Home Nano Biomedicine and Engineering Article
PDF (3.4 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript
Research Article | Open Access

Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans

Shaimaa Obaid Hasson1(), Mohammed Jabber Al-Awady2, Mohanad Jawad Kadhim2, Hayder Shkhair Al-Janabi2
Department of Microbiology, College of Veterinary, Al-Qasim Green University, Babylon, Iraq
Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq
Show Author Information

Abstract

Silver nanoparticles have been considered a powerful antimicrobial agents recently especially after increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens required to find a novel path to eradicate that challenge. The present study aims to evaluate the antibacterial activity of biosynthesized silver nanoparticles (AgNPs) using a cell-free extract of Enterobacter cloacae and chemo synthesis by sodium borohydride (NaBH4) on biofilm-forming Pseudomonas oryzihabitans. Antimicrobial effect of silver nanoparticles in both types and in combination with imipenem were evaluated by agar well diffusion method. The results revealed a good response to inhibit biofilm-forming Pseudomonas oryzihabitans growth by silver nanoparticles antibacterial activity in both types (biological and chemical) and in combination with imipenem; the antimicrobial effect was increased and enhanced. In the present study, it was found that the biological and chemical silver nanoparticles were considered a novel and decisive solution against biofilm and multi- drug resistance bacteria with a preference of biological silver nanoparticles.

Keywords

Biological silver NPsChemical silver NPsBiofilmPseudomonas oryzihabitansImipenem

References

[1]

M. Singh, S. Prasad, and S Gambhir, Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Journal of Nanomaterials and Biostructures, 2008, 3(3): 115-122.

Google Scholar
[2]

M. Radzig, V.A. Nadtochenko, O.A. Koksharova, et al., Antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces B: Biointerfaces, 2013, 102: 300-306.

Crossref Google Scholar
[3]

X. Li, H. Chen, Z.S. Chen, et al., Biosynthesis of nanoparticles by microorganisms and their applications. Journal of Nanomaterials, 2011.

Crossref Google Scholar
[4]

R.E. Burrell, A scientific perspective on the use of topical silver preparations. Ostomy Wound Management, 2003, 49(5; SUPP): 19-24.

Google Scholar
[5]

R. Singh, U.U. Shedbalkar, S.A. Wadhwani, et al., Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Applied microbiology and Biotechnology, 2015, 99(11): 4579-4593.

Crossref Google Scholar
[6]

V.K. Sharma, R.A. Yngard, and Y. Lin, Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science, 2009, 145(1-2): 83-96.

Crossref Google Scholar
[7]
J. Sass, Nanotechnology's invisible threat: Small science, big consequences. Proceeding of the 2007 Natural Resources Defense Council. 2007.
[8]

M. Prabakaran, K.V. Nithya, P. Gajendiran, et al., Green synthesis of piperine/triton X-100/silver nanoconjugates: antimicrobial activity and cytotoxicity. Nano Biomedicine and Engineering, 2018, 10(2): 141-148.

Crossref Google Scholar
[9]

S.O. Hasson, M.J. Al-Awady, A.H. Al-Hamadani, et al., Boosting antimicrobial activity of imipenem in combination with silver nanoparticles towards S. fonticola and Pantoea sp. Nano Biomed. Eng., 2019, 11(2): 200-214.

Crossref Google Scholar
[10]

V. Kostenko, J.T. Lyczak, K. Martinuzzi, et al., Impact of silver-containing wound dressings on bacterial biofilm viability and susceptibility to antibiotics during prolonged treatment. Antimicrobial Agents and Chemotherapy, 2010, 54(12): 5120-5131.

Crossref Google Scholar
[11]
M.A. Theivasanthi, Anti-bacterial studies of silver nanoparticles. arXiv. org, 2011: arXiv: 1101.0348 [physics. gen-ph].
[12]

M.J. Al-Awady, A.A.J.N.B.E. Balakit, Investigation of anti-MRSA and anticancer activity of eco-friendly synthesized silver nanoparticles from palm dates extract. Nano Biomed. Eng. , 2019, 10(2): 157-169.

Crossref Google Scholar
[13]

A.R. Shahverdi, S.S. Minaeian, H.R. Jamalifar, et al., Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Process Biochemistry, 2007, 42(5): 919-923.

