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Publishing Language: Chinese

Identification and Evaluation of Stripe Rust Resistance in 153 Wheat Collections

JingWei ZHOU1, BoWei YE1, PengFei ZHANG1, YuQing ZHANG2, Min HAO1, YuRuo YIN1, Chan YUAN1, ZhiKang LI1, ShunDa LI1, XianChun XIA3, ZhongHu HE3, HongJun ZHANG3(), CaiXia LAN1()
College of Plant Science & Technology, Huazhong Agricultural University/Hubei Hongshan Laboratory, Wuhan 430070
Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430072
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Wheat Improvement Center, Beijing 100081
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Abstract

【Objective】

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), significantly reduced wheat production worldwide. Breeding resistant wheat varieties is currently considered to be one of the most economical and effective ways to control this disease. Understanding the resistance level of Chinese and International Maize and Wheat Improvement Center (CIMMYT) wheat breeding materials and the distribution of known disease resistance genes will greatly helpful for discovering the new resistance resources and improving the utilization efficiency of disease resistance genes.

【Method】

In the present study, we phenotyped 153 wheat breeding lines derived from China and CIMMYT at both seedling against prevalent Chinese Pst races CYR33 and CYR34. In 2018-2019, 2019-2020 and 2020-2021, using the Pst races CYR33 and CYR34 to identify the materials at the adult plant stages in Ezhou, Hubei. In addition, we used the gene-based or closely linked molecular markers of known stripe rust resistance genes Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr29 and YrSP to genotype the whole set of wheat collections.

【Result】

We found 10 lines immune against CYR33 at the seedling stage (IT: 0), including seven Chinese cultivars (Shannong 28, Luomai 163, Shimai 13, Zhongyi 6, Tanmai 98-2, Zhongmai 175, Taishan 21) and three CIMMYT lines (CIM-53, CIM-60 and CIM-71). However, only two cultivars, Tanmai 98-1 and Shannong 102, showed immune to CYR34 at the seedling stage. Based on the three years field tests, we found 64 lines showed highly resistance to stripe rust (final disease severity, FDS≤5%), including seven Chinese cultivars and 57 CIMMYT lines. The molecular marker analysis of known stripe rust resistance genes showed that there were 31, 23, 73, 2, 4, 50 and 2 lines carrying resistance genes Yr9, Yr10, Yr17, Yr18, Yr26, Yr29 and YrSP, respectively. None of any lines had Yr5 and Yr15. Based on the phenotype, only CIM-53 showed immune against two races at both seedling and adult plant stages (IT=0, FDS=0) and it might carry the known stripe rust resistance gene combination of Yr17+Yr29 based on the genotype.

【Conclusion】

A total of 153 wheat collections from China and CIMMYT were showed adult plant resistance to the prevalent Pst races. Among these, Chinese wheat varieties mainly carry Yr9, Yr10 and Yr26, while CIMMYT wheat line mainly carry Yr17, Yr18 and Yr29, indicating that near-immunity resistance of CIMMYT wheat lines due to combinations of 1-2 moderate seedling resistance gene and 2-3 adult plant resistance genes resulting in durable resistance. Therefore, it is very urgent to expand the resistance sources and identify new resistance genes for pyramiding more genes biotechnology methods to develop new wheat varieties with durable rusts resistance and good agronomic traits. This plays an important role for controlling stripe rust in China by improving the resistance level of wheat variety overall.

Keywords

stripe rustseedling resistanceadult plant resistanceresistance genemolecular markerphenotyping of wheat disease

