Discover the SciOpen Platform and Achieve Your Research Goals with Ease.
Search articles, authors, keywords, DOl and etc.
The root system plays a vital role in plants' ability to absorb water and nutrients. In situ root research offers an intuitive approach to exploring root phenotypes and their dynamics. Deep-learning-based root segmentation methods have gained popularity, but they require large labeled datasets for training. This paper presents an expansion method for in situ root datasets using an improved CycleGAN generator. In addition, spatial-coordinate-based target background separation method is proposed, which solves the issue of background pixel variations caused by generator errors. Compared to traditional threshold segmentation methods, this approach demonstrates superior speed, accuracy, and stability. Moreover, through time-division soil image acquisition, diverse culture medium can be replaced in in situ root images, thereby enhancing dataset versatility. After validating the performance of the Improved_UNet network on the augmented dataset, the optimal results show a 0.63% increase in mean intersection over union, 0.41% in F1, and 0.04% in accuracy. In terms of generalization performance, the optimal results show a 33.6% increase in mean intersection over union, 28.11% in F1, and 2.62% in accuracy. The experimental results confirm the feasibility and practicality of the proposed dataset augmentation strategy. In the future, we plan to combine normal mapping with rendering software to achieve more accurate shading simulations of in situ roots. In addition, we aim to create a broader range of images that encompass various crop varieties and soil types.
Hinsinger P, Brauman A, Devau N, Gérard F, Jourdan C, Laclau J-P, Le Cadre E, Jaillard B, Plassard C. Acquisition of phosphorus and other poorly mobile nutrients by roots. Where do plant nutrition models fail? Plant Soil. 2011;348:29–61.
Lynch JP, Wojciechowski T. Opportunities and challenges in the subsoil: Pathways to deeper rooted crops. J Exp Bot. 2015;66(8):2199–2210.
Zhu H, Zhang LM, Garg A. Investigating plant transpiration-induced soil suction affected by root morphology and root depth. Comput Geotech. 2018;103:26–31.
Dlamini NE, Zhou M. Soils and seasons effect on sugarcane ratoon yield. Field Crop Res. 2022;284:Article 108588.
Chen Y, Zheng J, Yang Z, Xu C, Liao P, Pu S, El-Kassaby YA, Feng J. Role of soil nutrient elements transport on Camellia oleifera yield under different soil types. BMC Plant Biol. 2023;23(1):378.
Liu S, Begum N, An T, Zhao T, Xu B, Zhang S, Deng X, Lam H-M, Nguyen HT, Siddique KH, et al. Characterization of root system architecture traits in diverse soybean genotypes using a semi-hydroponic system. Plants. 2021;10(12):2781.
Aziz AA, Lim KB, Rahman EKA, Nurmawati MH, Zuruzi AS. Agar with embedded channels to study root growth. Sci Rep. 2020;10(1):14231.
Ingram PA, Zhu J, Shariff A, Davis IW, Benfey PN, Elich T. High-throughput imaging and analysis of root system architecture in Brachypodium distachyon under differential nutrient availability. Philos Trans R Soc Lond B Biol Sci. 2012;367(1595):1559–1569.
Bates GH. A device for the observation of root growth in the soil. Nature. 1937;139:966–967.
Koenig C, Wey H, Binkley T. Precision of the XCT 3000 and comparison of densitometric measurements in distal radius scans between XCT 3000 and XCT 2000 peripheral quantitative computed tomography scanners. J Clin Densitom. 2008;11(4):575–580.
Borisjuk L, Rolletschek H, Neuberger T. Surveying the plant’s world by magnetic resonance imaging. Plant J. 2012;70(1):129–146.
Liang Q, Liao H, Yan X. Quantitative analysis of plant root architecture. Chin J Bot. 2007;24(06):695.
Mohamed A, Monnier Y, Mao Z, Lobet G, Maeght J-L, Ramel M, Stokes A. An evaluation of inexpensive methods for root image acquisition when using rhizotrons. Plant Methods. 2017;13:11.
Zhao H, Wang N, Sun H, Zhu L, Zhang K, Zhang Y, Zhu J, Li A, Bai Z, Liu X, et al. RhizoPot platform: A high-throughput in situ root phenotyping platform with integrated hardware and software. Front Plant Sci. 2022;13:Article 1004304.
Abràmoff MD, Magalhães PJ, Ram SJ. Image processing with ImageJ. Biophotonics Int. 2004;11:36–42.
Le Bot J, Serra V, Fabre J, Draye X, Adamowicz S, Pagès L. DART: A software to analyse root system architecture and development from captured images. Plant Soil. 2010;326(1):261–273.
