Home Scientia Agricultura Sinica Article
PDF (1.7 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Show Outline
Outline
Abstract
Keywords
References
Show full outline
Hide outline
Publishing Language: Chinese

Construction of ms1 Basic Recurrent Populations Adapted to Different Ecological Regions Using Maturity Genes E1 and E2 in Soybean

XueJie HU1,2, LuPing LIU2, FengMin WANG2, YuHua HAN2, BinCheng SUN3, QiBin MA4, ZhiPing HUANG5, Yan FENG2, Qiang CHEN2, ChunYan YANG2, MengChen ZHANG2, Kai ZHANG1(), Jun QIN2()
College of Agronomy and Biotechnology, Hebei Normal University of Science & Technology/Key Laboratory of Crop Stress Biology in Hebei Province, Qinhuangdao 066004, Hebei
Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences/National Soybean Improvement Center Shijiazhuang Sub-Center/Huang-Huai-Hai Key Laboratory of Biology and Genetic Breeding of Soybean, Ministry of Agriculture and Rural Affairs/Hebei Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035
Hulunbuir Institute of Agricultural and Animal Husbandry Sciences, Hulunbuir 162650, Inner Mongolia
College of Agriculture, South China Agricultural University, Guangzhou 510642
Anhui Academy of Agricultural Sciences, Hefei 230031
Show Author Information

Abstract

【Objective】

Soybean is a short day crop that is sensitive to photoperiod, and it maybe lead to premature or late flowering when it is planted in different ecological areas. Therefore, in the application of ms1 (male sterility 1) basic population for recurrent selection in different ecological regions, there are problems such as the flowering time unsynchronization between local donor parents and acceptor sterile plants and low introduction rate. The purpose of this study is to construct ms1 basic recurrent population adapted to three ecological regions for improving the probability of flowering time synchronization between donor parents and acceptor sterile plants and reveal the changes of maturity genes E1 and E2 genotypes and phenotype of each population after two rounds of cross-fertilize for providing evidence for improvement of the flowering and maturity time of soybeans.

【Method】

We used 528 donor parents from different ecological regions and the ms1 basic population as materials. The donor parents were genotyping with the KASP markers of maturity genes E1 and E2 reported by previous research. The donor parents were classified according to E1 and E2 genotypes and mixed with seeds of ms1 basic population respectively, and these populations were planted in different ecological areas according to the suitable genotypes of each region for two rounds of cross-fertilize in two years. Northeast ecological region population was planted in Hulunbuir, Inner Mongolia and Chengde, Hebei, respectively. Huang-Huai-Hai ecological region population was planted in Shijiazhuang, Hebei and Xuchang, Henan. South ecological region population was planted in Guangzhou, Guangdong. Seeds harvested from different ms1 populations were planted in Sanya, Hainan every winter. The flowering and maturity time of donor parents and ms1 basic population were investigated, and the proportions of E1 and E2 genotypes in populations of different region were calculated.

【Result】

According to genotypes of maturity genes E1 and E2, the donor parents were divided into four groups E1E1/E2E2, E1E1/e2e2, e1e1/E2E2 and e1e1/e2e2 with ratios of 12.1%, 65.0%, 19.3%, and 3.6%, respectively. In the ms1 basic population, the late flowering genotype E1E1/E2E2 had the highest proportion (48.6%), and the flowering time of the population was late, mainly concentrated in 45-51 days. After two rounds of import by cross-fertilize, the percentage of target genotype e1e1/e2e2 increased from 33.0% to 51.6% in Hulunbuir of Northeast China, and the percentage of the e1e1/e2e2 genotype increased from 1.6% to 8% in Chengde. The percentage of target genotype e1e1/E2E2 increased from 18% to 23.1% in Shijiazhuang of Huang-Huai-Hai ecological area, and the percentage of E1E1/e2e2 increased from 12.5% to 30% in Xuchang, respectively. The percentage of E1E1/E2E2 remains above 80% in Guangzhou of South ecological region. The proportion of heterozygous genotypes of target imported genotypes was also increasing in the population. After two rounds of cross-fertilize, there were significant differences in flowering time among ms1 populations of different ecological regions, indicating that phenotypes of different populations also changed with the change of genotype of flowering genes.

【Conclusion】

Importing genotype of donor parents into the ms1 population based on their genotypes of flowering genes can increase the frequency of suitable genotypes in each ecological region, construct ms1 basic recurrent populations adapted to different ecological regions, increase the probability of flower time synchronization of local donor parents and acceptor ms1 sterile plants, achieve open pollination, gene aggregation and accumulation in soybean, and enrich the genetic diversity of the population, further improve breeding efficiency.

