody typing. MNPs have recently attracted considerable attention because of their strong magnetization and large surface areas. Immobilization reactions with antigens and the straightforward magnetic separation of MNPs are low-cost techniques. Hence, the commercialization of Fe3O4 MNPs is favorable. In this study, MNPs were functionalized with 3-aminopropyl triethoxysilane (APTES) in toluene and ethanol, and the nanoparticles were streptavidin activated after APTES treatment. A specific biotinylated HLA for the Thai population was used and immobilized on streptavidin. The MNPs were effectively immobilized. The magnetic properties can be modified by adding other ions to Fe3O4. We discovered that the shape and size variations of Fe3O4 MNPs may have an impact on the amount of functionalized and immobilized surface area.
N. Larpparisuth, W. Cheungpasitporn, A. Lumpaopong. Global perspective on kidney transplantation: Thailand. Kidney360, 2021, 2(7): 1163−1165. https://doi.org/10.34067/KID.0002102021
P. Kupatawintu, S. Pheancharoen, A. Srisuddee, et al. HLA-A, -B, -DR haplotype frequencies in the Thai stem cell donor registry. Tissue Antigens, 2010, 75(6): 730−736. https://doi.org/10.1111/j.1399-0039.2010.01450.x
B.H. Chung, Y.Y. Joo, J. Lee, et al. Impact of ABO incompatibility on the development of acute antibody-mediated rejection in kidney transplant recipients presensitized to HLA. PLoS One, 2015, 10(4): e0123638. https://doi.org/10.1371/journal.pone.0123638
B. Clark, D.J. Unsworth. HLA and kidney transplantation. Journal of Clinical Pathology, 2010, 63(1): 21−25. https://doi.org/10.1136/jcp.2009.072785
E. Lougee, S. Morjaria, O. Shaw, et al. A new approach to HLA typing designed for solid organ transplantation: Epityping and its application to the HLA-a locus. International Journal of Immunogenetics, 2013, 40(6): 445−452. https://doi.org/10.1111/iji.12053
B.D. Tait. Detection of HLA antibodies in organ transplant recipients-triumphs and challenges of the solid phase bead assay. Frontiers in Immunology, 2016, 7: 570. https://doi.org/10.3389/fimmu.2016.00570
G. Wang, C. Wang, R. Yang, et al. A sensitive and stable surface plasmon resonance sensor based on monolayer protected silver film. Sensors, 2017, 17(12): E2777. https://doi.org/10.3390/s17122777
J.T. Liu, P.S. Lin, Y.M. Hsin, et al. Surface plasmon resonance biosensor for microalbumin detection. Journal of the Taiwan Institute of Chemical Engineers, 2011, 42(5): 696−700. https://doi.org/10.1016/j.jtice.2011.01.005
H. Ahn, H. Song, J.R. Choi, et al. A localized surface plasmon resonance sensor using double-metal-complex nanostructures and a review of recent approaches. Sensors, 2017, 18(1): E98. https://doi.org/10.3390/s18010098
N. Kostevšek. A review on the optimal design of magnetic nanoparticle-based T2 MRI contrast agents. Magnetochemistry, 2020, 6(1): 11. https://doi.org/10.3390/magnetochemistry6010011
O.V. Shynkarenko, S.A. Kravchenko. Surface plasmon resonance sensors: Methods of surface functionalization and sensitivity enhancement. Theoretical and Experimental Chemistry, 2015(5): 273. https://doi.org/10.1007/s11237-015-9427-5
L.A. Lyon, M.D. Musick, M.J. Natan. Colloidal Au-enhanced surface plasmon resonance immunosensing. Analytical Chemistry, 1998, 70(24): 5177−5183. https://doi.org/10.1021/ac9809940
Y. Sahoo, A. Goodarzi, M.T. Swihart, et al. Aqueous ferrofluid of magnetite nanoparticles: fluorescence labeling and magnetophoretic control. The Journal of Physical Chemistry B, 2005, 109(9): 3879−3885. https://doi.org/10.1021/jp045402y
