AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Biomedicine and Engineering Article
PDF (5.5 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

Multifunctional Silver Nanoparticles by Fruit Extract of Terminalia belarica and their Therapeutic Applications: A 3-in-1 System

Sucharitha Kumaram Venkata1,†( ), Susmila Aparna Gaddam2,†( ), Venkata Subbaiah Kotakadi3,†( ), Divi Venkata Ramana Sai Gopal2,3
Department of Home Science, Sri Venkateswara University Tirupati-517502, Andhra Pradesh, India
Department of Virology, Sri Venkateswara University Tirupati-517502, Andhra Pradesh, India
DDST-PURSE Centre, Sri Venkateswara University Tirupati-517502, Andhra Pradesh, India

†Authors contributed equally to the work.

Show Author Information

Abstract

The use of nanoparticles in treating dreadful diseases like cancer is the emerging field of research in cancer therapy. In the present investigation, the green biosynthesis of silver nanoparticles (AgNPs) with aqueous fruit extract of Terminalia belarica has been carried out, and ultraviolet-visible spectroscopy (UV-Vis) analysis was done which revealed an intense surface plasmon resonance (SPR) band at 430 nm, thus confirming the formation of Tb-AgNPs. The AgNPs were further characterized by Fourier transform-infrared spectroscopy (FTIR), showing the reduction of silver nitrate into Tb-AgNPs by the reduction of different functional groups such as hydroxyl, phenols, stretch of aldehydes, alkenes and aromatics. Transmission electron microscopy (TEM) and diffraction light scattering (DLS) study showed that the nanoparticles were round in shape with an average size of 46.5 nm. The atomic force microscopy (AFM) analysis also revealed similar results to facilitate that the AgNPs were round in shape, and the size was calculated by Z-coloration method. Furthermore, the X-ray diffraction (XRD) data confirmed that Tb-AgNPs were crystalline with face centered cubic (fcc) structure and were very stable with −29.1 mV Zeta potential. Tb-AgNPs showed efficient free radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl, hydrogen peroxide and nitric oxide. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method was the best among the three antioxidant methods with the half maximal inhibitory concentration (IC50) value of 47.25 ± 0.17. Tb-AgNPs also showed superior and efficient antibacterial activity greater than the control antibiotic, and showed effective anti-proliferative and cytotoxic effect on human breast cancer cells with IC50 value of 73.18 µg/mL. The biosynthesized Tb-AgNPs with multifunctional properties could be employed as a source for the exploration of novel therapeutic antioxidant, antibacterial and anticancer agent.

Keywords

Green synthesis Terminalia belaricaSpectral characterizationAFM Z coloration analysisAntioxidant activityAntimicrobial activityAnticancer activity

References

[1]

K.N. Thakkar, S.S. Mhatre, and R.Y. Parikh, Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine. 2009, 6: 257-262.
Crossref Google Scholar
[2]

M.A. Dar, A. Ingle, and M. Rai, Enhanced antimicrobial activity of silver nanoparticles synthesized by Cryphonectria sp. evaluated singly and in combination with antibiotics. Nanomedicine: Nanotechnology, Biology and Medicine. 2013, 9: 105-110.
Crossref Google Scholar
[3]

M.S. Akhtar, J. Panwar, and Y.S. Yun, Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustain. Chem. Eng., 2013, 1: 591-602.
Crossref Google Scholar
[4]

N. Vaidyanathan, K. Kalishwaralal, S. Gopalram, et al., Nanosilver——the burgeoning therapeutic molecule and its green synthesis. Biotechnol. Adv., 2009, 27: 924-937.
Crossref Google Scholar
[5]

D. Hebbalalu, J. Lalley, M.N. Nadagouda et al., Greener techniques for the synthesis of silver nanoparticles using plant extracts, enzymes, bacteria, biodegradable polymers, and microwaves. ACS Sustain. Chem. Eng., 2013, 1: 703-712
Crossref Google Scholar
[6]

O.V. Kharissova, H.V. Dias, B.I. Kharisov, et al., The greener synthesis of nanoparticles. Trends Biotechnol., 2013, 31: 240-248.
Crossref Google Scholar
[7]

M.C. Moulton, L.K. Braydich-Stolle, M.N. Nadagouda et al., Synthesis, characterization and biocompatibility of "green" synthesized silver nanoparticles using tea polyphenols. Nanoscale, 2010, 2: 763-770.
Crossref Google Scholar
[8]