Keywords

soybeanms1 basic recurrent populationflowering timematurity timeE1E2KASP

References

[1]
ZHAO S J, ZHANG M C, YANG C Y, JIANG C Z, LIU B Q. Selection and breeding of high quality and high protein content soybean variety Jidou 20 and the crucial points of high yield culture techniques. Bulletin of Agricultural Science and Technology, 2010(1): 145-146. (in Chinese)
Google Scholar
[2]
JI Y M, LUO R P, LIAN J F, ZHAO Z G. Creation of new spring soybean germplasm by using soybean male sterile gene ms1 and its application in breeding. Soybean Science & Technology, 2022(1): 9-13. (in Chinese)
Google Scholar
[3]
CHEN D L, CHEN J Q, LI Z D, YANG C J, TAN C Y, ZHU X T. Trait expression and correlation of core germplasm of soybean male sterile (ms1) recurrent selection population in Guizhou. Guizhou Agricultural Sciences, 2018, 46(12): 1-5. (in Chinese)
Google Scholar
[4]
ZHAO Q S, YANG C Y, ZHAO S J, YAN L, SHI X L, LIU L J, ZHAO C C, FENG Y, ZHAO X, ZHANG M C. Application of transgenic herbicide-resistant soybean breeding based on male sterile (ms1) recurrent selection population. Acta Agriculturae Boreali-Sinica, 2016, 31(S1): 112-116. (in Chinese)
Google Scholar
[5]
ZHAO Z G, LUO R P, MA X J, XUN G S. MS1 soybean breeding cycle of group selection and practice. Journal of Anhui Agricultural Sciences, 2011, 39(24): 14555-14556. (in Chinese)
Google Scholar
[6]
FANG X L, SUN X Y, YANG X D, LI Q, LIN C J, XU J, GONG W J, WANG Y F, LIU L, ZHAO L M, LIU B H, QIN J, ZHANG M C, ZHANG C B, KONG F J, LI M N. MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean. Science China-Life Sciences, 2021, 64(9): 1533-1545.
Crossref Google Scholar
[7]
BRIM C A, YOUNG M F. Inheritance of a male-sterile character in soybeans. Crop Science, 1971, 11(4): 564-566.
Crossref Google Scholar
[8]
SONG Q J, WU T X, GAI J Y. Effect of maturity selection in a soybean recurrent population on morphological and agronomic traits and ratio of male-sterile plants. Scientia Agricultura Sinica, 1996, 29(3): 49-54. doi: 10.3864/j.issn.0578-1752.1996-29-03-49-54. (in Chinese)
Google Scholar
[9]
ZHAO S J, ZHANG M C, JIANG C Z, YANG C Y, LIU B Q, CUI Y. Study on quality improvement effect and separate character of soybean male sterile (ms1) recurrent selection population. Scientia Agricultura Sinica, 2006, 39(12): 2422-2427. doi: 10.3864/j.issn.0578-1752.at-2005-6054. (in Chinese)
Google Scholar
[10]
XIA Z J, WATANABE S, YAMADA T, TSUBOKURA Y, NAKASHIMA H, ZHAI H, ANAI T, SATO S, YAMAZAKI T, LÜ S X, WU H Y, TABATA S, HARADA K. Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(32): E2155-E2164.
Crossref Google Scholar
[11]
WATANABE S, XIA Z J, HIDESHIMA R, TSUBOKURA Y, SATO S, YAMANAKA N, TAKAHASHI R, ANAI T, TABATA S, KITAMURA K, HARADA K. A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering. Genetics, 2011, 188(2): 395-407.
Crossref Google Scholar
[12]
WATANABE S, HIDESHIMA R, XIA Z J, TSUBOKURA Y, SATO S, NAKAMOTO Y, YAMANAKA N, TAKAHASHI R, ISHIMOTO M, ANAI T, TABATA S, HARADA K. Map-based cloning of the gene associated with the soybean maturity locus E3. Genetics, 2009, 182(4): 1251-1262.
Crossref Google Scholar
[13]
LIU B H, KANAZAWA A, MATSUMURA H, TAKAHASHI R, HARADA K, ABE J. Genetic redundancy in soybean photoresponses associated with duplication of the phytochrome A gene. Genetics, 2008, 180(2): 995-1007.
Crossref Google Scholar
[14]
BONATO E R, VELLO N A. E6, a dominant gene conditioning early flowering and maturity in soybeans. Genetics and Molecular Biology, 1999, 22(2): 229-232.
