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Research Article | Open Access

Green Synthesis of Piperine/Triton X-100/Silver Nanoconjugates: Antimicrobial Activity and Cytotoxicity

Muthusamy Prabakaran1Vellaikannu Kalaiarasi2Periakaruppan Nithya1Mani Gajendiran3()Susaimanickam Arul Antony1()
PG and Research Department of Chemistry, Presidency College, Chennai-600005, India
Department of Chemistry, Karpaga Vinayaga College of Engineering and Technology, Palayanoor, Maduranthakam, Chennai- 603308, India
Department of Chemistry, Vels University (VISTAS), Pallavaram, Chennai-600117, India
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Abstract

Silver nanoparticles (AgNPs) were synthesized through a green chemical approach using the piperine isolated from black pepper. The physicochemical properties of Triton X-100 coated silver nanoparticles (Triton X-100/Ag NPs) were well characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and energy dispersive X-ray spectroscopy (EDS). The TEM images confirmed the spherical shape of silver nanoparticles. The powder X-ray diffraction analysis revealed the silver nanoparticles exhibiting face-centered cubic (fcc) crystal structure with an average crystallite size of 15 nm. The cytotoxicity effects on HepG2 cells were also evaluated with a series of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. The effective toxic concentration of Triton X-100/piperine with silver nanoparticles was too low to damage HepG2 cells. The antibacterial activity results showed that the Triton X-100/piperine/AgNPs efficiently inhibited the two bacteria namely S. aureus and E. coli. The Triton X-100/piperine/AgNPs nanoconjugates were effective in inhibiting the growth of both gram-positive and gram-negative bacteria S. aureus and E. coli.

References

[1]

Y. Cheng, F. Wang, C. Fang, et al., Preparation and characterization of size and morphology controllable silver nanoparticles by citrate and tannic acid combined reduction at a low temperature. Journal of Alloys and Compounds, 2016, 658: 684-688.

[2]

E.A. Jun, K.M. Lim, K. Kim, et al., Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity. Nanotoxicology, 2011, 5: 157-197.

[3]

F. Franck, K.S. Morley, W. Ben, et al., Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection? Journal of Antimicrobial Chemotherapy, 2004, 54: 1019-1024.

[4]

C. Dipankar, S. Murugan, The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. Colloids and Surfaces B: Biointerfaces, 2012, 98: 112-119.

[5]

N. Asare, C. Instanes, W.J. Sandberg, et al., Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology, 2012, 291: 65-72.

[6]

P.W. Li, T.H. Kuo, J.H. Chang, et al., Induction of cytotoxicity and apoptosis in mouse blastocysts by silver nanoparticles. Toxicology Letters, 2010, 197: 82-87.

[7]

B. Liu, X. Li, C. Zheng, et al., Facile and green synthesis of silver nanoparticles in quaternized carboxymethyl chitosan solution. Nanotechnology, 2013, 24: 235601.

[8]

M. Ghaedi, M. Youse nejad, M. Safarpoor, et al., Rosmarinus officinalis leaf extract mediated green synthesis of silver nanoparticles and investigation of its antimicrobial properties. Journal of Industrial and Engineering Chemistry, 2015, 31: 167-172.

[9]

A.A. Kajani, A.K. Bordbar, S.H. Zarkesh Esfahani, et al., Green synthesis of anisotropic silver nanoparticles with potent anticancer activity using Taxus baccata extract. RSC Advances, 2014, 4: 61394-61403.

[10]

K. Reena, P. Balashanmugam, M. Gajendiran, et al., Synthesis of Leucas Aspera Extract Loaded Gold-PLA-PEG-PLA Amphiphilic Copolymer Nanoconjugates: In Vitro Cytotoxicity and Anti-Inflammatory Activity Studies. Journal of Nanoscience and Nanotechnology, 2016, 16: 4762-4770.

[11]

K. Reena, M. Gajendiran, M. Prabakaran, et al., Caffeine-loaded gold nanoparticles conjugated with PLA-PEG-PLA copolymer for in vitro cytotoxicity and anti-inflammatory activity. Journal of Industrial and Engineering Chemistry, 2017, 51: 113-121.

[12]

K. Reena, M. Prabakaran, B. Leeba, et al., Green Synthesis of Pectin-Gold-PLA-PEG-PLA Nanoconjugates: In Vitro Cytotoxicity and Anti-Inflammatory Activity, Journal of Nanoscience and Nanotechnology, 2017, 17: 4549-4557.