M.N. Nadagouda, R.S. Varma, Green synthesis of silver and palladium nanoparticles at room temperature using coffee and tea extract. Green Chem., 2008, 10: 859-862.
Crossref Google Scholar
[9]

K.B. Narayanan, N. Sakthivel, Biological synthesis of metal nanoparticles by microbes. Adv. Colloid Interface Sci., 2010, 156(1-2): 1-13
Crossref Google Scholar
[10]

V.S. Kotakadi, Y. Subba Rao, S.A. Gaddam, et al., Simple and rapid biosynthesis of stable silver nanoparticles using dried leaves of Catharanthus roseus. Linn. G. Donn and its anti microbial activity. Colloids Surf. B: Biointerfaces. 2013, 105: 194-198.
Crossref Google Scholar
[11]

V.S. Kotakadi, Y. Subba Rao, Gaddam SA, et al., Biofabrication of silver nanoparticles by Andrographis paniculata. Eur J Med Chem., 2013, 73: 135-140.
Crossref Google Scholar
[12]

V.S. Kotakadi, S.A. Gaddam, S.K. Venkata, et al., Ficus fruit-mediated biosynthesis of silver nanoparticles and their antibacterial activity against antibiotic resistant E. coli strains. Curr Nanosci, 2015, 11(4): 527-538.
Crossref Google Scholar
[13]

V.S. Kotakadi, S.A. Gaddam, S.K. Venkata, et al., New generation of bactericidal silver nanoparticles against different antibiotic resistant Escherichia coli strains. Appl Nanosci, 2015, 5(7): 847-855.
Crossref Google Scholar
[14]

V.S. Kotakadi, S.A. Gaddam, S.K. Venkata, et al., Biofabrication and spectral characterization of silver nanoparticles and their cytotoxic studies on human CD34 +ve stem cells. 3 Biotech, 2016, 6(2): 216.
Crossref Google Scholar
[15]

V.A. Litvin, B.F. Minaev, Spectroscopy study of silver nanoparticles fabrication using synthetic humic substances and their antimicrobial activity. Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, 2013, 108: 115-122.
Crossref Google Scholar
[16]

K. Chaloupka, Y. Malam, and A.M. Seifalian, Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol, 2010, 28: 580-588.
Crossref Google Scholar
[17]

P. Singh, Y.J. Kim, and D.C. Yang. A strategic approach for rapid synthesis of gold and silver nanoparticles by Panax ginseng leaves. Artif. Cells Nanomed. Biotechnol., 2016, 44: 1949-1957.
Crossref Google Scholar
[18]

L. Xin, J. Wang, G. Fan, et al., Oxidative stress and mitochondrial injury-mediated cytotoxicity induced by silver nanoparticles in human A549 and HepG2 cells. Environ. Toxicol., 2015, 12: 1691-1699.
Crossref Google Scholar
[19]

D.R. Nogueira, C.M.B. Rolim, and A.A. Farooqi, Nanoparticle induced oxidative stress in cancer cells: adding new pieces to an incomplete jigsaw puzzle. Asian Pac. J. Cancer Prev., 2014, 15: 4739-4743.
Crossref Google Scholar
[20]

V. Castro Aceituno, S. Ahn, S.Y. Simu, et al., Silver nanoparticles from Dendropanax morbifera Léveille inhibit cell migration, induce apoptosis, and increase generation of reactive oxygen species in A549 lung cancer cells. In Vitro Cell Dev Biol Anim., 2016, 52: 1012-1019.
Crossref Google Scholar
[21]

A.K. Mittal, A. Kaler, and U.C. Banerjee, Free radical scavenging and antioxidant activity of silver nanoparticles synthesized from flower extract of Rhododendron dauricum. Nano BioMed Eng., 2006, 4: 118-124.
Crossref Google Scholar
[22]

E. Pick, D. Mizel, Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods, 1981, 46: 211-226.
Crossref Google Scholar
[23]

A. Sousa, I.C.F.R. Ferreira, L. Barros, et al., Effect of solvent and extraction temperatures on the antioxidant potential of traditional stoned table olives ''alcaparras". Food Sci Technol-LWT., 2008, 41: 739-745.
Crossref Google Scholar
[24]

T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983, 65: 55-63.
Crossref Google Scholar
[25]
Indian Drug Manufacturer's Association, Indian herbal pharmacopoeia revised new edition 2002. Indian Drug Manufacturer's Association, Mumbai. 2002: 429-438.
[26]
A.S. Saroya, Herbalism phytochemistry and ethanopharmacology. Science Publishers, 2011: 357-361.
[27]