Crossref Google Scholar
[15]
FANG C, LIU J, ZHANG T, SU T, LI S C, CHENG Q, KONG L P, LI X M, BU T T, LI H Y, DONG L D, LU S J, KONG F J, LIU B H. A recent retrotransposon insertion of J caused E6 locus facilitating soybean adaptation into low latitude. Journal of Integrative Plant Biology, 2021, 63(6): 995-1003.
Crossref Google Scholar
[16]
COBER E R, VOLDENG H D. A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T. Crop Science, 2001, 41(3): 698-701.
Crossref Google Scholar
[17]
COBER E R, MOLNAR S J, CHARETTE M, VOLDENG H D. A new locus for early maturity in soybean. Crop Science, 2010, 50(2): 524-527.
Crossref Google Scholar
[18]
ZHAO C, TAKESHIMA R, ZHU J H, XU M L, SATO M, WATANABE S, KANAZAWA A, LIU B H, KONG F J, YAMADA T, ABE J. A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog. BMC Plant Biology, 2016, 16: 20.
Crossref Google Scholar
[19]
SAMANFAR B, MOLNAR S J, CHARETTE M, SCHOENROCK A, DEHNE F, GOLSHANI A, BELZILE F, COBER E R. Mapping and identification of a potential candidate gene for a novel maturity locus, E10, in soybean. Theoretical and Applied Genetics, 2017, 130(2): 377-390.
Crossref Google Scholar
[20]
LU S J, DONG L D, FANG C, LIU S L, KONG L P, CHENG Q, CHEN L Y, SU T, NAN H Y, ZHANG D, ZHANG L, WANG Z J, YANG Y Q, YU D Y, LIU X L, YANG Q Y, LIN X Y, TANG Y Y, ZHAO X H, YANG X Q, TIAN C G, XIE Q G, LI X, YUAN X H, TIAN Z X, LIU B H, WELLER J L, KONG F J. Stepwise selection on homeologous PRR genes controlling flowering and maturity during soybean domestication. Nature Genetics, 2020, 52(4): 428-436.
Crossref Google Scholar
[21]
DONG L D, LI S C, WANG L S, SU T, ZHANG C B, BI Y D, LAI Y C, KONG L P, WANG F, PEI X X, LI H Y, HOU Z H, DU H P, DU H, LI T, CHENG Q, FANG C, KONG F J, LIU B H. The genetic basis of high-latitude adaptation in wild soybean. Current Biology, 2023, 33(2): 252-262.
Crossref Google Scholar
[22]
DONG L D, CHENG Q, FANG C, KONG L P, YANG H, HOU Z H, LI Y L, NAN H Y, ZHANG Y H, CHEN Q S, ZHANG C B, KOU K, SU T, WANG L S, LI S C, LI H Y, LIN X Y, TANG Y, ZHAO X H, LU S J, LIU B H, KONG F J. Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes. Molecular Plant, 2022, 15(2): 308-321.
Crossref Google Scholar
[23]
LI H Y, DU H P, HE M L, WANG J H, WANG F, YUAN W J, HUANG Z R, CHENG Q, GOU C J, CHEN Z, LIU B H, KONG F J, FANG C, ZHAO X H, YU D Y. Natural variation of FKF1 controls flowering and adaptation during soybean domestication and improvement. The New Phytologist, 2023, 238(4): 1671-1684.
Crossref Google Scholar
[24]
LI H Y, DU H P, HUANG Z R, HE M L, KONG L P, FANG C, CHEN L Y, YANG H, ZHANG Y H, LIU B H, KONG F J, ZHAO X H. The AP2/ERF transcription factor TOE4b regulates photoperiodic flowering and grain yield per plant in soybean. Plant Biotechnology Journal, 2023, 21(8): 1682-1694.
Crossref Google Scholar
[25]
DONG L D, FANG C, CHENG Q, SU T, KOU K, KONG L P, ZHANG C B, LI H Y, HOU Z H, ZHANG Y H, CHEN L Y, YUE L, WANG L S, WANG K, LI Y L, GAN Z R, YUAN X H, WELLER J L, LU S J, KONG F J, LIU B H. Genetic basis and adaptation trajectory of soybean from its temperate origin to tropics. Nature Communications, 2021, 12(1): 5445.
Crossref Google Scholar
[26]
KOU K, YANG H, LI H Y, FANG C, CHEN L Y, YUE L, NAN H Y, KONG L P, LI X M, WANG F, WANG J H, DU H P, YANG Z Y, BI Y D, LAI Y C, DONG L D, CHENG Q, SU T, WANG L S, LI S C, HOU Z H, LU S J, ZHANG Y H, CHE Z J, YU D Y, ZHAO X H, LIU B H, KONG F J. A functionally divergent SOC1 homolog improves soybean yield and latitudinal adaptation. Current Biology, 2022, 32(8): 1728-1742.
