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Research Article | Open Access

Hepatitis C Virus Core Protein Enhances Natural Killer Cell Depletion of Plasmacytoid Dendritic Cells

Janelle Guy1( ), Emmanuella Sobo2, Lechi Nwanegwo2, Kadir Aslan1( )
Department of Civil Engineering, Morgan State University, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA
Department of Biology, Morgan State University, USA
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Abstract

Investigation of the bi-directional relationship between natural killer (NK) cells and plasmacytoid dendritic cells (pDCs) is critical in understanding antiviral immunity. In the present study, we determined whether the tumor necrosis factor apoptosis-inducing ligand (TRAIL) pathway was responsible for increased apoptosis of pDCs in hepatitis C virus (HCV) infection. We stimulated peripheral blood mononuclear cells (PBMCs) with recombinant HCV core protein within 12 hours to measure the relative expression of tumor necrosis factor apoptosis-inducing receptor 1 (TRAIL-R1) and TRAIL-R2 in pDCs using flow cytometry and image cytometry. We also measured the relative expression of TRAIL in NK cells after stimulation with recombinant HCV core protein using flow cytometry and image cytometry. Using flow cytometry, our results show that within 12 hours of stimulation, HCV core protein increases TRAIL-R1 on pDCs by 0.01%, CD56 expression by 0.66%, and TRAIL expression by 0.66%, in NK cells as compared to unstimulated PBMCs. ELISA and fluorescence spectroscopy results showed that HCV core protein decreases Bcl-2 expression in PBMCs and in pDCs by 36% and 3%, respectively. Our results suggest that HCV core protein increases NK cell deletion of pDCs, independent of the Bcl-2 pathway, contributing to HCV viral escape from immune responses, which may result in chronic HCV infection.

Keywords

HepatitisApoptosisInnate immunityViral pathogenesisHost response

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Nano Biomedicine and Engineering
Volume 11 Issue 1,
March 2019
Pages 44-57
DOI: 10.5101/nbe.v11i1.p44-57
Cite this article:
Guy J, Sobo E, Nwanegwo L, et al. Hepatitis C Virus Core Protein Enhances Natural Killer Cell Depletion of Plasmacytoid Dendritic Cells. Nano Biomedicine and Engineering, 2019, 11(1): 44-57. https://doi.org/10.5101/nbe.v11i1.p44-57

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Received: 01 June 2018
Accepted: 05 February 2019
Published: 01 March 2019
© Janelle Guy, Emmanuella Sobo, Lechi Nwanegwo, and Kadir Aslan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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