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Research Article | Open Access

Effect of Antimonial Therapy on Levels of (TNF-α and IL-1β) Cytokines in Cutaneous Leishmaniasis Patients in Iraq

Hasan Raheem Khudhur1( ), Ahmed Abbas Hasan2, Rawaa Adday Ali3
Ministry of Education, Iraq
College of Health and Medical Techniques, Kufa, Al-Furat Al-Awsat Technical University, 31003 Al-Kufa, Iraq
AL-Qasim Green University, College of Veterinary Medicine, Microbiology Department, Iraq
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Abstract

This study aimed to study the effective antimonial therapy on circulating levels proinflammatory cytokines, and their effect on susceptibility to cutaneous leishmaniasis (CL) infection in the Iraqi population. Fifty CssL patients were treated with pentavalent antimonial salts (pentostam) for 7 weeks. Leishmania species were identified by Nested-Polymerase chain reaction method, and in all the cases the strains corresponded to Leishmania major. Circulating plasma levels of the proinflammatory cytokines interleukin-1 and tumor necrosis factor-alpha were determined for CL patients and healthy subjects before and during 7 weeks after the treatment were started. Concentrations were detected by enzyme-linked immunosorbent assay technique using a quantitative sandwich enzyme immunoassay technique. Proinflammatory cytokines significantly increased after 7 days postinfection compared to levels in the pretreatment patients. It was clearly recorded in the present study that the level of interleukin-1 and tumor necrosis factor-alpha in the serum of CL patients was responsively increasing with the antimonial therapy dose.

Keywords

Cutaneous leishmaniasisAntimonial therapyTNF-α and IL-1βIraq

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Nano Biomedicine and Engineering
Volume 11 Issue 4,
December 2019
Pages 340-346
DOI: 10.5101/nbe.v11i4.p340-346
Cite this article:
Khudhur HR, Hasan AA, Ali RA. Effect of Antimonial Therapy on Levels of (TNF-α and IL-1β) Cytokines in Cutaneous Leishmaniasis Patients in Iraq. Nano Biomedicine and Engineering, 2019, 11(4): 340-346. https://doi.org/10.5101/nbe.v11i4.p340-346

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Received: 25 July 2019
Accepted: 11 November 2019
Published: 11 November 2019
© Hasan Raheem Khudhur, Ahmed Abbas Hasan, and Rawaa Adday Ali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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