S.N. Motamarri, M. Karthikeyan, M. Kannan, et al., Terminalia belerica. Roxb -- a phytopharmacological review. International Journal of Research in Pharmaceutical and Biomedical Sciences, 2012, 3: 96-99.
Google Scholar
[28]
U. Kreibig, M. Vollmer, Optical properties of metal clusters. Springer, 1995: 1-532.
Crossref
[29]

T.C. Prathna, N. Chandrasekaran, M. Ashok, et al., Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids Surf B, 2011, 82: 152-159.
Crossref Google Scholar
[30]

J.J. Mock, M. Barbic, D.R. Smith, et al., Shape effects in plasmon resonance of individual colloidal silver nanoparticles. J. Chem. Phys. , 2002, 116: 6755-6759.
Crossref Google Scholar
[31]

K. Vijayaraghavan, S.P. Kamala Nalini, N. Udaya Prakash, et al., One step green synthesis of silver nano/microparticles using extracts of Trachyspermum ammi and Papaver somniferum. Colloids Surf. B, 2012, 94: 114-117.
Crossref Google Scholar
[32]

P. Sivalingam, J.J. Antony, D. Siva, et al., Mangrove Streptomyces sp. BDUKAS10 as nanofactory for fabrication of bactericidal silver nanoparticles. Colloids Surf. B, 2012, 98: 12-17.
Crossref Google Scholar
[33]

A. Panacek, L. Kvítek, R. Prucek, et al., Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B, 2006, 110: 16248-16253.
Crossref Google Scholar
[34]

A.R. Shahverdi, S. Minaeian, H.R. Shahverdi, et al., Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: A novel biological approach. Process Biochem., 2007, 42: 919-923.
Crossref Google Scholar
[35]

R. Vijayalakshmi, R. Ravindhran, Comparative fingerprint and extraction yield of Diospyrus ferrea (willd. ) Bakh. root with phenol compounds (gallic acid), as determined by UV-Vis and FT-IR spectroscopy. Asian Pac J Trop Biomed, 2012, 2: 1367.
Crossref Google Scholar
[36]

O.R. Sanchez, P. Balderas, N. Flores, et al., Gamma irradiation induced degradation of orange peels. Energies, 2012, 5: 3051-3063.
Crossref Google Scholar
[37]

P. Sandeep, C. Gunjan, P. Jayasinh, et al., Instant green synthesis of silver-based herbo-metallic colloidal nanosuspension in Terminalia bellirica fruit aqueous extract for catalytic and antibacterial applications. 3 Biotech, 2017, 7: 1-12.
Crossref Google Scholar
[38]

S.A. Gaddam, V.S. Kotakadi, Y. Subba Rao, et al., Efficient and robust biofabrication of silver nanoparticles by cassia alata leaf extract and their antimicrobial activity. J Nanostruct Chem, 2014, 4: 82.
Crossref Google Scholar
[39]

V.A. Litvin, R.L. Galagan, and B.F. Minaev, Kinetic and mechanism formation of silver nanoparticles coated by synthetic humic substances. Colloids & Surfaces A: Physicochem. Eng. Aspects, 2012, 414: 234-243.
Crossref Google Scholar
[40]

A.K. Suresh, M.J. Doktycz, W. Wang, et al., Monodispersed biocompatible silver sulfide nanoparticles: facile extracellular biosynthesis using the γ-proteobacterium, Shewanella oneidensis. Acta Biomater, 2011, 7: 4253-4258.
Crossref Google Scholar
[41]

P. Rauwel, S. Kuunal, S. Ferdov, et al., A review on the green synthesis of silver nanoparticles and their morphologies studied via TEM. Adv Mater Sci Eng, 2015.
Crossref Google Scholar
[42]

S. Ghosh, S. Patil, M. Ahire et al., Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. Int J Nanomedicine, 2012, 7: 483-496.
Crossref Google Scholar
[43]

C.G. Kumar, Y. Poornachandra, Biodirected synthesis of Miconazole-conjugated bacterial silver nanoparticles and their application as antifungal agents and drug delivery vehicles. Colloids Surf B Biointerfaces, 2015, 125: 110-119.
Crossref Google Scholar
[44]

V.R. Netala, S. Bukke, L. Domdi, et al., Biogenesis of silver nanoparticles using leaf extract of Indigofera hirsuta L. and their potential biomedical applications (3-in-1 system). Artificial Cells, Nanomedicine and Biotechnology, 2018, 7: 1-11.
Crossref Google Scholar
[45]