References

[1]
CHEN X M. Epidemiology and control of stripe rust on wheat. Canadian Journal of Plant Pathology, 2005, 27: 314-337.
Crossref Google Scholar
[2]
SU D, LÜ G Q, ZHANG H, PENG X H, PENG H. Analysis of the occurrence characteristics and influencing factors of wheat stripe rust in Henan province from 2019 to 2020. China Plant Protection, 2021, 41(2): 44-46. (in Chinese)
Google Scholar
[3]
XU Y Y, YANG J J, ZHANG Q D, XIE Y L, GU H. Epidemic characteristics and key control strategies of wheat stripe rust in 2020 in Hubei province. China Plant Protection, 2021, 41(2): 100-103. (in Chinese)
Google Scholar
[4]
LI J, DUNDAS I, DONG C, LI G, TRETHOWAN R, YANG Z, HOXHA S, ZHANG P. Identification and characterization of a new stripe rust resistance gene Yr83 on Rye chromosome 6R in wheat. Theoretical and Applied Genetics, 2020, 133(4): 1095-1107.
Crossref Google Scholar
[5]
ZENG Q D, SHEN C, YUAN F P, WANG Q L, WU J H, XUE W B, ZHAN G M. YAO S, CHEN W, HUANG L L, HAN D J, KANG Z S. The resistance evaluation of the Yr genes to the main prevalent pathotypes of Puccinia striiformis f. sp. tritici in China. Acta Phytopathologica Sinica, 2015, 45(6): 641-650. (in Chinese)
Google Scholar
[6]
ELLIS J G, LAGUDAH E S, SPIELMEYER W, DODDS P N. The past, present and future of breeding rust resistant wheat. Frontiers in Plant Science, 2014, 5: 13.
Crossref Google Scholar
[7]
HAN D J, KANG Z S. Current status and future strategy in breeding wheat for resistance to stripe rust in China. Plant Protection, 2018, 44(5): 1-12. (in Chinese)
Google Scholar
[8]
ZHOU Y, HE Z H, ZHANG G S, XIA L Q, CHEN X M, GAO Y C, JING Z B, YU G J. Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agronomica Sinica, 2004, 30(6): 531-535. (in Chinese)
Google Scholar
[9]
HAN D J, WANG Q L, CHEN X M, ZENG Q D, WU J H, XUE W B, ZHAN G M, HUANG L L, KANG Z S. Emerging Yr26-virulent races of Puccinia striiformis f. tritici are threatening wheat production in the Sichuan Basin, China. Plant Disease, 2015, 99(6): 754-760.
Crossref Google Scholar
[10]
LINE R F, QAYOUM A. Virulence, aggressiveness, evolution and distribution of races of Puccinia striiformis (the cause of stripe of wheat) in North America, 1968-1987. Washington DC: U.S. Department of Agriculture, Agricultural Research Service, 1992: 1-44.
[11]
PETERSON R F, CAMPBELL A B, HANNAH A E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 1948, 265): 496-500.
Crossref Google Scholar
[12]
ROELFS A P, SINGH R P, SAARI E E. Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico: CIMMYT Press, 1992: 49-59.
[13]
SHARP P J, KREIS M, SHEWRY P R, GALE M D. Location of beta-amylase sequences in wheat and its relatives. Theoretical and Applied Genetics, 1988, 75: 286-290.
Crossref Google Scholar
[14]
MARCHAL C, ZHANG J P, ZHANG P, FENWICK P, STEUERNAGEL B, ADAMSKI N M, BOYD L, MCINTOSH R, WULFF B B H, BERRY S, LAGUDAH E, UAUY C. BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust. Nature Plants, 2018, 4(9): 662-668.
Crossref Google Scholar
[15]
LIU C, YANG Z J, LI G R, ZENG Z X, ZHANG Y, ZHOU J P, LIU Z H, REN Z L. Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background. Euphytica, 2008, 159(1/2): 249-258.
Crossref Google Scholar
[16]
SHAO Y T, NIU Y C, ZHU L H, ZHAI W X, XU S C, WU L R. Identification of an AFLP marker linked to the stripe rust resistance gene Yr10 in wheat. Chinese Science Bulletin, 2001(17): 60-63.
Crossref Google Scholar
[17]
KLYMIUK V, YANIV E, HUANG L, RAATS D, FATIUKHA A, CHEN S S, FENG L H, FRENKEL Z, KRUGMAN T, LIDZBARSKY G, CHANG W, JAASKELAINEN M J, SCHUDOMA C, PAULIN L, LAINE P, BARIANA H, SELA H, SALEEM K, SORENSEN C K, HOVMOLLER M S, DISTELFELD A, CHALHOUB B, DUBCOVSKY J, KOROL A B, SCHULMAN A H, FAHIMA T. Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family. Nature Communications, 2018, 9: 3735.
