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Research Article | Open Access

Rapid Sensing of Biological and Environmental Analytes Using Microwave-Accelerated Bioassays and a MATLAB Application

Enock Bonyi1, Edward Constance1, Zeenat Kukoyi1, Sanjeeda Jafar2, Kadir Aslan1( )
Department of Civil Engineering, Morgan State University, 1700 East Cold Spring Lane, Baltimore, Maryland 21251, USA
Department of Biology, Morgan State University, 1700 East Cold Spring Lane, Baltimore, Maryland 21251, USA
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Abstract

We report a method for rapid detection and analysis of biological and environmental analytes by microwave-accelerated bioassays (MABs) and a novel MATLAB-based image processing of colorimetric signals. In this regard, colorimetric bioassays for histidine-rich protein 2 (HRP-2) and microcystin-leucine arginine (MC-LR) toxin were carried out using MABs and without microwave heating (i.e, gold standard bioassays). Our MATLAB-based detection method is based on the direct correlation of color intensity of a solution calculated from images captured with a smartphone with the concentration of the biomolecule of interest using a MATLAB code developed in-house. We demonstrated that our MATLAB-based detection method can yield bioassay sensitivity comparable to the colorimetric gold standard tool, i.e., UV-Visible spectroscopy. In addition, colorimetric bioassay time for the HRP-2 assay (used in malaria diagnosis) and colorimetric MC-LR bioassay (used in MC-LR toxin diagnosis) was reduced from up to 2 hours at room temperature without microwave heating to 15 minutes using the MABs technique.

Keywords

AnalyteColorimetric bioassaysMicrowave-accelerated bioassaysMicrowave heatingMATLAB

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References

[1]

R. Pickup, Development of molecular methods for the detection of specific bacteria in the environment. Microbiology, 1991, 137(5): 1009-1019.
Crossref Google Scholar
[2]
R.A. Hites, Gas chromatography mass spectrometry. Handbook of instrumental techniques for Analytical Chemistry. Prentice Hall PTR, 1997: 609-626.
[3]

A. El-Aneed, A. Cohen, and J. Banoub, Mass spectrometry, review of the basics: electrospray, MALDI, and commonly used mass analyzers. Applied Spectroscopy Reviews, 2009, 44(3): 210-230.
Crossref Google Scholar
[4]

A. Cavazzini, L. Pasti, A. Massi, et al., Recent applications in chiral high performance liquid chromatography: a review. Analytica Chimica Acta, 2011, 706(2): 205-222.
Crossref Google Scholar
[5]

J. Walker, A.J. Fox, V. Edwards-Jones, et al., Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: Media effects and inter-laboratory reproducibility. Journal of Microbiological Methods, 2002, 48(2-3): 117-126.
Crossref Google Scholar
[6]

B. Abel, T.C. Clement, and K. Aslan, Enhancement of enzymatic colorimetric response by silver island films on high throughput screening microplates. Journal of Immunological Methods, 2014, 411: 43-49.
Crossref Google Scholar
[7]

K. Aslan, P. Holley, and C.D. Geddes, Microwave-accelerated metal-enhanced fluorescence (MAMEF) with silver colloids in 96-well plates: Application to ultra fast and sensitive immunoassays, high throughput screening and drug discovery. Journal of Immunological Methods, 2006. 312(1-2): 137-147.
Crossref Google Scholar
[8]

A.B. Herzog, S.D. McLennan, A.K. Pandey, et al., Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health. Applied and Environmental Microbiology, 2009. 75(19): 6331-6339.
Crossref Google Scholar
[9]

K. Aslan, C.D. Geddes, Microwave-accelerated metal-enhanced fluorescence: Platform technology for ultrafast and ultrabright assays. Analytical Chemistry, 2005, 77(24): 8057-8067.
Crossref Google Scholar
[10]

K. Aslan, Y. Zhang, S. Hibbs, et al., Microwave-accelerated metal-enhanced fluorescence: application to detection of genomic and exosporium anthrax DNA in < 30 seconds. Analyst, 2007, 132(11): 1130-1138.
Crossref Google Scholar
[11]
K. Aslan, C.D. Geddes, Microwave-accelerated metal-enhanced fluorescence. Metal-enhanced Fluorescence, Wiley, 2010: 161-181.
Crossref
[12]

E. Bonyi, Z. Kukoyi, O. Daodu, et al., Metal oxide surfaces for enhanced colorimetric response in bioassays. Colloids and Surfaces B: Biointerfaces, 2017, 154: 331-340.
Crossref Google Scholar
[13]

M. Mohammed, K. Aslan, Design and proof-of-concept use of a circular PMMA platform with 16-well sample capacity for microwave-accelerated bioassays. Nano Biomedicine and Engineering, 2013, 5(1): 10.
Crossref Google Scholar
[14]

M.T. Makler, C.J. Palmer, and A.L. Ager, A review of practical techniques for the diagnosis of malaria. Annals of Tropical Medicine and Parasitology, 1998. 92(4): 419-434.
Crossref Google Scholar
[15]

M. Mohammed, M.F. Syed, and K. Aslan, Microwave-accelerated bioassay technique for rapid and quantitative detection of biological and environmental samples. Biosensors and Bioelectronics, 2016, 75: 420-426.
Crossref Google Scholar
[16]

M. Mohammed, K. Aslan, Rapid and sensitive detection of p53 based on DNA-protein binding interactions using silver nanoparticle films and microwave heating. Nano Biomedicine and Engineering, 2014, 6(3): 76
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 11 Issue 2,
June 2019
Pages 111-123
DOI: 10.5101/nbe.v11i2.p111-123
Cite this article:
Bonyi E, Constance E, Kukoyi Z, et al. Rapid Sensing of Biological and Environmental Analytes Using Microwave-Accelerated Bioassays and a MATLAB Application. Nano Biomedicine and Engineering, 2019, 11(2): 111-123. https://doi.org/10.5101/nbe.v11i2.p111-123

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Received: 20 September 2018
Accepted: 13 February 2019
Published: 15 April 2019
© Enock Bonyi, Edward Constance, Zeenat Kukoyi, Sanjeeda Jafar, and Kadir Aslan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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