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Publishing Language: Chinese

Correlation Between Stomatal Characteristics and Cold Resistance of Brassica napus L.

JunQiang FAN1,2()JunYan WU1,2LiJun LIU2Li MA2Gang YANG1,2YuanYuan PU1,2XueCai LI1,2WanCang SUN1,2()
College of Agronomy, Gansu Agricultural University, Lanzhou 730070
State Key Laboratory of Crop Science in Arid Habitats, Lanzhou 730070
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Abstract

【Objective】

The characteristics of the stomatal movement of winter Brassica napus with different cold resistance in northern China in a low-temperature environment were analyzed, and the relationship between cold resistance and stomatal movement was clarified, which provided a basis for analyzing the cold resistance mechanism of winter Brassica napus and cultivating strong cold resistance varieties.

【Method】

The physiological indexes of 14 winter Brassica napus varieties under semi-lethal temperature and low temperature were determined to judge the difference in cold resistance. At the same time, 12 stomatal indexes of the lower epidermis of the leaves of the plants treated at 24℃ for 12 h, 0℃ for 1 h, and 0℃ for 12 h were determined. Stomatal evaluation indexes significantly related to cold resistance evaluation indexes were screened by correlation analysis.

【Result】

(1) According to the results of semi-lethal temperature determination, the cold resistance of the tested varieties was identified as gau-1 (-8.06)>gau-24 (-7.83)>gau-30 (-7.58)>gau-39 (-7.44)>ts309 (-7.28)>ts312 (-7.08)>nts158 (-6.81)>npz269 (-6.62)>Tianyou 14 (-5.98)>16-2444 (-5.4)>17-2251 (-5.13)>Tianyou 2266 (-4.8)>Tianyou 2238 (-4.6)>Tianyou 2288 ( -4.38 ). According to the comprehensive evaluation value of physiological indexes, the results of cold resistance were gau-1 (0.990)>gau-24 (0.876)>gau-30 (0.693)>gau-39 (0.644)>ts309 (0.534)>ts312 ( 0.463)>nts158 (0.439)>npz269 (0.388)>Tianyou 14 (0.352)>16-2444 (0.307)>17-2251 (0.282)>Tianyou 2266 (0.236)>Tianyou 2238 (0.126)>Tianyou 2288 ( 0.000). The cold resistance measured by semi-lethal temperature was consistent with the comprehensive evaluation results of physiological indexes. (2) 12 stomatal movement-related indicators changed significantly after low-temperature treatment. The correlation analysis showed that there was no significant correlation between the 12 stomatal indexes and the semi-lethal temperature at room temperature and 0℃ after 1 h treatment. After 12 h treatment at 0℃, the stomatal pore length, area of stomatal pore, circumference of stomatal pore, stomatal apparatus length, area of stomatal apparatus, Circumference of stomatal apparatus, and stomatal closure rate were significantly correlated with cold resistance. The semi-lethal temperature was significantly correlated with the comprehensive evaluation value (Z) of stomata, and the correlation coefficient was-0.572.

【Conclusion】

Low-temperature treatment can significantly affect the stomatal movement of winter Brassica napus, and the characteristics of stomatal closure tend to be more obvious with the extension of low-temperature treatment time. At the same time, the relative changes of stomata of different temperature-sensitive varieties were different, and the varieties with strong cold resistance had stronger ability to keep stomata open after low-temperature treatment.

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Scientia Agricultura Sinica
Pages 599-618
Cite this article:
FAN J, WU J, LIU L, et al. Correlation Between Stomatal Characteristics and Cold Resistance of Brassica napus L.. Scientia Agricultura Sinica, 2023, 56(4): 599-618. https://doi.org/10.3864/j.issn.0578-1752.2023.04.002
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