Crossref Google Scholar
[18]
SEAH S, BARIANA H, JAHIER J, SIVASITHAMPARAM K, LAGUDAH E S. The introgressed segment carrying rust resistance genes Yr17, Lr37 and Sr38 in wheat can be assayed by a cloned disease resistance gene-like sequence. Theoretical and Applied Genetics, 2001, 102(4): 600-605.
Crossref Google Scholar
[19]
LAGUDAH E S, MCFADDEN H, SINGH R P, HUERTA-ESPINO J, BARIANA H S, SPIELMEYER W. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoretical and Applied Genetics, 2006, 114(1): 21-30.
Crossref Google Scholar
[20]
WANG C M, ZHANG Y P, HAN D J, KANG Z S, LI G P, CAO A Z, CHEN P D. SSR and STS markers for wheat stripe rust resistance gene Yr26. Euphytica, 2008, 159(3): 359-366.
Crossref Google Scholar
[21]
DREISIGACKER S, SEHGAL D, REYESJAIMEZ A E, GARRIDO B L, ZAVALA S M, RIOS C N, MOLLINS J, MALL S. CIMMYT wheat molecular genetics: Laboratory protocols and applications to wheat breeding. Mexico: CIMMYT, 2016: 1-142.
Google Scholar
[22]
KANG Z S, WANG X J, ZHAO J, TANG C L, HUANG L L. Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici.Scientia Agricultura Sinica, 2015, 48(17): 3439-3453. DOI: . (in Chinese)
Crossref Google Scholar
[23]
ZHANG Y, ZHANG X P, LI X P, LI Y. Research progress of the application of molecular marker in genetic breeding of resistance to stripe rust in wheat. Molecular Plant Breeding, 2018, 16(18): 6032-6045. (in Chinese)
Google Scholar
[24]
TONG H W, YANG L J, ZHU Z W, LIU Y K, ZHANG Y Q, FU H H, GAO C B. Agronomic characters and resistance evaluation on wheat germplasms for high rainfall areas from CIMMYT. Hubei Agricultural Sciences, 2009, 48(12): 2950-2953. (in Chinese)
Google Scholar
[25]
WU L, XIA X C, ZHU H Z, LI S Z, ZHENG Y L, HE Z H. Molecular characterization of Lr34/Yr18/Pm38 in 273 CIMMYT wheat cultivars and lines using functional markers. Scientia Agricultura Sinica, 2010, 43(22): 4553-4561. DOI: . (in Chinese)
Crossref Google Scholar
[26]
HAFEEZ A N, ARORA S, GHOSH S, GILBERT D, BOWDEN R L, WULFF B B H. Creation and judicious application of a wheat resistance gene atlas. Molecular Plant, 2021, 14: 1053-1070.
Crossref Google Scholar
[27]
ATHIYANNAN N, ABROUK M, BOSHOFF W H P, CAUET S, RODDE N, KUDRNA D, MOHAMMED N, BETTGENHAEUSER J, BOTHA K S, DERMAN S S, WING R, PRINS R, KRATTINGER S G. Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning. Nature Genetics, 2022, 54: 227-231.
Crossref Google Scholar
[28]
LIU T G, PENG Y L, CHEN W Q, ZHANG Z Y. First detection of virulence in Puccinia triiformis f. sp. tritici in China to resistance genes Yr24 (=Yr26) present in wheat cultivar Chuanmai 42. Plant Disease, 2010, 94(9): 1163.
Crossref Google Scholar
[29]
HUANG L, XIAO X Z, LIU B, GAO L, GONG G S, CHEN W Q, ZHANG M, LIU T G. Identification of stripe Rust resistance genes in common wheat cultivars from the Huang-Huai-Hai region of China. Plant Disease, 2020, 104(6): 1763-1770.
Crossref Google Scholar
[30]
YU R, JIN Y G, WU S S, WU J H, WANG Q L, ZENG Q D, LIU S J, XIA Z H, WANG X J, KANG Z S, HAN D J. Stripe rust resistance of new warieties (Lines) from Huang-Huai valley wheat region in China. Journal of Triticeae Crops, 2020, 40(3): 278-284. (in Chinese)
Google Scholar
[31]
YUAN C, JIANG H, WANG H, LI K, TANG H, LI X, FU D. Distribution, frequency and variation of stripe rust resistance loci Yr10, Lr34/Yr18 and Yr36 in Chinese wheat cultivars. Journal of Genetics and Genomics, 2012, 39(11): 587-592.
Crossref Google Scholar
[32]