Y. Mizukoshi, S. Seino, K. Okitsu, et al. Sonochemical preparation of composite nanoparticles of Au/gamma-Fe2O3 and magnetic separation of glutathione. Ultrasonics Sonochemistry, 2005, 12(3): 191−195. https://doi.org/10.1016/j.ultsonch.2003.12.003
V.M. Costa, M.C.M. de Souza, P.B.A. Fechine, et al. Nanobiocatalytic systems based on lipase-Fe3O4 and conventional systems for isoniazid synthesis: A comparative study. Brazilian Journal of Chemical Engineering, 2006, 33: 661−673. https://doi.org/10.1590/0104-6632.20160333s20150137
J.K. Xu, J.J. Sun, Y.J. Wang, et al. Application of iron magnetic nanoparticles in protein immobilization. Molecules, 2014, 19(8): 11465−11486. https://doi.org/10.3390/molecules190811465
J.C. Gorga, A. Dong, M.C. Manning, et al. Comparison of the secondary structures of human class I and class II majors histocompatibility complex antigens by Fourier transform infrared and circular dichroism spectroscopy. Proceedings of the National Academy of Sciences, 1989, 86(7): 2321−2325. https://doi.org/10.1073/pnas.86.7.2321
N. Gunawansa, R. Rathore, A. Sharma, et al. Crossmatch strategies in renal transplantation: A practice guide for the practicing clinician. Journal of Transplant Surgery, 2017, 1(1): 8−15. https://doi.org/10.36959/338/325
S. Ferrari-Lacraz, J.M. Tiercy, J. Villard. Detection of anti-HLA antibodies by solid-phase assay in kidney transplantation: Friend or foe. Tissue Antigens, 2012, 79(5): 315−325. https://doi.org/10.1111/j.1399-0039.2012.01853.x
H.Y. Song, J. Hobley, X. Su, et al. End-on covalent antibody immobilization on dual polarization interferometry sensor chip for enhanced immuno-sensing. Plasmonics, 2014, 9(4): 851−858. https://doi.org/10.1007/s11468-014-9680-9
N.K. Mehra, A.K. Baranwal. Clinical and immunological relevance of antibodies in solid organ transplantation. International Journal of Immunogenetics, 2016, 43(6): 351−368. https://doi.org/10.1111/iji.12294
R.J. Duquesnoy, M. Marrari. Correlations between terasaki’s HLA class I epitopes and HLAMatchmaker-defined eplets on HLA-A, -B and-C antigens. Tissue Antigens, 2009, 74(2): 117−133. https://doi.org/10.1111/j.1399-0039.2009.01271.x
J.M. Greene, R.W. Wiseman, S.M. Lank, et al. Differential MHC class I expression in distinct leukocyte subsets. BMC Immunology, 2011, 12: 39. https://doi.org/10.1186/1471-2172-12-39
P. Molek, T. Bratkovič. Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries. Acta Chimica Slovenica, 2016, 63(4): 914−919. https://doi.org/10.17344/acsi.2016.2458
A. Man-Kupisinska, M. Michalski, A. Maciejewska, et al. A new ligand-based method for purifying active human plasma-derived ficolin-3 complexes supports the phenomenon of crosstalk between pattern-recognition molecules and immunoglobulins. PLoS One, 2016, 11(5): e0156691. https://doi.org/10.1371/journal.pone.0156691
N. Sun, Y. Liu, H. Lee, et al. CMOS RF biosensor utilizing nuclear magnetic resonance. IEEE Journal of Solid-State Circuits, 2009, 44(5): 1629−1643. https://doi.org/10.1109/jssc.2009.2017007
K.M. Lei, P.I. Mak, M.K. Law, et al. A palm-size μNMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis. The Analyst, 2015, 140(15): 5129−5137. https://doi.org/10.1039/c5an00500k
Y.L. Luo, E.C. Alocilja. Portable nuclear magnetic resonance biosensor and assay for a highly sensitive and rapid detection of foodborne bacteria in complex matrices. Journal of Biological Engineering, 2017, 11: 14. https://doi.org/10.1186/s13036-017-0053-8
