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Research Article | Open Access

Exploring the Anti-bacterial Potential of Orchid-derived Silver Nanoparticles

Kandasamy Saravanan1Lakshmi Prabha1( )Kumarappan Chidambaram2( )Anandhalakshmi Subramanian3Arunachalam Kalirajan4Nandagopalan Veeraiyan5Kaliamoorthy Seventhilingam6Selvaraj Karthik7Kaja Abdhul7Chellaiah Ramalakshmi8
Department of Botany, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India
Department of Pharmacology, College of Pharmacy, King Khalid University, Abha 61421, Saudi Arabia
Department of Microbiology and Clinical Parasitology, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia
Department of Chemistry and Biology, School of Natural and Applied Sciences, Mulungushi University, Kabwe 80415, Zambia
Department of Botany, National College (Autonomous), Tiruchirappalli 620001, Tamil Nadu, India
National Orchidarium and Experimental Garden, Botanical Survey of India, Southern Regional Centre, Yercaud 636602, Tamil Nadu, India
Post-Graduate & Research Department of Biotechnology, Nandha Arts and Science College, Erode 638052, Tamil Nadu, India
Department of Zoology, Sri Parasakthi College for Women, Courtallum 627802, Tamil Nadu, India
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Abstract

In this study, silver nanoparticles (AgNPs) were synthesized in an environmentally friendly manner using plant extracts from Luisia tristis. The formation of the nanoparticles was confirmed by a reddish-brown colour change and further characterized using ultraviolet–visible (UV–Vis), Fourier transform infrared spectrometer (FTIR), scanning electron microscope (SEM), and transmission electron microscope (TEM) techniques. The average size of the particles was found to be 16–48 nm. The antimicrobial activity of the AgNPs was evaluated against harmful bacteria and compared to the commonly used antibiotic ciprofloxacin. The AgNPs were found to be highly effective, with a 24 mm zone of inhibition against Escherichia coli, and more effective than ciprofloxacin. Additionally, a minimum inhibitory concentration assay was performed with a concentration of 100 mg/mL of AgNPs, which were found to effectively inhibit the growth of selected pathogens. Overall, the study demonstrates the potential for using plant-derived AgNPs as a natural and eco-friendly alternative for antimicrobial and antioxidant applications. This method is a fast, cost-effective way to generate silver nanoparticles at room temperature and may be useful in creating environmentally friendly antibacterial solutions for biomedical applications.

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Nano Biomedicine and Engineering
Pages 416-424
Cite this article:
Saravanan K, Prabha L, Chidambaram K, et al. Exploring the Anti-bacterial Potential of Orchid-derived Silver Nanoparticles. Nano Biomedicine and Engineering, 2023, 15(4): 416-424. https://doi.org/10.26599/NBE.2023.9290038

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Received: 01 June 2023
Revised: 02 September 2023
Accepted: 13 September 2023
Published: 20 November 2023
© The Author(s) 2023.

This is an open-access article distributed under  the  terms  of  the  Creative  Commons  Attribution  4.0 International  License (CC BY) (http://creativecommons.org/licenses/by/4.0/), which  permits  unrestricted  use,  distribution,  and reproduction in any medium, provided the original author and source are credited.

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