LIU W, FRICK M, HUEL R, NYKIFORUK C L, WANG X, GAUDET D A, EUDES F, CONNER R L, KUZYK A, CHEN Q, KANG Z, LAROCHE A. The stripe rust resistance gene Yr10 encodes an evolutionary-conserved and unique CC-NBS-LRR sequence in wheat. Molecular Plant, 2014, 7(12): 1740-1755.
Crossref Google Scholar
[33]
DONG N, CHEN X D, HU T Z, LI G, ZHANG Y J, RU Z G. Molecular detection and evaluation of disease resistance genes of 39 introduced wheat germplasms. Acta Agriculturae Boreali-Sinica, 2018, 33(6): 49-55. (in Chinese)
Google Scholar
[34]
CHEN X M, WANG M N, WAN A M, BAI Q, LI M J, LOPEZ P F, MACCAFERRI M, MASTRANGELO A M, BARNES C W, CRUZ D F C, TENUTA A U, ESMAIL S M, ABDELRHIM A S. Virulence characterization of Puccinia striiformis f. sp. tritici collections from six countries in 2013 to 2020. Canadian Journal of Plant Pathology, 2021, 43: S308-S322.
Crossref Google Scholar
[35]
YU F G, WANG G H, WANG C Y, ZHANG H, LIU X L, TIAN Z R, ZHU J F, CHEN C H, JI W Q, WANG Y J. Evaluation and identification of adult resistance to stripe rust from 400 wheat varieties (lines). Journal of Plant Genetic Resources, 2020, 21(4): 846-854. (in Chinese)
Google Scholar
[36]
DAI M F, MU J M, WANG X T, WANG Q L, YU S Z, HUANG S, ZENG Q D, WU J H, LIU S J, NIE X J, KANG Z S, HAN D J. Screening of stripe rust resistance and molecular detection of Yr genes of wheat germplasms from ICARDA. Journal of Triticeae Crops, 2019, 39(8): 934-940. (in Chinese)
Google Scholar
[37]
HAN D J, ZHANG P Y, WANG Q L, ZENG Q D, WU J H, ZHOU X L, WANG X J, HUANG L L, KANG Z S. Identification and evaluation of resistance to stripe rust in 1980 wheat landraces and abroad germplasm. Scientia Agricultura Sinica, 2012, 45(24): 5013-5023. DOI: . (in Chinese)
Crossref Google Scholar
[38]
MILUS E A, LEE K D, BROWN-GUEDIRA G. Characterization of stripe rust resistance in wheat lines with resistance gene Yr17 and implications for evaluating resistance and virulence. Phytopathology, 2015, 105(8): 1123.
Crossref Google Scholar
[39]
YAO Q, WANG J R, MENG Y, ZHAN G M, HUANG L L, KANG Z S. Virulence and genotypic diversity of wheat stripe rust races CYR32 and CYR33 in China. Journal of Plant Protection, 2018, 45(1): 46-52. (in Chinese)
Google Scholar
[40]
FANG T, CAMPBELL K G, LIU Z Y, CHEN X M, WAN A M, LI S, LIU Z J, CAO S H, CHEN Y H, BOWDEN R L, CARVER B F, YAN L L. Stripe rust resistance in the wheat cultivar Jagger is due to Yr17 and a novel resistance gene. Crop Science, 2011, 51(6): 2455-2465.
Crossref Google Scholar
[41]
YE B W, SINGH R P, YUAN C, LIU D M, RANDHAWA M S, HUERTA-ESPINO J, BHAVANI S, LAGUDAH E, LAN C X. Three co-located resistance genes confer resistance to leaf rust and stripe rust in wheat variety Borlaug 100. The Crop Journal, 2022, 10(2): 490-497.
Crossref Google Scholar
[42]
XUE W B, XU X, MU J M, WANG Q L, WU J H, HUANG L L, KANG Z S, HAN D J. Evaluation of stripe rust resistance and genes in Chinese elite wheat varieties. Journal of Triticeae Crops, 2014, 34(8): 1054-1060. (in Chinese)
Google Scholar
[43]
KRATTINGER S G, LAGUDAH E S, SPIELMEYER W, SINGH R P, HUERTA-ESPINO J, MCFADDEN H, BOSSOLINI E, SELTER L L, KELLER B. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science, 2009, 323: 1360-1363.
Crossref Google Scholar
[44]
LIU J D, YANG E N, XIAO Y G, CHEN X M, WU L, BAI B, LI Z F, GARRY M R, XIA X C, HE Z H. Development, field and molecular characterization of advanced lines with pleiotropic adult-plant resistance in common wheat. Acta Agronomica Sinica, 2015, 41(10): 1472-1480. (in Chinese)
Crossref Google Scholar
[45]
XU M R, LIN R M, WANG F T, FENG J, XU S C. Evaluation of resistance to stripe rust and genetic diversity and detection of resistance genes in 103 wheat cultivars (lines). Scientia Agricultura Sinica, 2020, 53(4): 748-760. DOI: . (in Chinese)