S. Sabban, H.T. Ye, B. Helm. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its hight-affinity FcεRI receptor. Jounrnal of Visualized Experiments, 2014, 93: e52222. https://doi.org/10.3791/52222
A.K. Gupta, R.R. Naregalkar, V.D. Vaidya, et al. Recent advances on surface engineering of magnetic iron oxide nanoparticles and their biomedical applications. Nanomedicine, 2007, 2(1): 23−39. https://doi.org/10.2217/17435889.2.1.23
K.S. Park, H. Kim, S. Kim, et al. Nanomagnetic system for rapid diagnosis of acute infection. ACS Nano, 2017, 11(11): 11425−11432. https://doi.org/10.1021/acsnano.7b06074
H.Y. Lin, C.H. Huang, J. Park, et al. Integrated magneto-chemical sensor for on-site food allergen detection. ACS Nano, 2017, 11(10): 10062−10069. https://doi.org/10.1021/acsnano.7b04318
A.C. Barreto, F.J.N. Maia, V.R. Santiago, et al. Novel ferrofluids coated a renewable material obtained from cashew nut shell liquid. Microfluidics and Nanofluidics, 2011, 12(5): 677−686. https://doi.org/10.1007/s10404-011-0910-6
Can, K., Ozmen, M., Ersoz, M. Immobilization of albumin on aminosilane modified superparamagnetic magnetite nanoparticles and its characterization. Colloids and Surfaces B,Biointerfaces, 2009, 71(1): 154−159. https://doi.org/10.1016/j.colsurfb.2009.01.021
J.S. Choi, S. Kim, D. Yoo, et al. Distance-dependent magnetic resonance tuning as a versatile MRI sensing platform for biological targets. Nature Materials, 2017, 16(5): 537−542. https://doi.org/10.1038/NMAT4846
J.T. Jang, H. Nah, J.H. Lee, et al. Critical enhancements of MRI contrast and hyperthermic effects by dopant-controlled magnetic nanoparticles. Angewandte Chemie, 2009, 48(7): 1234−1238. https://doi.org/10.1002/ange.200805149
A. Cavalli, X. Salvatella, C.M. Dobson, et al. Protein structure determination from NMR chemical shifts. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(23): 9615−9620. https://doi.org/10.1073/pnas.0610313104
R. Li, F. Feng, Z.Z. Chen, et al. Sensitive detection of carcinoembryonic antigen using surface plasmon resonance biosensor with gold nanoparticles signal amplification. Talanta, 2015, 140: 143−149. https://doi.org/10.1016/j.talanta.2015.03.041
W.A. Talavera-Pech, A. Esparza-Ruiz, P. Quintana-Owen, et al. Effects of different amounts of APTES on physicochemical and structural properties of amino-functionalized MCM-41- MSNs. Journal of Sol-Gel Science and Technology, 2016, 80(3): 697−708. https://doi.org/10.1007/s10971-016-4163-4
S. Sajjadifar, Z. Gheisarzadeh. Isatin-SO3H coated on amino propyl modified magnetic nanoparticles (Fe3O4@APTES@isatin-SO3H) as a recyclable magnetic nanoparticle for the simple and rapid synthesis of pyrano[2,3-d] pyrimidines derivatives. Applied Organometallic Chemistry, 2018, 33(1): e4602. https://doi.org/10.1002/aoc.4602
B.T. Thanh, N. Van Sau, H. Ju, et al. Immobilization of protein A on monodisperse magnetic nanoparticles for biomedical applications. Journal of Nanomaterials, 2019, 2019: 1−9. https://doi.org/10.1155/2019/2182471
B. Thangaraj, Z. Jia, L. Dai, et al. Effect of silica coating on Fe3O4 magnetic nanoparticles for lipase immobilization and their application for biodiesel production. Arabian Journal of Chemistry, 2016, 12(8): 4694−4706. https://doi.org/10.1016/j.arabjc.2016.09.004
S.F. Chew, B.R. Wood, C. Kanaan, et al. Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody. Tissue Antigens, 2007, 69(Suppl 1): 252−258. https://doi.org/10.1111/j.1399-0039.2006.00775.x
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