Optimization of ultrasonic-assisted extraction of bioactive compounds from Bupleuri Radix by response surface methodology and HPLC analysis
), Hui Zhaoa()Abstract
A simple and efficient ultrasonic-assisted extraction of bioactive compounds from raw Bupleuri Radix and vinegar-baked Bupleuri Radix was developed and optimized by using quantitative analysis of multi-components by a single-marker combined with response surface methodology approaches. The quantitative analysis of multi-components by a single-marker analytical method based on high-performance liquid chromatography coupled with a photodiode array detector was established and applied to determine the contents of six bioactive compounds in raw Bupleuri Radix and vinegar-baked Bupleuri Radix. In order to maximize extraction of six bioactive compounds in raw Bupleuri Radix and vinegar-baked Bupleuri Radix, the ultrasonic-assisted extraction conditions were obtained by the response surface methodology coupled with Box–Behnken design. The conditions of ultrasonic-assisted extraction were found to be effective for the extraction of bioactive compounds from raw Bupleuri Radix and vinegar-baked Bupleuri Radix. The test results showed that the difference in saikosaponins contents in these samples was obvious; a quantitative analysis of saikosaponins will contribute significantly to improving the quality control of raw Bupleuri Radix and vinegar-baked Bupleuri Radix. In conclusion, the designed protocol is suitable for the quality control of raw Bupleuri Radix and vinegar-baked Bupleuri Radix.
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References
Bao, Y., Li, C., Shen, H., and Nan, F. (2004). Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the chinese multiherb remedy xiaochaihu-tang using liquid chromatographic tandem mass spectrometry. Anal. Chem. 76(14): 4208–4216.
Chiang, L.C., Ng, L.T., Liu, L.T., Shieh, D.E., and Lin, C.C. (2003). Cytotoxicity and anti-hepatitis B virus activities of saikosaponins from Bupleurum species. Planta Med. 69(08): 705–709.
Hu, Z., Cai, M., and Liang, H.H. (2008). Desirability function approach for the optimization of microwave-assisted extraction of saikosaponins from Radix Bupleuri. Sep. Purif. Technol. 61(3): 266–275.
Huang, W., Xue, A., Niu, H., Jia, Z., and Wang, J. (2009). Optimised ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro. Food Chem. 114(3): 1147–1154.
Kato, M., Pu, M.Y., Isobe, K.I., Iwamoto, T., Nagase, F., Lwin, T., and Nakashima, I. (1994). Characterization of the immunoregulatory action of saikosaponin-d. Cell. Immunol. 159(1): 15–25.
Li, F., Cao, Q.E., and Ding, Z. (2004). Separation and determination of five anthraquinones in rheum and rheum-containing preparations by capillary zone electrophoresis. Chromatographia 59(11): 753–757.
Li, X.Q., Gao, Q.T., Chen, X.H., and Bi, K.S. (2005). High performance liquid chromatographic assay of saikosaponins from Radix Bupleuri in China. Biol. Pharm. Bull. 28(9): 1736–1742.
Matsuda, H., Murakami, T., Ninomiya, K., Inadzuki, M., and Yoshikawa, M. (1997). New hepatoprotective saponins, bupleurosides Ⅲ, Ⅵ, Ⅸ, and ⅫⅠ, from Chinese Bupleuri Radix: Structure-requirements for the cytoprotective activity in primary cultured rat hepatocytes. Bioorg. Med. Chem. Lett. 7(17): 2193–2198.
Recio, M.D.C., Just, M.J., Giner, R.M., Mañez, S., Rios, J.L., and Hostettmann, K. (1995). Anti-inflammatory activity of saikosaponins from Heteromorpha trifoliata. J. Nat. Prod. 58(1): 140–144.
Shimaoka, A., Seo, S., and Minato, H. (1975). Saponins isolated from Bupleurum falcatum L.; components of saikosaponin b. J. Chem. Soc., Perkin Trans. 1(20): 2043–2048.
Tian, R.T., Xie, P.S., and Liu, H.P. (2009). Evaluation of traditional Chinese herbal medicine: Chaihu (Bupleuri Radix) by both high-performance liquid chromatographic and high-performance thin-layer chromatographic fingerprint and chemometric analysis. J. Chromatogr. A 1216(11): 2150–2155.
Tsai, Y.J., Chen, I.L., Horng, L.Y., and Wu, R.T. (2002). Induction of differentiation in rat C6 glioma cells with Saikosaponins. Phytother. Res. 16(2): 117–121.
Vilkhu, K., Mawson, R., Simons, L., and Bates, D. (2008). Applications and opportunities for ultrasound assisted extraction in the food industry—A review. Innovative Food Sci. Emerging Technol. 9(2): 161–169.
Wang, L., and Weller, C.L. (2006). Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol. 17(6): 300–312.
Wang, W., Li, S., Xue, J., Fan, L., Sheng, Z., and Chen, Y. (2010). Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells. Carbohydr. Polym. 80(3): 944–948.
Xin, T., Su, C., Lin, Y., Wang, S., Xu, Z., and Song, J. (2018). Precise species detection of traditional Chinese patent medicine by shotgun metagenomic sequencing. Phytomedicine 47: 40–47.
Xu, L., Tian, J., Song, R., Liu, G., Tian, Y., and Zhang, Z. (2012). LC-MS/MS determination and comparison of saikosaponin a, b2, c, d in crude and processed radix bupleuri by vinegar. J. China Pharm. Univ. 43(4): 334–340.
Yang, F., Dong, X., Yin, X., Wang, W., You, L., and Ni, J. (2017). Radix Bupleuri: a review of traditional uses, botany, phytochemistry, pharmacology, and toxicology. Biomed. Res. Int. 2017: 7597596.
Yu, L., Li, Y., and Guo, X.J. (2009). Simultaneous determination of anthraquinones in Hedyotis diffusa by LC coupled with UV detection. Chromatographia 70(1): 211–215.
Zhao, Y., Wang, Y.J., Zhao, R.Z., and Xiang, F.J. (2016). Vinegar amount in the process affected the components of vinegar-baked Radix Bupleuri and its hepatoprotective effect. BMC Complementary Altern. Med. 16(1): 1–7.
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