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Research Article | Open Access

Preparation and Characterization of Methotrexate Loaded Polymeric Nanoparticles by Nanoprecipitation Technique

Ayesha Siddiqua Gazi, Abbaraju Krishnasailaja( )
RBVRR Women's College of Pharmacy, Osmania University, Barkatpura, Hyderabad, India
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Abstract

The aim of this study was to develop and characterize methotrexate-loaded polymeric nanoparticles by nanoprecipitation technique. Eudragit? S 100 and ethyl cellulose were employed to develop methotrexate-loaded nanoparticles by nanoprecipitation technique. Six different formulations (f1, f2, f3, f4, f5 and f6) were prepared with each polymer by varying the drug to polymer ratios (1:1, 1:1.5, 1:2, 1:3, 1.5:1 and 2:1). Dimethyl sulfoxide (DMSO) was used as a solvent and Tween? 20 as a surfactant. Among the six formulations of polymeric nanoparticles prepared by nanoprecipitation technique, F4 formulation was considered as a best formulation with each polymer. Based on comparison results of mean particle diameter, zeta potential, drug content and entrapment efficiency, Eudragit? S 100 was considered to be the most suitable polymer for preparation of methotrexate-loaded nanoparticles by nanoprecipitation technique. Based upon the evaluation studies, the best formulation was characterized for scanning electron microscopy (SEM), particle size, zeta potential and anti-cancer activity in MCF-7 cell line by MTT assay.

Keywords

Polymeric nanoparticlesMethotrexateNanoprecipitation technique

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Nano Biomedicine and Engineering
Volume 11 Issue 4,
December 2019
Pages 351-360
DOI: 10.5101/nbe.v11i4.p351-360
Cite this article:
Gazi AS, Krishnasailaja A. Preparation and Characterization of Methotrexate Loaded Polymeric Nanoparticles by Nanoprecipitation Technique. Nano Biomedicine and Engineering, 2019, 11(4): 351-360. https://doi.org/10.5101/nbe.v11i4.p351-360

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Received: 08 September 2019
Accepted: 18 November 2019
Published: 18 November 2019
© Ayesha Siddiqua Gazi, Abbaraju Krishnasailaja.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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