Crossref Google Scholar
[14]

M. Husseiny, M.A.B. El-Aziz, and M.A.Y. Mahmoud, Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2007, 67(3-4): 1003-1006.

Crossref Google Scholar
[15]

N.S. Shaligram, M.B. Bule, R. Singhal, et al., Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain. Process Biochemistry, 2009, 44(8): 939-943.

Crossref Google Scholar
[16]

M. Fu, Q. Li, D. Sun, et al., Rapid preparation process of silver nanoparticles by bioreduction and their characterizations. Chinese Journal of Chemical Engineering, 2006, 14(1): 114-117.

Crossref Google Scholar
[17]

T. Ogi, Room-temperature synthesis of gold nanoparticles and nanoplates using Shewanella algae cell extract. Journal of Nanoparticle Research, 2010, 12(7): 2531-2539.

Crossref Google Scholar
[18]

Z. Sadowski, Synthesis of silver nanoparticles using microorganisms. Materials Science-Poland, 2008, 26(2): 419-424.

Google Scholar
[19]

K.N. Thakkar, S.S. Mhatre, and R.Y. Parikh, Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 2010, 6(2): 257-262.

Crossref Google Scholar
[20]
M. Rai, N. Duran, Metal nanoparticles in microbiology. Springer Science & Business Media, 2011.
Crossref
[21]

G. Sharma, A.S. Kumar, S. Naushad, et al., Novel development of nanoparticles to bimetallic nanoparticles and their composites: A review. Journal of King Saud University-Science, 2017.

Google Scholar
[22]

S. Neethirajan, M.A. Clond, and A. Vogt, Medical biofilms - nanotechnology approaches. Journal of Biomedical Nanotechnology, 2014, 10(10): 2806-2827.

Crossref Google Scholar
[23]

M. Rai, A. Yadav, and A. Gade, Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 2009, 27(1): 76-83.

Crossref Google Scholar
[24]

H.H. Lara, E.N. Garza-Treviño, L. Ixtepan-Turrent, et al., Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. Journal of Nanobiotechnology, 2011, 9(1): 30.

Crossref Google Scholar
[25]

S. Tang, J. Zheng, Antibacterial activity of silver nanoparticles: Structural effects. Advanced Healthcare Materials, 2018: 1701503.

Crossref Google Scholar
[26]

S.O. Hasson, Phenotypic and genotypic detection of biofilm formation Pseudomonas oryzihabitance and susceptibility to antibiotics. Nano Biomed. Eng. , 2019, 11(1): 11-17.

Crossref Google Scholar
[27]

I.H. Al-Azawi, A.H. Al-Hamadani, and S.O. Hasson, Association between biofilm formation and susceptibility to antibiotics in Staphylococcus lentus isolated from urinary catheterized patients. Nano Biomed. Eng. , 2018, 10(2): 97-103.

Crossref Google Scholar
[28]

S.O. Hasson, A.H. Al-Hamadani, and I.H. Al-Azawi, Occurrence of biofilm formation in Serratia fonticola and Pantoea sp. isolates among urinary catheterized patients. Nano Biomed. Eng. , 2018, 10(3): 295-304.

Crossref Google Scholar
[29]
CLSI, Performance standards for antimicrobial susceptibility testing. M100. Clinical and Laboratory Standards Institute, Wayne, PA., 2017.
[30]

E.Z. Gomaa, Antimicrobial, antioxidant and antitumor activities of silver nanoparticles synthesized by Allium cepa extract: A green approach. Journal of Genetic Engineering and Biotechnology, 2017, 15(1): 49-57.

Crossref Google Scholar
[31]
P. Verma, Methods for determining bactericidal activity and antimicrobial interactions: synergy testing, time-kill curves, and population analysis. CRC Press, New York, USA, 2007: 275-290.
Crossref
[32]

F.P. Mehr, M. Khanjani, and P. Vatani, Synthesis of nano-Ag particles using sodium borohydride. Oriental Journal of Chemistry, 2015, 31(3): 1831-1833.

Crossref Google Scholar
[33]

K. Kalimuthu, Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloids and Surfaces B: Biointerfaces, 2008, 65(1): 150-153.

