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Research Article | Open Access

Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala

Department of Biological Science, School of Life Science, Nanchang University, Nanchang 330031, China
Laboratory of Fear and Anxiety Disorders, Institutes of Life Science, Nanchang University, Nanchang 330031, China
Queen Mary School, Nanchang University, Nanchang 330001, China
School of Basic Medical Sciences, Nanchang University, Nanchang 330031, China
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Abstract

Amygdala is widely known as a critical neural hub for modulating negative emotions such as fear and anxiety. Recent studies have shown that the basolateral amygdala (BLA) has reciprocal innervation, which plays an important role in regulating social interaction and associative memory. However, the connectivity pattern and functional role of the contralateral projecting BLA neurons in stress-related anxiety are not fully understood. Here, using the virus tracing strategy, we first characterized the connectivity pattern of the contralateral projecting neurons in BLA. Then, we examined the effect of stress on the functional changes of those specific neuronal populations using a mouse model of inescapable footshock stress. The results showed that the fibers of the contralateral projecting BLA neurons labeled by enhanced green fluorescent protein (EGFP) have no collateral projections to the ipsilateral or contralateral hippocampus. Interestingly, they have some collateral projections to the ipsilateral medial prefrontal cortex. Behavioral results showed that footshock stress increases the anxiety-like behavior in mice; however, the excitatory synaptic transmission and intrinsic excitability of the contralateral projecting BLA neurons measured by whole-cell patch clamp recording did not change after stress exposure. In conclusion, our results indicate that contralateral projecting BLA neurons may not contribute to footshock stress-related anxiety-like behavior.

Keywords

amygdalacontralateral connectivitystressanxietycircuit

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Stress and Brain
Volume 2 Issue 3,
September 2022
Pages 87-99
DOI: 10.26599/SAB.2022.9060017
Cite this article:
Zou J-X, Shang G-Q, Liu W-Z, et al. Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala. Stress and Brain, 2022, 2(3): 87-99. https://doi.org/10.26599/SAB.2022.9060017

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Received: 15 July 2022
Revised: 11 August 2022
Accepted: 24 August 2022
Published: 09 September 2022
© The Author(s) 2022

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attributtion-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission.
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