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Research Article | Open Access

The Comparison between the Osteogenic Differentiation Potential of Clay-Polyacrylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells

Fatemeh Sadat Tabatabaei Mirakabad1, Simzar Hosseinzadeh2, Hojjat Allah Abbaszadeh3, Maryam Sadat Khoramgah1, Hossein Ghanbarian1, Javad Ranjbari1,†( ), Bahram Kazemi1,†( )
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
Department of tissue engineering and regenerative medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

† These authors contributed equally

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Abstract

Nowadays, bone repair by means of stem cells potential is considered as a new approach in regenerative medicine. Adipose-derived mesenchymal stem cells (AD-MSCs) have been investigated as a plentiful cell source with the ability of osteogenic differentiation which can play an important role in bone tissue engineering applications. Discovering proper elements in combination of scaffolds structure to stimulate osteogenesis in adipose-derived stem cells is one of the major concerns in this issue. Porous polymeric scaffolds such as polyacrylonitrile (PAN) and susceptible nanoparticles have attracted a lot of attention recently due to biodegradability and differentiation potential respectively. In the present study, clay-PAN nanocomposite (CPN) and graphene-PAN scaffold have been electrospuned separately and evaluated from the point of the osteogenic potential in AD-MSCs. The objective of this study was to determine the effect of clay and graphene nanoparticles with PAN nanofibers on the fate of viability and osteogenesis of AD-MSCs. First, isolated mesenchymal cells were characterized by flow cytometry. After cell culture on the surface of scaffolds MTT assay, scanning electron microscope (SEM) and DAPI staining were done. The scaffolds were characterized and osteogenic differentiation potential of AD-MSCs has been investigated. The results have indicated that alkaline phosphatase (ALP) activity, calcium content and collagen expression of cells which cultured on clay-PAN nanofibers were higher than cells which cultured on graphene-PAN scaffold. Taken together, these results suggest that porous nanofiber clay-PAN scaffold can enhance the osteogenic differentiation of AD-MSCs, and can be used as a new biodegradable scaffold for bone tissue engineering applications.

Keywords

ClayGraphenePolyacrylonitrileOsteogenic differentiationMesenchymal stem cell

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Nano Biomedicine and Engineering
Volume 11 Issue 3,
September 2019
Pages 238-253
DOI: 10.5101/nbe.v11i3.p238-253
Cite this article:
Mirakabad FST, Hosseinzadeh S, Abbaszadeh HA, et al. The Comparison between the Osteogenic Differentiation Potential of Clay-Polyacrylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells. Nano Biomedicine and Engineering, 2019, 11(3): 238-253. https://doi.org/10.5101/nbe.v11i3.p238-253

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Received: 22 May 2019
Accepted: 16 August 2019
Published: 16 August 2019
© Fatemeh Sadat Tabatabaei Mirakabad, Simzar Hosseinzadeh, Hojjat Allah Abbaszadeh, Maryam Sadat Khoramgah, Hossein Ghanbarian, Javad Ranjbari, and Bahram Kazemi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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