Lobet G, Draye X, Périlleux C. An online database for plant image analysis software tools. Plant Methods. 2013;9(1):38.
Wang T, Rostamza M, Song Z, Wang L, Mcnickle G, Iyer-Pascuzzi AS, Qiu Z, Jin J. SegRoot: A high throughput segmentation method for root image analysis. Comput Electron Agric. 2019;162:845–854.
Badrinarayanan V, Kendall A, Cipolla R. SegNet: A deep convolutional encoder-decoder architecture for image segmentation. IEEE Trans Pattern Anal Mach Intell. 2017;39(12):2481–2495.
Jubery TZ, Carley CN, Singh A, Sarkar S, Ganapathysubramanian B, Singh AK. Using machine learning to develop a fully automated soybean nodule acquisition pipeline (SNAP). Plant Phenomics. 2021;2021:Article 9834746.
Gaggion N, Ariel F, Daric V, Lambert E, Legendre S, Roulé T, Camoirano A, Milone DH, Crespi M, Blein T, et al. ChronoRoot: High-throughput phenotyping by deep segmentation networks reveals novel temporal parameters of plant root system architecture. GigaScience. 2021;10(7):giab052.
Smith AG, Han E, Petersen J, Olsen N, Giese C, Athmann M, Dresbøll DB, Thorup-Kristensen K. RootPainter: Deep learning segmentation of biological images with corrective annotation. New Phytol. 2022;236(2):774–791.
Chen R, Zhao J, Yao X, Jiang S, He Y, Bao B, Luo X, Xu S, Wang C. Generative design of outdoor green spaces based on generative adversarial networks. Buildings. 2023;13(4):1083.
Zhang W, Chen K, Wang J, Shi Y, Guo W. Easy domain adaptation method for filling the species gap in deep learning-based fruit detection. Hortic Res. 2021;119.
Mi J, Gao W, Yang S, Hao X, Li M, Wang M, Zheng L. A method of plant root image restoration based on GAN. IFAC-PapersOnLine. 2019;52(30):219–224.
Zhu L, Liu L, Sun H, Zhang K, Zhang Y, Li A, Bai Z, Wang G, Liu X, Dong H, et al. Low nitrogen supply inhibits root growth but prolongs lateral root lifespan in cotton. Ind Crop Prod. 2022;189:Article 115733.
Zhu L, Liu L, Sun H, Zhang Y, Liu X, Wang N, Chen J, Zhang K, Bai Z, Wang G, et al. The responses of lateral roots and root hairs to nitrogen stress in cotton based on daily root measurements. J Agron Crop Sci. 2022;208(1):89–105.
Xiao S, Liu L, Zhang Y, Sun H, Zhang K, Bai Z, Dong H, Li C. Fine root and root hair morphology of cotton under drought stress revealed with RhizoPot. J Agron Crop Sci. 2020;206(6):679–693.
Zhu L, Li A, Sun H, Li P, Liu X, Guo C, Zhang Y, Zhang K, Bai Z, Dong H, et al. The effect of exogenous melatonin on root growth and lifespan and seed cotton yield under drought stress. Ind Crop Prod. 2023;204:117344.
Yu Q, Wang J, Tang H, Zhang J, Zhang W, Liu L, Wang N. Application of improved UNet and EnglightenGAN for segmentation and reconstruction of in situ roots. Plant Phenomics. 2023;5:0066.
Shen C, Liu L, Zhu L, Kang J, Shao L. High-throughput in situ root image segmentation based on the improved DeepLabv3+ method. Front Plant Sci. 2020;11:Article 576791.
Jia KA, Llbc D, Fz E, Chen SA, Nan W, Ls A. Semantic segmentation model of cotton roots in-situ image based on attention mechanism. Comput Electron Agric. 2021;189:Article 106370.
Gao Y, Li Y, Jiang R, Zhan X, Lu H, Guo W, Yang W, Ding Y, Liu S. Enhancing green fraction estimation in Rice and wheat crops: A self-supervised deep learning semantic segmentation approach. Plant Phenomics. 2023;5:0064.
Li Y, Zhan X, Liu S, Lu H, Jiang R, Guo W, Chapman S, Ge Y, Solan B, Ding Y, et al. Self-supervised plant phenotyping by combining domain adaptation with 3D plant model simulations: Application to wheat leaf counting at seedling stage. Plant Phenomics. 2023;5:0041.
Sahin HM, Miftahushudur T, Grieve B, Yin H. Segmentation of weeds and crops using multispectral imaging and CRF-enhanced U-Net. Comput Electron Agric. 2023;211:Article 107956.
Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0).