Crossref Google Scholar
[34]

N. Durán, P.D. Marcato, O.L. Alves, et al., Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. Journal of Nanobiotechnology, 2005, 3(1): 8.

Crossref Google Scholar
[35]

C. Wang, Green synthesis of silver nanoparticles by Bacillus methylotrophicus, and their antimicrobial activity. Artificial Cells, Nanomedicine, and Biotechnology, 2016, 44(4): 1127-1132.

Google Scholar
[36]

B. Kumar, K. Smita, L. Cumbal, et al., Fabrication of silver nanoplates using Nephelium lappaceum (Rambutan) peel: a sustainable approach. Journal of Molecular Liquids, 2015, 211: 476-480.

Crossref Google Scholar
[37]

S. Gurunathan, J.W. Han, D.-N. Kwon, et al., Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria. Nanoscale Research Letters, 2014, 9(1): 373.

Crossref Google Scholar
[38]

R. Ghotaslou, Z. Bahari, The in vitro effects of silver nanoparticles on bacterial biofilms. The Journal of Microbiology, Biotechnology and Food Sciences, 2017, 6(4): 1077.

Crossref Google Scholar
[39]

G. Martinez-Castanon, N. Nino-Martinez, F. Martinez-Gutierrez, et al., Synthesis and antibacterial activity of silver nanoparticles with different sizes. Journal of Nanoparticle Research, 2008, 10(8): 1343-1348.

Crossref Google Scholar
[40]

M. Ferrari, Nanogeometry: Beyond drug delivery. Nature Nanotechnology, 2008, 3(3): 131.

Crossref Google Scholar
[41]

W. Jiang, B.Y.S. Kim, R.J.T. Chan, et al., Nanoparticle-mediated cellular response is size-dependent. Nature Nanotechnology, 2008, 3(3): 145.

Crossref Google Scholar
[42]

I. Sondi, B. Salopek-Sondi, Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science, 2004, 275(1): 177-182.

Crossref Google Scholar
[43]

N. Beyth, Y.D. Houri-Haddad, A. Khan, et al., Alternative antimicrobial approach: Nano-antimicrobial materials. Evidence-Based Complementary and Alternative Medicine, 2015.

Crossref Google Scholar
[44]

C. Buzea, I.I. Pacheco, and K. Robbie, Nanomaterials and nanoparticles: sources and toxicity. Biointerphases, 2007, 2(4): MR17-MR71.

Crossref Google Scholar
[45]

C.-N. Lok, C.-M. Ho, R. Chen, et al., Proteomic analysis of the mode of antibacterial action of silver nanoparticles. Journal of Proteome Research, 2006, 5(4): 916-924.

Crossref Google Scholar
[46]

S. Shrivastava, T.R. Bera, A. Singh, et al., Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology, 2007, 18(22): 225103.

Crossref Google Scholar
[47]

Y.-G. Yuan, Q.-L. Peng, and S. Gurunathan, Effects of silver nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa from mastitis-infected goats: An alternative approach for antimicrobial therapy. International Journal of Molecular Sciences, 2017, 18(3): 569.

Crossref Google Scholar
[48]

J. Jiang, G. Oberdörster, and P. Biswas, Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. Journal of Nanoparticle Research, 2009, 11(1): 77-89.

Crossref Google Scholar
[49]

B. Le Ouay, F. Stellacci, Antibacterial activity of silver nanoparticles: A surface science insight. Nano Today, 2015, 10(3): 339-354.

Crossref Google Scholar
[50]

S. Gurunathan, Rapid biological synthesis of silver nanoparticles and their enhanced antibacterial effects against Escherichia fergusonii and Streptococcus mutans. Arabian Journal of Chemistry, 2014.

Google Scholar
Nano Biomedicine and Engineering
Volume 11 Issue 3,
September 2019
Pages 297-305
DOI: 10.5101/nbe.v11i3.p297-305
Cite this article:
Hasson SO, Al-Awady MJ, Kadhim MJ, et al. Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans. Nano Biomedicine and Engineering, 2019, 11(3): 297-305. https://doi.org/10.5101/nbe.v11i3.p297-305
Metrics & Citations  
Article History
Copyright
Rights and Permissions
Return