Crossref Google Scholar
[46]
GUAN F N, LONG L, YAO F J, WANG Y Q, JIANG Q T, KANG H Y, JIANG Y F, LI W, DENG M, LI H, CHEN G Y. Evaluation of resistance to stripe rust and molecular detection of important known Yr Gene(s) of 152 Chinese wheat landraces from the Huang-huai-hai. Scientia Agricultura Sinica, 2020, 53(18): 3629-3637. DOI: . (in Chinese)
Crossref Google Scholar
[47]
LILLEMO M, ASALF B, SINGH R P, HUERTA-ESPINO J, CHEN X M, HE Z H, BJORNSTAD A. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theoretical and Applied Genetics, 2008, 116: 1155-1166.
Crossref Google Scholar
[48]
MA J X, ZHOU R H, DONG Y S, WANG L F, WANG X M, JIA J Z. Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica, 2001, 120: 219-226.
Google Scholar
[49]
CHEN X D, WU X J, HU T Z, LI X H, LI G, HOU K X, RU Z G. Evaluation of resistance to stripe rust and molecular detection of resistant genes in wheat germplasms at home and abroad. Journal of Henan Agricultural Sciences, 2019, 48(9): 103-110. (in Chinese)
Google Scholar
[50]
SINGH R P, MUJEEB-KAZI A, HUERTA-ESPINO J. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology, 1998, 88(9): 890-894.
Crossref Google Scholar
[51]
WILLIAM M, SINGH R P, HUERTA-ESPINO J, ISLAS S O, HOISINGTON D. Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology, 2003, 93: 153-159.
Crossref Google Scholar
[52]
KOLMER J A, SU Z, BERNARDO A, BAI G H, CHAO S M. Mapping and characterization of the new adult plant leaf rust resistance gene Lr77 derived from Santa Fe winter wheat. Theoretical and Applied Genetics, 2018, 131: 1553-1560.
Crossref Google Scholar
[53]
LAGUDAH E S. Molecular genetics of race non-specific rust resistance in wheat. Euphytica, 2011, 179: 81-91.
Crossref Google Scholar
[54]
LI W, SONG G Q, LI J H, LI Y L, ZHANG S J, ZHANG R Z, GAO J, GU T T, LI G Y. Molecular detection of four pleiotropic disease resistance genes in wheat. Journal of Triticeae Crops, 2020, 40(4): 395-400. (in Chinese)
Google Scholar
[55]
LAN C X, ROSEWARNE G M, SINGH R P, HERRERA-FOESSEL S A, HUERTA-ESPINO J, BASNET B R, ZHANG Y L, YANG E N. QTL characterization of resistance to leaf rust and stripe rust in the spring wheat line Francolin#1. Molecular Breeding, 2014, 34(3): 789-803.
Crossref Google Scholar
[56]
HAO M, ZHANG L Q, HUANG L, NING S Z, YUAN Z W, JIANG B, YAN Z H, WU B H, ZHENG Y L, LIU D C. Genetic improvement of synthesized hexaploid wheat in breeding. Journal of Plant Genetic Resources, 2022, 23(1): 40-48. (in Chinese)
Google Scholar
[57]
ZHANG H, BIAN Y, GOU X, ZHU B, XU C, QI B, LI N, RUSTGI S, ZHOU H, HAN F, JIANG J, VON WETTSTEIN D, LIU B. Persistent whole-chromosome aneuploidy is generally associated with nascent allohexaploid wheat. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(9): 3447-3452.
Crossref Google Scholar
[58]
ABERKANE H, PAYNE T, KISHI M, SMALE M, AMRI A, JAMORA N. Transferring diversity of goat grass to farmers’ fields through the development of synthetic hexaploid wheat. Food Security, 2020, 12(5): 1017-1033.
Crossref Google Scholar
[59]
ZOU Y C. A new national wheat variety-Chuanmai42. China Agricultural Informatics, 2009(7): 32. (in Chinese)
Google Scholar
Scientia Agricultura Sinica
Volume 57 Issue 1,
January 2024
Pages 18-33
DOI: 10.3864/j.issn.0578-1752.2024.01.003
Cite this article:
ZHOU J, YE B, ZHANG P, et al. Identification and Evaluation of Stripe Rust Resistance in 153 Wheat Collections. Scientia Agricultura Sinica, 2024, 57(1): 18-33. https://doi.org/10.3864/j.issn.0578-1752.2024.01.003
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