Crossref Google Scholar
[27]
LU S J, ZHAO X H, HU Y L, LIU S L, NAN H Y, LI X M, FANG C, CAO D, SHI X Y, KONG L P, SU T, ZHANG F G, LI S C, WANG Z, YUAN X H, COBER E R, WELLER J L, LIU B H, HOU X L, TIAN Z X, KONG F J. Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield. Nature Genetics, 2017, 49(5): 773-779.
Crossref Google Scholar
[28]
LI X M, FANG C, YANG Y Q, LV T X, SU T, CHEN L Y, NAN H Y, LI S C, ZHAO X H, LU S J, DONG L D, CHENG Q, TANG Y, XU M L, ABE J, HOU X L, WELLER J L, KONG F J, LIU B H. Overcoming the genetic compensation response of soybean florigens to improve adaptation and yield at low latitudes. Current Biology, 2021, 31(17): 3755-3767.
Crossref Google Scholar
[29]
TSUBOKURA Y, WATANABE S, XIA Z J, KANAMORI H, YAMAGATA H, KAGA A, KATAYOSE Y, ABE J, ISHIMOTO M, HARADA K. Natural variation in the genes responsible for maturity loci E1, E2, E3 and E4 in soybean. Annals of Botany, 2014, 113(3): 429-441.
Crossref Google Scholar
[30]
JIANG B J, NAN H Y, GAO Y F, TANG L L, YUE Y L, LU S J, MA L M, CAO D, SUN S, WANG J L, WU C X, YUAN X H, HOU W S, KONG F J, HAN T F, LIU B H. Allelic combinations of soybean maturity Loci E1, E2, E3 and E4 result in diversity of maturity and adaptation to different latitudes. PLoS ONE, 2014, 9(8): e106042.
Crossref Google Scholar
[31]
LIU L P, SONG W W, WANG L W, SUN X G, QI Y P, WU T T, SUN S, JIANG B J, WU C X, HOU W S, NI Z F, HAN T F. Allele combinations of maturity genes E1-E4 affect adaptation of soybean to diverse geographic regions and farming systems in China. PLoS ONE, 2020, 15(7): e0235397.
Crossref Google Scholar
[32]
CAO D, TAKESHIMA R, ZHAO C, LIU B H, JUN A, KONG F J. Molecular mechanisms of flowering under long days and stem growth habit in soybean. Journal of Experimental Botany, 2017, 68(8): 1873-1884.
Crossref Google Scholar
[33]
SONG W W, LIU L P, SUN S, WU T T, ZENG H Y, TIAN S Y, SUN B C, LI W B, LIU L J, WANG S M, XING H, ZHOU X A, NIAN H, LU W C, HAN X Z, WANG S Y, CHEN W Y, GUO T, SONG X Q, TIAN Z Y, CHENG Y X, SONG S H, FU L S, WANG H C, LUO R P, LIU X Y, LIU Q, ZHANG G H, LU S H, XU R, LI S Z, LU W G, ZHANG Q, WANG Z B, JIANG C G, SHEN W L, ZHANG M R, ZHU D H, WANG R Z, CHEN Y, WANG T J, ZHU X T, ZHAN Y, JIANG B J, XU C L, YUAN S, HOU W S, GAI J Y, WU C X, HAN T F. Precise classification and regional delineation of maturity groups in soybean cultivars across China. European Journal of Agronomy, 2023, 151: 126982.
Crossref Google Scholar
[34]
XIA Z J. Research progresses on photoperiodic flowering and maturity genes in soybean (Glycine max Merr.). Acta Agronomica Sinica, 2013, 39(4): 571-579. (in Chinese)
Crossref Google Scholar
[35]
DENG Y Y, ZHAO S J, YAN L, YANG C Y, LIU B Q, DI R, DONGFANG Y, ZHANG M C. Effect of orthoselection on agronomic traits and genetic base of a male sterile soybean recurrent population. Soybean Science, 2015, 34(4): 548-554. (in Chinese)
Google Scholar
Scientia Agricultura Sinica
Volume 57 Issue 17,
September 2024
Pages 3305-3317
DOI: 10.3864/j.issn.0578-1752.2024.17.001
Cite this article:
HU X, LIU L, WANG F, et al. Construction of ms1 Basic Recurrent Populations Adapted to Different Ecological Regions Using Maturity Genes E1 and E2 in Soybean. Scientia Agricultura Sinica, 2024, 57(17): 3305-3317. https://doi.org/10.3864/j.issn.0578-1752.2024.17.001
Metrics & Citations  
Article History
Copyright
Return