B. Ajitha, Y.A. Reddy, and P.S. Reddy, Biosynthesis of silver nanoparticles using Momordica charantia leaf broth: Evaluation of their innate antimicrobial and catalytic activities. J Photochem Photobiol B Biol, 2015, 146: 1.
Crossref Google Scholar
[46]

P. Dauthal, M. Mukhopadhyay, Biosynthesis of palladium nanoparticles using Delonix regia leaf extract and its catalytic activity for nitro-aromatics hydrogenation. Ind Eng Chem Res, 2013, 52: 18131-18139.
Crossref Google Scholar
[47]

C.H. Ramamurthy, M. Padma, and I.D. Samadanam, The extra cellular synthesis of gold and silver nanoparticles and their free radical scavenging and antibacterial properties. Colloids Surf B Biointerfaces, 2013, 102: 808-815.
Crossref Google Scholar
[48]

W. He, Y.T. Zhou, W.G. Wamer, et al., Mechanisms of the pH dependent generation of hydroxyl radicals and oxygen induced by Ag nanoparticles. Biomaterials, 2012, 33: 7547-7555.
Crossref Google Scholar
[49]

R. Mata, J.R. Nakkala, and S.R. Sadras, Biogenic silver nanoparticles from Abutilon indicum: their antioxidant, antibacterial and cytotoxic effects in vitro. Coll Surf B Biointerfaces, . 2015, 128: 276-286.
Crossref Google Scholar
[50]

H.S.A. Al-Shmgani, W.H. Mohammed, G.M. Sulaiman, et al., Biosynthesis of silver nanoparticles from Catharanthus roseus leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities. Artificial Cells, Nanomedicine and Biotechnology, 2017, 45: 1234-1240.
Crossref Google Scholar
[51]

D.D. Rees, R.M. Palmer, and S. Moncada, Role of endothelium-derived nitric oxide in the regulation of blood pressure. Proc Natl Acad Sci U.S. A., 1989, 86: 3375-3378.
Crossref Google Scholar
[52]

G. Rodriguez-Gattorno, D. Diaz, L. Rendon, et al., Metallic nanoparticles from spontaneous reduction of silver(Ⅰ) in DMSO. Interaction between nitric oxide and silver nanoparticles. J Phys Chem B, 2002, 106: 2482-2487.
Crossref Google Scholar
[53]

S. Francis, S. Joseph, P.K. Ebey et al., Microwave assisted green synthesis of silver nanoparticles using leaf extract of elephantopus scaber and its environmental and biological applications. Artificial Cells, Nanomedicine, and Biotechnology, 2017: 795-804.
Crossref Google Scholar
[54]

A.K. Gade, P. Bonde, A.P. Ingle, et al., Exploitation of Aspergillus niger for synthesis of silver nanoparticles. J Biobased Mater Bioenergy, 2008, 2(3): 243-247.
Crossref Google Scholar
[55]

J.R. Morones, J.L. Elechiguerra, A. Camacho, et al., The bactericidal effect of silver nanoparticles. Nanotechnology, 2005, 16: 2346-2353.
Crossref Google Scholar
[56]

S. Mukherjee, D. Chowdhury, R. Kotcherlakota, et al., Potential theranostics application of bio-synthesized silver nanoparticles (4-in-1 system). Theranostics, 2014, 4: 316-335.
Crossref Google Scholar
[57]

R. Vivek, R. Thangam, K. Muthuchelian, et al., Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochem, 2012, 47: 2405-2410.
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 10 Issue 3,
September 2018
Pages 279-294
DOI: 10.5101/nbe.v10i3.p279-294
Cite this article:
Venkata SK, Gaddam SA, Kotakadi VS, et al. Multifunctional Silver Nanoparticles by Fruit Extract of Terminalia belarica and their Therapeutic Applications: A 3-in-1 System. Nano Biomedicine and Engineering, 2018, 10(3): 279-294. https://doi.org/10.5101/nbe.v10i3.p279-294

820

Views

38

Downloads

12

Crossref

17

Scopus

Altmetrics
Received: 28 April 2018
Accepted: 03 August 2018
Published: 11 September 2018
© Sucharitha Kumaram Venkata, Susmila Aparna Gaddam, Venkata Subbaiah Kotakadi, and Divi Venkata Ramana Sai Gopal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Return