Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming <i>Pseudomonas oryzihabitans</i>

Shaimaa Obaid Hasson; Mohammed Jabber Al-Awady; Mohanad Jawad Kadhim; Hayder Shkhair Al-Janabi

doi:10.5101/nbe.v11i3.p297-305

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Research Article | Open Access

Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans

Shaimaa Obaid Hasson^¹(), Mohammed Jabber Al-Awady^², Mohanad Jawad Kadhim^², Hayder Shkhair Al-Janabi^²

Department of Microbiology, College of Veterinary, Al-Qasim Green University, Babylon, Iraq

Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq

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Abstract

Silver nanoparticles have been considered a powerful antimicrobial agents recently especially after increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens required to find a novel path to eradicate that challenge. The present study aims to evaluate the antibacterial activity of biosynthesized silver nanoparticles (AgNPs) using a cell-free extract of Enterobacter cloacae and chemo synthesis by sodium borohydride (NaBH₄) on biofilm-forming Pseudomonas oryzihabitans. Antimicrobial effect of silver nanoparticles in both types and in combination with imipenem were evaluated by agar well diffusion method. The results revealed a good response to inhibit biofilm-forming Pseudomonas oryzihabitans growth by silver nanoparticles antibacterial activity in both types (biological and chemical) and in combination with imipenem; the antimicrobial effect was increased and enhanced. In the present study, it was found that the biological and chemical silver nanoparticles were considered a novel and decisive solution against biofilm and multi- drug resistance bacteria with a preference of biological silver nanoparticles.

Keywords

Biological silver NPs Chemical silver NPs Biofilm Pseudomonas oryzihabitansImipenem

References

[1]

M. Singh, S. Prasad, and S Gambhir, Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Journal of Nanomaterials and Biostructures, 2008, 3(3): 115-122.

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[2]

M. Radzig, V.A. Nadtochenko, O.A. Koksharova, et al., Antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces B: Biointerfaces, 2013, 102: 300-306.

AgNPs con. (μg/mL)	Zone of inhibition (mean ± SE)	p-value
170	21.0000 ± 1.73205	0.03
150	16.0000 ± 0.91287
130	15.2500 ± 0.94648
110	14.7500 ± 0.75000
90	13.0000 ± 1.35401

AgNPs type	Zone of inhibition (Mean ± SE)	p-value
Biological	16.000 ± 0.777	0.420
Chemical	15.000 ± 0.865	0.420

AgNPs/imipenem con. (μg/mL)	Zone of inhibition (mean ± SE)	p-value
170/8	24.0000 ± 0.00000	< 0.01
150/4	21.0000 ± 0.00000
130/2	16.3333 ± 0.33333
110/1	17.6667 ± 0.88192
90/0.5	13.6667 ± 0.33333
70/0.25	13.3333 ± 0.33333
50/0.125	13.3333 ± 0.33333

AgNPs/imipenem conc. (μg/mL)	Zone of inhibition (mean ± SE)	p-value
85/8	26.6667 ± 0.333	< 0.01
65/4	25.3333 ± 0.333
45/2	23.3333 ± 0.333
25/1	19.6667 ± 0.333
5/0.5	13.6667 ± 0.333

Con.	P. oryzihabitans 1	P. oryzihabitans 2	P. oryzihabitans 3
170/8	0	9	9
150/4	50	40	16
130/2	14.2	21.4	6.6
110/1	35.7	28.5	14.2
90/0.5	18	16.6	16.6

Con.	P. oryzihabitans 1	P. oryzihabitans 2	P. oryzihabitans 3
85/8	92.8	80	71.4
65/4	100	85.7	78.5
45/2	71.4	76.6	76.9
25/1	53.8	53.8	46.1
5/0.5	8.3	16.6	16.6