[13]

K. Wei, W. Li, K. Koike, et al., New amide alkaloids from the roots of Piper nigrum. Journal of Natural Products, 2004, 67: 1005-1009.

[14]

K. Wei, W. Li, K. Koike, et al., Nigramides A−S, Dimeric Amide Alkaloids from the Roots of Piper nigrum. Journal of Organic Chemistry, 2005, 70, 1164-1176.

[15]

K. Shanmugapriya, P.S. Saravana, H. Payal, et al., Antioxidant potential of pepper (Piper nigrum Linn.) leaves and its antimicrobial potential against some pathogenic microbes. Indian Journal of Natural Products Resources, 2012, 3: 570-577.

[16]
B.J. Hudson, Food antioxidants (Elsevier and applied science series). Springer, 1990: 65-98.
[17]

R. Augustine, N. Kalarikkal, S. Thomas et al., A facile and rapid method for the black pepper leaf mediated green synthesis of silver nanoparticles and the antimicrobial study. Applied Nanoscience, 2014, 4: 809-818.

[18]

B.L.V. Prasad, S.K. Arumugam, T. Bala, et al., Solvent-adaptable silver nanoparticles. Langmuir, 2005, 21: 822-826.

[19]

A. Kumar, S. Mandal, P.R. Selvakannan, et al., Investigation into the Interaction between Surface-Bound Alkylamines and Gold Nanoparticles. Langmuir, 2003, 19: 6277-6282.

[20]

Z. Yan, R. Bao, C.Z. Dinu, et al., Laser ablation induced agglomeration of Cu nanoparticles in sodium dodecyl sulfate aqueous solution. Journal of Optoelectronics and Advanced Materials, 2010, 12: 437-439.

[21]

F.D. Pelle, A. Scroccarello, M. Sergi, et al., Simple and rapid silver nanoparticles based antioxidant capacity assays: Reactivity study for phenolic compounds. Food Chemistry, 2018, 256: 342-349.

[22]

S.V. Kumar, A.P. Bafana, P. Pawar, et al., High conversion synthesis of < 10 nm starch-stabilized silver nanoparticles using microwave technology. Scientific Reports, 2018, 8: 5106.

[23]

I.A. Holder, S.T. Boyce, Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns, 1994, 20: 426-429.

[24]

Z. Wang, J. Chen, P. Yang et al., Biomimetic synthesis of gold nanoparticles and their aggregates using a polypeptide sequence. Applied Organometallic Chemistry, 2007, 21: 645-651.

[25]

Y. Wang, X. He, K. Wang, et al., Barbated Skullcup herb extract-mediated biosynthesis of gold nanoparticles and its primary application in electrochemistry. Colloids and Surfaces B: Biointerfaces, 2009, 73: 75-79.

[26]

S.W.P. Wijnhoven, W.J.G. M. Peijnenburg, C.A. Herberts, et al., Nano-silver - a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology, 2009, 3: 109-138.

[27]

S.S. Yudha, D. Notriawan, E. Angasa, et al., Green synthesis of silver nanoparticles using aqueous rinds extract of Brucea javanica (L.) Merr at ambient temperature. Materials Letters, 2013, 97: 181-183.

[28]

N. Asare, C. Instanes, W.J. Sandberg, et al., Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology, 2012, 291: 65-72.

[29]

P.W. Li, T.H. Kuo, J.H. Chang, et al., Induction of cytotoxicity and apoptosis in mouse blastocysts by silver nanoparticles. Toxicology Letters, 2010, 197: 82-87.

[30]

S. Hackenberg, A. Scherzed, M. Kessler, et al., Silver nanoparticles: evaluation of DNA damage, toxicity and functional impairment in human mesenchymal stem cells. Toxicology Letters, 2011, 201: 27-33.

Nano Biomedicine and Engineering
Pages 141-148
Cite this article:
Prabakaran M, Kalaiarasi V, Nithya P, et al. Green Synthesis of Piperine/Triton X-100/Silver Nanoconjugates: Antimicrobial Activity and Cytotoxicity. Nano Biomedicine and Engineering, 2018, 10(2): 141-148. https://doi.org/10.5101/nbe.v10i2.p141-148
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