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Open Access

Prediction of Cancer-Associated piRNA–mRNA and piRNA–lncRNA Interactions by Integrated Analysis of Expression and Sequence Data

Yajun Liu, Junying Zhang( ), Aimin Li, Zhaowen Liu, Zhongzhen He, Xiguo Yuan, Shouheng Tuo
Xi’an University of Technology, Xi’an 710048, China.
School of Computer Science and Technology, Xidian University, Xi’an 710071, China.
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Abstract

piwi-interacting RNAs (piRNAs) are valuable biomarkers, but functional studies are still very limited. Recent research shows that piRNA-mediated cleavage acts on Transposable Elements (TEs), messenger RNAs (mRNAs), and long non-coding RNAs (lncRNAs). This study aimed to predict cancer-associated piRNA-mRNA and piRNA-lncRNA interactions as well as piRNA regulatory functions. Four cancer types (BRCA, HNSC, KIRC, and LUAD) were investigated. Interactions were identified by integrated analysis of the expression and sequence data. For the expression analysis, only piRNA–mRNA and piRNA–lncRNA pairs with expression profiles that were significantly inversely correlated were retained to reduce false-positive rates during the prediction. For the sequence analysis, miRanda was used for the target prediction. We identified 198 piRNA–mRNA and 10 piRNA–lncRNA pairs. Unlike mRNA and lncRNA expressions, the piRNA expression was relatively consistent across the cancer types. Furthermore, the identified piRNAs were consistent with previously published cancer biomarkers, such as piRNA-36741, piR-21032, and piRNA-57125. More importantly, predicted piRNA functions were determined by constructing an interaction network, and piRNA targets were placed in gene ontology categories related to the cancer hallmarks “activating invasion and metastasis” and “sustained angiogenesis”.

Keywords

co-expressionpiRNA-mRNA interactionpiRNA-lncRNA interactionintegrated analysistarget prediction

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Tsinghua Science and Technology
Volume 23 Issue 2,
April 2018
Pages 115-125
DOI: 10.26599/TST.2018.9010056
Cite this article:
Liu Y, Zhang J, Li A, et al. Prediction of Cancer-Associated piRNA–mRNA and piRNA–lncRNA Interactions by Integrated Analysis of Expression and Sequence Data. Tsinghua Science and Technology, 2018, 23(2): 115-125. https://doi.org/10.26599/TST.2018.9010056

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Received: 07 November 2016
Revised: 07 March 2017
Accepted: 10 March 2017
Published: 02 April 2018
© The author(s) 2018
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